#!/usr/bin/env perl # Copyright (C) 2007-2009 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Analyze sequence tags in sequence or BED records from the stream. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Maasha::Biopieces; use Maasha::Seq; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $options, $in, $out, $record, %len_hash, %clone_hash, $clones, $key, $tag_record ); $options = Maasha::Biopieces::parse_options(); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { if ( $record->{ "SEQ_NAME" } and $record->{ "SEQ" } ) { if ( $record->{ "SEQ_NAME" } =~ /_(\d+)$/ ) { $clones = $1; $len_hash{ length( $record->{ "SEQ" } ) }++; $clone_hash{ length( $record->{ "SEQ" } ) } += $clones; } } elsif ( $record->{ "Q_ID" } and $record->{ "BED_LEN" } ) { if ( $record->{ "Q_ID" } =~ /_(\d+)$/ ) { $clones = $1; $len_hash{ $record->{ "BED_LEN" } }++; $clone_hash{ $record->{ "BED_LEN" } } += $clones; } } } foreach $key ( sort { $a <=> $b } keys %len_hash ) { $tag_record->{ "TAG_LEN" } = $key; $tag_record->{ "TAG_COUNT" } = $len_hash{ $key }; $tag_record->{ "TAG_CLONES" } = $clone_hash{ $key }; Maasha::Biopieces::put_record( $tag_record, $out ); } Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__