#!/usr/bin/env ruby # Copyright (C) 2007-2010 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Split sequences in the stream at a given restriction enzyme's cleavage points. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/seq' casts = [] casts << {:long=>'pattern', :short=>'p', :type=>'string', :mandatory=>true, :default=>nil, :allowed=>nil, :disallowed=>nil} casts << {:long=>'cut_pos', :short=>'c', :type=>'int', :mandatory=>true, :default=>nil, :allowed=>nil, :disallowed=>nil} options = Biopieces.options_parse(ARGV, casts) Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record[:SEQ_NAME] and record[:SEQ] seq = Seq.new_bp(record) seq.each_digest(options[:pattern].to_s, options[:cut_pos]) do |digest| new_record = digest.to_bp if new_record[:SEQ_NAME] =~ /\[(\d+)-(\d+)\]$/ s_beg = $1 s_end = $2 end new_record[:S_BEG] = s_beg new_record[:S_END] = s_end new_record[:REC_TYPE] = "DIGEST" output.puts new_record end else output.puts record end end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__