#!/usr/bin/env ruby # Copyright (C) 2007-2012 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Order records with pair end sequence data. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'gdbm' require 'pp' ILLUMINA15 = 15 ILLUMINA18 = 18 options = Biopieces.options_parse(ARGV) tmpdir = Biopieces.mktmpdir #tmpdir = "Sletmig" tmpfile = File.join(tmpdir, "data.gdbm") db = GDBM.new(tmpfile) Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record[:SEQ_NAME] db[record[:SEQ_NAME]] = Marshal.dump(record) else output.puts record end end skip = {} db.keys.each do |seq_name| next if skip[seq_name] case seq_name when /^(.+)\/(\d)$/ # Illumina 1.5 type = ILLUMINA15 name = $1 pair = $2.to_i when /^(.+) (\d):/ # Illumina 1.8 type = ILLUMINA18 name = $1.to_sym pair = $2.to_i else $stderr.puts "WARNING: Unmatched sequence name: #{record[:SEQ_NAME]}" end pair2 = (pair == 1) ? 2 : 1 if type == ILLUMINA15 seq_name2 = "#{name}/#{pair2}" else seq_name2 = seq_name.sub(/^(.+) \d:/, "#{$1} #{pair2}:") end record1 = Marshal.load(db[seq_name]) record2 = db[seq_name2] record2 = Marshal.load(record2) unless record2.nil? if record2.nil? record1[:ORDER] = "orphan#{pair}" output.puts record1 else record1[:ORDER] = "paired" record2[:ORDER] = "paired" output.puts record1 output.puts record2 skip[seq_name2] = true end end end db.close FileUtils.remove_entry_secure(tmpfile, true) # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__