#!/usr/bin/env ruby # Copyright (C) 2007-2012 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Order records with pair end sequence data. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'pp' options = Biopieces.options_parse(ARGV) hash1 = {} hash2 = {} Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record[:SEQ_NAME] case record[:SEQ_NAME] when /^(.+)\/(\d)$/ # Illumina 1.5 name = $1.to_sym pair = $2.to_i when /^(.+) (\d):/ # Illumina 1.8 name = $1.to_sym pair = $2.to_i else $stderr.puts "WARNING: Unmatched sequence name: #{record[:SEQ_NAME]}" end if pair == 1 if hash2[name] record[:ORDER] = "paired" hash2[name][:ORDER] = "paired" output.puts record output.puts hash2[name] hash2.delete(name) else hash1[name] = record end elsif pair == 2 if hash1[name] hash1[name][:ORDER] = "paired" record[:ORDER] = "paired" output.puts hash1[name] output.puts record hash1.delete(name) else hash2[name] = record end end end end hash1.each_value do |record| record[:ORDER] = "orphan 1" output.puts record end hash2.each_value do |record| record[:ORDER] = "orphan 2" output.puts record end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__