#!/usr/bin/env perl # Copyright (C) 2007-2009 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Generate a dotplot of matches in the stream. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Maasha::Biopieces; use Maasha::Plot; use Maasha::Filesys; use IPC::Open2; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $default, $terminals, $record, @data, $fh, $result, %data_hash, $tmp_dir ); $default = "plot_matches"; $terminals = "dumb,x11,aqua,post,svg,png"; $options = Maasha::Biopieces::parse_options( [ { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'data_out', short => 'o', type => 'file', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'terminal', short => 't', type => 'string', mandatory => 'no', default => 'dumb', allowed => $terminals, disallowed => undef }, { long => 'direction', short => 'd', type => 'string', mandatory => 'no', default => 'both', allowed => 'both,forward,reverse', disallowed => undef }, { long => 'title', short => 'T', type => 'string', mandatory => 'no', default => $default, allowed => undef, disallowed => undef }, { long => 'xlabel', short => 'X', type => 'string', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'ylabel', short => 'Y', type => 'string', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { if ( defined $record->{ "Q_BEG" } and defined $record->{ "S_BEG" } and $record->{ "Q_END" } and $record->{ "S_END" } ) { push @data, $record; } Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" }; } $options->{ "xlabel" } ||= $data[ 0 ]->{ "Q_ID" }; $options->{ "ylabel" } ||= $data[ 0 ]->{ "S_ID" }; $tmp_dir = Maasha::Biopieces::get_tmpdir(); $result = dotplot_matches( \@data, $options, $tmp_dir ); $fh = Maasha::Biopieces::write_stream( $options->{ "data_out" } ); print $fh "$_\n" foreach @{ $result }; close $fh; Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> SUBROUTINES <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< sub dotplot_matches { # Martin A. Hansen, August 2007. # Generates a dotplot from a list of matches using Gnuplot. my ( $matches, # list of hashrefs. $options, # options hash $tmp_dir, # temporary directory ) = @_; # Returns list. my ( $forward_file, $backward_file, $pid, $fh_forward, $fh_backward, $fh_in, $fh_out, $cmd, $match, $line, @lines, $q_max, $s_max ); $forward_file = "$tmp_dir/match_f.tab"; $backward_file = "$tmp_dir/match_r.tab"; $fh_forward = Maasha::Filesys::file_write_open( $forward_file ); $fh_backward = Maasha::Filesys::file_write_open( $backward_file ); $q_max = 0; $s_max = 0; foreach $match ( @{ $matches } ) { if ( ($match->{ "DIR" } and $match->{ "DIR" } =~ /^f/) or ($match->{ "STRAND" } and $match->{ "STRAND" } eq '+') ) { print $fh_forward join( "\t", $match->{ "Q_BEG" } + 1, $match->{ "S_BEG" } + 1 ), "\n"; print $fh_forward join( "\t", $match->{ "Q_END" } + 1, $match->{ "S_END" } + 1 ), "\n"; print $fh_forward "\n\n"; } else { print $fh_backward join( "\t", $match->{ "Q_BEG" } + 1, $match->{ "S_END" } + 1 ), "\n"; print $fh_backward join( "\t", $match->{ "Q_END" } + 1, $match->{ "S_BEG" } + 1 ), "\n"; print $fh_backward "\n\n"; } $q_max = $match->{ "Q_END" } if $match->{ "Q_END" } > $q_max; $s_max = $match->{ "S_END" } if $match->{ "S_END" } > $s_max; } $q_max++; $s_max++; close $fh_forward; close $fh_backward; $cmd = "gnuplot -persist"; $pid = open2( $fh_out, $fh_in, $cmd ); print $fh_in "set terminal $options->{ 'terminal' }\n"; print $fh_in "set xrange [1:$q_max]\n"; print $fh_in "set yrange [1:$s_max]\n"; print $fh_in "set title \"$options->{ 'title' }\"\n" if $options->{ "title" }; print $fh_in "set xlabel \"$options->{ 'xlabel' }\"\n" if $options->{ "xlabel" }; print $fh_in "set ylabel \"$options->{ 'ylabel' }\"\n" if $options->{ "ylabel" }; print $fh_in "unset key\n"; if ( $options->{ "terminal" } ne "dumb" ) { print $fh_in "set style line 1 linetype 1 linecolor rgb \"green\" linewidth 2 pointtype 6 pointsize default\n"; print $fh_in "set style line 2 linetype 1 linecolor rgb \"red\" linewidth 2 pointtype 6 pointsize default\n"; } print $fh_in "set xtics border out\n"; print $fh_in "set ytics border out\n"; print $fh_in "set grid\n"; if ( $options->{ "direction" } =~ /^b/ ) { print $fh_in qq(plot "$forward_file" with lines ls 1, "$backward_file" with lines ls 2\n); } elsif ( $options->{ "direction" } =~ /^f/ ) { print $fh_in qq(plot "$forward_file" with lines ls 1\n); } elsif ( $options->{ "direction" } =~ /^r/ ) { print $fh_in qq(plot "$backward_file" with lines ls 2\n); } close $fh_in; while ( $line = <$fh_out> ) { chomp $line; push @lines, $line; } close $fh_out; waitpid $pid, 0; unlink $forward_file; unlink $backward_file; return wantarray ? @lines : \@lines; } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__