#!/usr/bin/env ruby # Copyright (C) 2007-2013 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Find paired end orphan in records with sequence data. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'pp' options = Biopieces.options_parse(ARGV) class Illumina def self.name_match?(name1, name2) if name1[-2] == '/' name1[0 .. -3 ] == name2[0 .. -3] # Solexa and Illumina <= 1.5 version names else name1.split(" ").first == name2.split(" ").first # Illumina > 1.5 version names end end end records = [] Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record[:SEQ_NAME] records << record if records.size == 2 if Illumina.name_match?(records.first[:SEQ_NAME], records.last[:SEQ_NAME]) records.first[:TYPE] = "paired" records.last[:TYPE] = "paired" output.puts records.first output.puts records.last records = [] else records.first[:TYPE] = "orphan" output.puts records.first records.shift end end end end records.each do |record| record[:TYPE] = "orphan" output.puts record end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__