From 8c6684ff7f9c56aa0b92aa075010524043f19151 Mon Sep 17 00:00:00 2001 From: martinahansen Date: Wed, 31 Oct 2012 20:50:00 +0000 Subject: [PATCH] added order_pairs2 git-svn-id: http://biopieces.googlecode.com/svn/trunk@1976 74ccb610-7750-0410-82ae-013aeee3265d --- bp_bin/order_pairs2 | 105 ++++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 105 insertions(+) create mode 100755 bp_bin/order_pairs2 diff --git a/bp_bin/order_pairs2 b/bp_bin/order_pairs2 new file mode 100755 index 0000000..3485460 --- /dev/null +++ b/bp_bin/order_pairs2 @@ -0,0 +1,105 @@ +#!/usr/bin/env ruby + +# Copyright (C) 2007-2012 Martin A. Hansen. + +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. + +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. + +# You should have received a copy of the GNU General Public License +# along with this program; if not, write to the Free Software +# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. + +# http://www.gnu.org/copyleft/gpl.html + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# This program is part of the Biopieces framework (www.biopieces.org). + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# Order records with pair end sequence data. + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +require 'maasha/biopieces' +require 'dbm' +require 'pp' + +ILLUMINA15 = 15 +ILLUMINA18 = 18 + +options = Biopieces.options_parse(ARGV) + +tmpdir = Biopieces.mktmpdir +tmpdir = "Sletmig" +tmpfile = File.join(tmpdir, "data") + +db = DBM.new(tmpfile, 666, DBM::NEWDB) + +Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| + input.each_record do |record| + if record[:SEQ_NAME] + db[record[:SEQ_NAME]] = Marshal.dump(record) + else + output.puts record + end + end + + skip = {} + + db.keys.each do |seq_name| + next if skip[seq_name] + + case seq_name + when /^(.+)\/(\d)$/ # Illumina 1.5 + type = ILLUMINA15 + name = $1 + pair = $2.to_i + when /^(.+) (\d):/ # Illumina 1.8 + type = ILLUMINA18 + name = $1.to_sym + pair = $2.to_i + else + $stderr.puts "WARNING: Unmatched sequence name: #{record[:SEQ_NAME]}" + end + + pair2 = (pair == 1) ? 2 : 1 + + if type == ILLUMINA15 + seq_name2 = "#{name}/#{pair2}" + else + seq_name2 = seq_name.sub(/^(.+) \d:/, "#{$1} #{pair2}:") + end + + record1 = Marshal.load(db[seq_name]) + record2 = db[seq_name2] + record2 = Marshal.load(record2) unless record2.nil? + + if record2.nil? + record1[:ORDER] = "orphan #{pair}" + output.puts record1 + else + record1[:ORDER] = "paired" + record2[:ORDER] = "paired" + + output.puts record1 + output.puts record2 + + skip[seq_name2] = true + end + end +end + +File.delete(tmpfile + ".db") + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +__END__ -- 2.39.5