#!/usr/bin/env perl # Copyright (C) 2007-2010 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Read 454 entries from fasta and quality file. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Data::Dumper; use Maasha::Biopieces; use Maasha::Filesys; use Maasha::Fasta; use Maasha::Fastq; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $record, $data_in, $qual_in, $num, $fasta, $qual, @seqs, @scores, $i ); $options = Maasha::Biopieces::parse_options( [ { long => 'data_in', short => 'i', type => 'file!', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'qual_in', short => 'q', type => 'file!', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'num', short => 'n', type => 'uint', mandatory => 'no', default => undef, allowed => undef, disallowed => '0' }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { Maasha::Biopieces::put_record( $record, $out ); } if ( $options->{ 'data_in' } ) { $data_in = Maasha::Filesys::file_read_open( $options->{ 'data_in' } ); $qual_in = Maasha::Filesys::file_read_open( $options->{ 'qual_in' } ); $num = 1; while ( $fasta = Maasha::Fasta::get_entry( $data_in ) ) { $qual = get_qual( $qual_in ); check_names( $fasta, $qual ); check_lengths( $fasta, $qual ); $record = { SEQ_NAME => $fasta->[ 0 ], SEQ => $fasta->[ 1 ], SEQ_LEN => length $fasta->[ 1 ], SCORES => $qual->[ 1 ], }; Maasha::Biopieces::put_record( $record, $out ); last if $options->{ "num" } and $num == $options->{ "num" }; $num++; } close $data_in; close $qual_in; } Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< sub get_qual { # Martin A. Hansen, May 2010. # Get the next 454 quality entry from an open file. my ( $fh, # file handle ) = @_; # Returns list. my ( $block, $name, $qual, $scores, $entry ); local $/ = "\n>"; while ( $block = <$fh> ) { chomp $block; last if $block !~ /^\s+$/; } return if not defined $block; $block =~ /^>?(.+)\n/m; $name = $1; $qual = $'; local $/ = "\n"; chomp $qual; $name =~ tr/\r//d; $qual =~ tr/ \n\r/;;;/; $qual =~ s/;;/;/g; $scores = Maasha::Fastq::dec_str2solexa_str( $qual ); $entry = [ $name, $scores ]; return wantarray ? @{ $entry } : $entry; } sub check_names { # Martin A. Hansen, May 2010. # Check if the names of the fasta and qual entries match # and raise an error if they don't. my ( $fasta, # fasta entry $qual, # qual entry ) = @_; # Returns nothing. my ( $f_name, $q_name ); if ( $fasta->[ 0 ] =~ /^([^ ]+)/ ) { $f_name = $1; } if ( $qual->[ 0 ] =~ /^([^ ]+)/ ) { $q_name = $1; } Maasha::Common::error( qq(names don't match "$fasta->[ 0 ]" ne "$qual->[ 0 ]") ) if $f_name ne $q_name; } sub check_lengths { # Martin A. Hansen, April 2011. # Check if the lengths of the fasta and qual strings are the same # and raise an error if not. my ( $fasta, # fasta entry $qual, # qual entry ) = @_; # Returns nothing. my ( $f_len, $q_len ); $f_len = length $fasta->[ 1 ]; $q_len = length $qual->[ 1 ]; Maasha::Common::error( qq(lengths don't match "$f_len" != "$q_len") ) if $f_len != $q_len; } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__