#!/usr/bin/env perl # Copyright (C) 2007-2009 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Read BLAST records in tabular format from one or more files. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Maasha::Biopieces; use Maasha::Filesys; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $file, $record, $line, @fields, $data_in, $num ); $options = Maasha::Biopieces::parse_options( [ { long => 'data_in', short => 'i', type => 'files!', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'num', short => 'n', type => 'uint', mandatory => 'no', default => undef, allowed => undef, disallowed => '0' }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { Maasha::Biopieces::put_record( $record, $out ); } if ( $options->{ 'data_in' } ) { $num = 1; $data_in = Maasha::Filesys::files_read_open( $options->{ 'data_in' } ); while ( $line = <$data_in> ) { chomp $line; next if $line =~ /^#/; @fields = split /\t/, $line; $record->{ "REC_TYPE" } = "BLAST"; $record->{ "Q_ID" } = $fields[ 0 ]; $record->{ "S_ID" } = $fields[ 1 ]; $record->{ "IDENT" } = $fields[ 2 ]; $record->{ "ALIGN_LEN" } = $fields[ 3 ]; $record->{ "MISMATCHES" } = $fields[ 4 ]; $record->{ "GAPS" } = $fields[ 5 ]; $record->{ "Q_BEG" } = $fields[ 6 ] - 1; # BLAST is 1-based $record->{ "Q_END" } = $fields[ 7 ] - 1; # BLAST is 1-based $record->{ "S_BEG" } = $fields[ 8 ] - 1; # BLAST is 1-based $record->{ "S_END" } = $fields[ 9 ] - 1; # BLAST is 1-based $record->{ "E_VAL" } = $fields[ 10 ]; $record->{ "BIT_SCORE" } = $fields[ 11 ]; if ( $record->{ "S_BEG" } > $record->{ "S_END" } ) { $record->{ "STRAND" } = '-'; ( $record->{ "S_BEG" }, $record->{ "S_END" } ) = ( $record->{ "S_END" }, $record->{ "S_BEG" } ); } else { $record->{ "STRAND" } = '+'; } Maasha::Biopieces::put_record( $record, $out ); last if $options->{ "num" } and $num == $options->{ "num" }; $num++; } close $data_in; } Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__