#!/usr/bin/env perl # Copyright (C) 2007-2009 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Create a BLAST database from sequences in stream for use with [blast_seq]. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Maasha::Seq; use Maasha::Fasta; use Maasha::Biopieces; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $record, $tmp_dir, $file_tmp, $fh_tmp, $entry, $seq_type, $arg ); $options = Maasha::Biopieces::parse_options( [ { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'directory', short => 'd', type => 'dir', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'index_name', short => 'i', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); $tmp_dir = Maasha::Biopieces::get_tmpdir(); $file_tmp = "$tmp_dir/$options->{ 'index_name' }"; $fh_tmp = Maasha::Filesys::file_write_open( $file_tmp ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { if ( $entry = Maasha::Fasta::biopiece2fasta( $record ) ) { $seq_type = Maasha::Seq::seq_guess_type( $record->{ 'SEQ' } ) if not $seq_type; Maasha::Fasta::put_entry( $entry, $fh_tmp ); } Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" }; } close $fh_tmp; $arg = "-t $options->{ 'index_name' }"; if ( $seq_type eq "PROTEIN" ) { $arg .= " -p T"; } else { $arg .= " -p F"; } $arg .= " -v 12000"; $arg .= " -i $file_tmp"; if ( $options->{ 'verbose' } ) { print STDERR "Running: formatdb $arg\n"; Maasha::Common::run( "formatdb", "$arg" ); } else { Maasha::Common::run( "formatdb", "$arg -l /dev/null" ); } Maasha::Filesys::dir_create_if_not_exists( $options->{ 'directory' } ); unlink $file_tmp; system( "cp $tmp_dir/* $options->{ 'directory' }" ); Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__