#!/usr/bin/env ruby # Copyright (C) 2007-2013 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Assemble ordered overlapping pair-end sequences in the stream. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/seq' require 'maasha/seq/assemble' require 'pp' def names_match(entry1, entry2) if entry1.seq_name =~ /^([^ ]+) \d:/ name1 = $1 elsif entry1.seq_name =~ /^(.+)\/\d$/ name1 = $1 else raise "Could not match sequence name: #{entry1.seq_name}" end if entry2.seq_name =~ /^([^ ]+) \d:/ name2 = $1 elsif entry2.seq_name =~ /^(.+)\/\d$/ name2 = $1 else raise "Could not match sequence name: #{entry2.seq_name}" end name1 == name2 end casts = [] casts << {:long=>'mismatches', :short=>'m', :type=>'uint', :mandatory=>false, :default=>5, :allowed=>nil, :disallowed=>nil} casts << {:long=>'overlap_min', :short=>'o', :type=>'uint', :mandatory=>false, :default=>1, :allowed=>nil, :disallowed=>"0"} casts << {:long=>'overlap_max', :short=>'p', :type=>'uint', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>"0"} options = Biopieces.options_parse(ARGV, casts) entry1 = nil entry2 = nil Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record[:SEQ_NAME] and record[:SEQ] and record[:SCORES] if entry1.nil? entry1 = Seq.new_bp(record) elsif entry2.nil? entry2 = Seq.new_bp(record) end if entry1 and entry2 and entry1.length >= options[:overlap_min] and entry2.length >= options[:overlap_min] if names_match(entry1, entry2) entry2.type = :dna entry2.reverse!.complement! merged = Assemble.pair( entry1, entry2, mismatches_max:options[:mismatches], overlap_min:options[:overlap_min], overlap_max:options[:overlap_max] ) if merged new_record = merged.to_bp if merged.seq_name =~ /overlap=(\d+):hamming=(\d+)$/ new_record[:OVERLAP_LEN] = $1 new_record[:HAMMING_DIST] = $2 end output.puts new_record end else raise "name mismatch: #{entry1.seq_name} != #{entry2.seq_name}" end entry1 = nil entry2 = nil end else output.puts record end end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__