#!/usr/bin/env ruby # Copyright (C) 2007-2011 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Find genes in prokaryotic genomic sequences in the stream. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/fasta' require 'maasha/prodigal' casts = [] casts << {:long=>'full', :short=>'f', :type=>'flag', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} casts << {:long=>'procedure', :short=>'p', :type=>'string', :mandatory=>true, :default=>'single', :allowed=>'single,meta', :disallowed=>nil} options = Biopieces.options_parse(ARGV, casts) tmpdir = Biopieces.mktmpdir infile = File.join(tmpdir, "in.fna") outfile = File.join(tmpdir, "out.prodigal") Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| Fasta.open(infile, "w") do |fasta_io| input.each_record do |record| output.puts record if record[:SEQ_NAME] and record[:SEQ] seq = Seq.new_bp(record) fasta_io.puts seq.to_fasta end end end prodigal = Prodigal.new(infile, outfile, options) prodigal.run prodigal.each do |record| output.puts record end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__