#!/usr/bin/env perl # Copyright (C) 2007-2009 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Intersects two tracks in the Biopieces Genome Browser. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< use warnings; use strict; use Data::Dumper; use Maasha::Biopieces; use Maasha::BGB::Track; use Maasha::KISS; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< my ( $user, $options, $in, $out, $record, @contigs, $contig, $track, @tracks, %hash, $fh1, $fh2, $entries, $entry ); $user = Maasha::Biopieces::biopiecesrc( "BGB_USER" ); $options = Maasha::Biopieces::parse_options( [ { long => 'user', short => 'u', type => 'string', mandatory => 'no', default => $user, allowed => undef, disallowed => undef }, { long => 'clade', short => 'c', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'genome', short => 'g', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'assembly', short => 'a', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'track1', short => 't', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'track2', short => 'T', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, { long => 'invert', short => 'i', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, ] ); $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); while ( $record = Maasha::Biopieces::get_record( $in ) ) { Maasha::Biopieces::put_record( $record, $out ); } Maasha::Common::error( qq(Bad user: "$options->{ 'user' }") ) if not grep /^$options->{ 'user' }$/, Maasha::BGB::Track::list_users(); @contigs = Maasha::BGB::Track::list_contigs( $options->{ 'user' }, $options->{ 'clade' }, $options->{ 'genome' }, $options->{ 'assembly' } ); foreach $contig ( @contigs ) { undef %hash; @tracks = Maasha::BGB::Track::list_track_dir( $options->{ 'user' }, $options->{ 'clade' }, $options->{ 'genome' }, $options->{ 'assembly' }, $contig ); foreach $track ( @tracks ) { if ( index( $track, $options->{ 'track1' } ) >= 0 ) { $hash{ '1' } = $track; } elsif ( index( $track, $options->{ 'track2' } ) >= 0 ) { $hash{ '2' } = $track; } } if ( exists $hash{ '1' } and exists $hash{ '2' } ) { print STDERR "Intersecting:\n$hash{ '1' } and\n$hash{ '2' }\n\n" if $options->{ 'verbose' }; $fh1 = Maasha::Filesys::file_read_open( "$hash{ '1' }/track_data.kiss" ); $fh2 = Maasha::Filesys::file_read_open( "$hash{ '2' }/track_data.kiss" ); $entries = Maasha::KISS::kiss_intersect( $fh1, $fh2, $options->{ 'invert' } ); foreach $entry ( @{ $entries } ) { if ( $record = Maasha::KISS::kiss2biopiece( $entry ) ) { Maasha::Biopieces::put_record( $record, $out ); } } } } Maasha::Biopieces::close_stream( $in ); Maasha::Biopieces::close_stream( $out ); # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< BEGIN { Maasha::Biopieces::status_set(); } END { Maasha::Biopieces::status_log(); } # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__