From a8f2b8dbd9d579c56598b63a81cc6275ff4d6609 Mon Sep 17 00:00:00 2001 From: martinahansen Date: Thu, 11 Feb 2010 16:35:57 +0000 Subject: [PATCH] added option for database search to blat_seq git-svn-id: http://biopieces.googlecode.com/svn/trunk@875 74ccb610-7750-0410-82ae-013aeee3265d --- bp_bin/blat_seq | 34 +++++++++++++++++++++------------- 1 file changed, 21 insertions(+), 13 deletions(-) diff --git a/bp_bin/blat_seq b/bp_bin/blat_seq index 220e7b3..073cfd8 100755 --- a/bp_bin/blat_seq +++ b/bp_bin/blat_seq @@ -38,27 +38,35 @@ use Maasha::UCSC::PSL; # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< -my ( $options, $in, $out, $record, $blat_args, $genome_file, $query_file, $fh_in, $fh_out, +my ( $options, $in, $out, $record, $blat_args, $subject_file, $query_file, $fh_in, $fh_out, $tmp_dir, $type, $result_file, $entry ); $options = Maasha::Biopieces::parse_options( [ - { long => 'genome', short => 'g', type => 'genome', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef }, - { long => 'fast_map', short => 'f', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, - { long => 'occ', short => 'c', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, - { long => 'intron_max', short => 'i', type => 'uint', mandatory => 'no', default => 750000, allowed => undef, disallowed => undef }, - { long => 'tile_size', short => 't', type => 'uint', mandatory => 'no', default => 11, allowed => undef, disallowed => 0 }, - { long => 'step_size', short => 's', type => 'uint', mandatory => 'no', default => 11, allowed => undef, disallowed => 0 }, - { long => 'min_identity', short => 'm', type => 'uint', mandatory => 'no', default => 90, allowed => undef, disallowed => 0 }, - { long => 'min_score', short => 'M', type => 'uint', mandatory => 'no', default => 0, allowed => undef, disallowed => undef }, - { long => 'one_off', short => 'o', type => 'uint', mandatory => 'no', default => 0, allowed => undef, disallowed => undef }, + { long => 'database', short => 'd', type => 'file', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + { long => 'genome', short => 'g', type => 'genome', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + { long => 'fast_map', short => 'f', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + { long => 'occ', short => 'c', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + { long => 'intron_max', short => 'i', type => 'uint', mandatory => 'no', default => 750000, allowed => undef, disallowed => undef }, + { long => 'tile_size', short => 't', type => 'uint', mandatory => 'no', default => 11, allowed => undef, disallowed => 0 }, + { long => 'step_size', short => 's', type => 'uint', mandatory => 'no', default => 11, allowed => undef, disallowed => 0 }, + { long => 'min_identity', short => 'm', type => 'uint', mandatory => 'no', default => 90, allowed => undef, disallowed => 0 }, + { long => 'min_score', short => 'M', type => 'uint', mandatory => 'no', default => 0, allowed => undef, disallowed => undef }, + { long => 'one_off', short => 'o', type => 'uint', mandatory => 'no', default => 0, allowed => undef, disallowed => undef }, ] ); +Maasha::Common::error( qq(both --database and --genome specified) ) if $options->{ "genome" } and $options->{ "database" }; +Maasha::Common::error( qq(no --database or --genome specified) ) if not $options->{ "genome" } and not $options->{ "database" }; + $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); -$genome_file = "$ENV{ 'BP_DATA' }/genomes/$options->{ 'genome' }/fasta/$options->{ 'genome' }.fna"; +if ( $options->{ 'database' } ) { + $subject_file = $options->{ 'database' }; +} else { + $subject_file = "$ENV{ 'BP_DATA' }/genomes/$options->{ 'genome' }/fasta/$options->{ 'genome' }.fna"; +} $blat_args .= " -tileSize=$options->{ 'tile_size' }"; $blat_args .= " -oneOff=$options->{ 'one_off' }"; @@ -93,9 +101,9 @@ $blat_args .= " -q=$type"; $result_file = "$tmp_dir/blat.psl"; if ( $options->{ 'verbose' } ) { - Maasha::Common::run( "blat", "$genome_file $query_file $blat_args $result_file" ); + Maasha::Common::run( "blat", "$subject_file $query_file $blat_args $result_file" ); } else { - Maasha::Common::run( "blat", "$genome_file $query_file $blat_args $result_file > /dev/null 2>&1" ); + Maasha::Common::run( "blat", "$subject_file $query_file $blat_args $result_file > /dev/null 2>&1" ); } unlink $query_file; -- 2.39.5