#!/usr/bin/env perl # Copyright (C) 2007-2010 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Calculate fixedstep entries from records in the stream. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Maasha::Biopieces; use Maasha::Filesys; use Maasha::UCSC::BED; use Maasha::UCSC::Wiggle; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $record, $tmp_dir, $bed_file, $fh_in, $fh_out, $file_hash, $chr, $bed_entry, $fixedstep_file, $fixedstep_entry ); $options = Maasha::Biopieces::parse_options( [ { long => 'check', short => 'C', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'log10', short => 'l', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); $tmp_dir = Maasha::Biopieces::get_tmpdir(); $bed_file = "$tmp_dir/calc_fixedstep.bed"; $fh_out = Maasha::Filesys::file_write_open( $bed_file ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { if ( $bed_entry = Maasha::UCSC::BED::biopiece2bed( $record ) ) { Maasha::UCSC::BED::bed_entry_put( $bed_entry, $fh_out, undef, $options->{ 'check' } ); } Maasha::Biopieces::put_record( $record, $out ); } close $fh_out; $file_hash = Maasha::UCSC::BED::bed_file_split_on_chr( $bed_file, $tmp_dir ); unlink $bed_file; foreach $chr ( sort keys %{ $file_hash } ) { $bed_file = $file_hash->{ $chr }; $fixedstep_file = Maasha::UCSC::Wiggle::fixedstep_calc( $bed_file, $chr, $options->{ 'log10' } ); #$fixedstep_file = "$bed_file.fixedstep"; # Maasha::Common::run( "bed2fixedstep", "< $bed_file > $fixedstep_file" ); $fh_in = Maasha::Filesys::file_read_open( $fixedstep_file ); while ( $fixedstep_entry = Maasha::UCSC::Wiggle::fixedstep_entry_get( $fh_in ) ) { if ( $record = Maasha::UCSC::Wiggle::fixedstep2biopiece( $fixedstep_entry ) ) { Maasha::Biopieces::put_record( $record, $out ); } } close $fh_in; unlink $bed_file; unlink $fixedstep_file; } Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__