#!/usr/bin/env perl # Copyright (C) 2007-2009 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Write Biopiece records to the Biopieces Genome browser. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Data::Dumper; use Maasha::Common; use Maasha::KISS; use Maasha::Biopieces; use Maasha::Fasta; use Maasha::BGB::Track; use Maasha::BGB::Wiggle; use Maasha::Filesys; use constant { SEQ_NAME => 0, SEQ => 1, S_ID => 0, }; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $data_dir, $user, $options, $path, $in, $out, $tmp_dir, %fh_hash, $fh_out, $record, $entry, $key, $dst_dir, @nums, $num, $contig_dir, $wig ); $data_dir = Maasha::Biopieces::biopiecesrc( "BGB_DATA_DIR" ); $user = Maasha::Biopieces::biopiecesrc( "BGB_USER" ); $options = Maasha::Biopieces::parse_options( [ { long => 'user', short => 'u', type => 'string', mandatory => 'no', default => $user, allowed => undef, disallowed => undef }, { long => 'clade', short => 'c', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'genome', short => 'g', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'assembly', short => 'a', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'track_name', short => 't', type => 'string', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'track_type', short => 'T', type => 'string', mandatory => 'no', default => 'linear', allowed => 'linear,wiggle', disallowed => undef }, { long => 'force', short => 'f', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); if ( $options->{ 'track_name' } ) { $options->{ 'track_name' } =~ tr/ /_/; $path = join "/", $data_dir, "Users", $options->{ 'user' }, $options->{ 'clade' }, $options->{ 'genome' }, $options->{ 'assembly' }; Maasha::Common::error( qq(Path not found: "$path") ) if not -d $path; $tmp_dir = Maasha::Biopieces::get_tmpdir(); while ( $record = Maasha::Biopieces::get_record( $in ) ) { if ( $entry = Maasha::KISS::biopiece2kiss( $record ) ) { $entry->[ S_ID ] = ( split / /, $entry->[ S_ID ] )[ 0 ]; if ( not exists $fh_hash{ $entry->[ S_ID ] } ) { $fh_hash{ $entry->[ S_ID ] } = Maasha::Filesys::file_write_open( "$tmp_dir/$entry->[ S_ID ]" ); } $fh_out = $fh_hash{ $entry->[ S_ID ] }; Maasha::KISS::kiss_entry_put( $entry, $fh_out ); } Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" }; } $num = Maasha::BGB::Track::max_track( $options->{ 'user' }, $options->{ 'clade' }, $options->{ 'genome' }, $options->{ 'assembly' } ); $num = sprintf( "%04d", $num + 10 ); foreach $key ( keys %fh_hash ) { close $fh_hash{ $key }; $dst_dir = Maasha::Filesys::dir_create_if_not_exists( "$path/$key" ); $dst_dir = Maasha::Filesys::dir_create_if_not_exists( "$dst_dir/Tracks" ); $dst_dir = "$dst_dir/$num" . "_$options->{ 'track_name' }"; Maasha::Filesys::dir_create( $dst_dir ); if ( $options->{ 'track_type' } eq 'linear' ) { Maasha::Filesys::file_copy( "$tmp_dir/$key", "$dst_dir/track_data.kiss" ); Maasha::KISS::kiss_sort( "$dst_dir/track_data.kiss" ); } elsif ( $options->{ 'track_type' } eq 'wiggle' ) { $wig = Maasha::BGB::Wiggle::wiggle_encode( "$tmp_dir/$key" ); Maasha::BGB::Wiggle::wiggle_store( "$dst_dir/track_data.wig", $wig ); } unlink "$tmp_dir/$key"; } } else { $path = join "/", $data_dir, "Users/", $options->{ 'user' }, $options->{ 'clade' }; Maasha::Common::error( qq(Path not found: "$path") ) if not -d $path; $dst_dir = Maasha::Filesys::dir_create_if_not_exists( "$path/$options->{ 'genome' }" ); $dst_dir = Maasha::Filesys::dir_create( "$dst_dir/$options->{ 'assembly' }" ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { if ( $entry = Maasha::Fasta::biopiece2fasta( $record ) ) { $entry->[ SEQ_NAME ] = ( split " ", $entry->[ SEQ_NAME ] )[ 0 ]; if ( $options->{ 'force' } ) { Maasha::Filesys::dir_remove( "$dst_dir/$entry->[ SEQ_NAME ]" ); } $contig_dir = Maasha::Filesys::dir_create( "$dst_dir/$entry->[ SEQ_NAME ]" ); $contig_dir = Maasha::Filesys::dir_create( "$contig_dir/Sequence" ); $fh_out = Maasha::Filesys::file_write_open( "$contig_dir/sequence.txt" ); print $fh_out $entry->[ SEQ ]; close $fh_out; } } } Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__