From 6dce31d9c50a6e0565f816547c86009486ebf2ef Mon Sep 17 00:00:00 2001 From: martinahansen Date: Wed, 18 Apr 2012 08:10:47 +0000 Subject: [PATCH] added findsim_seq biopieces git-svn-id: http://biopieces.googlecode.com/svn/trunk@1799 74ccb610-7750-0410-82ae-013aeee3265d --- bp_bin/findsim_seq | 74 ++++++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 74 insertions(+) create mode 100755 bp_bin/findsim_seq diff --git a/bp_bin/findsim_seq b/bp_bin/findsim_seq new file mode 100755 index 0000000..d6879cc --- /dev/null +++ b/bp_bin/findsim_seq @@ -0,0 +1,74 @@ +#!/usr/bin/env ruby + +# Copyright (C) 2007-2012 Martin A. Hansen. + +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. + +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. + +# You should have received a copy of the GNU General Public License +# along with this program; if not, write to the Free Software +# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. + +# http://www.gnu.org/copyleft/gpl.html + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# This program is part of the Biopieces framework (www.biopieces.org). + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# Find similar sequences between query sequences from the stream and a database. + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +require 'pp' +require 'maasha/biopieces' +require 'maasha/fasta' +require 'maasha/findsim' + +casts = [] +casts << {:long=>'database', :short=>'d', :type=>'file!', :mandatory=>true, :default=>nil, :allowed=>nil, :disallowed=>nil} +casts << {:long=>'kmer', :short=>'k', :type=>'uint', :mandatory=>false, :default=>8, :allowed=>"4,5,6,7,8,9", :disallowed=>nil} +casts << {:long=>'step', :short=>'s', :type=>'uint', :mandatory=>false, :default=>1, :allowed=>nil, :disallowed=>"0"} +casts << {:long=>'report_scores', :short=>'r', :type=>'uint', :mandatory=>false, :default=>7, :allowed=>nil, :disallowed=>"0"} +casts << {:long=>'min_score', :short=>'m', :type=>'float', :mandatory=>false, :default=>0.5, :allowed=>nil, :disallowed=>nil} +casts << {:long=>'query_ids', :short=>'Q', :type=>'flag', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} +casts << {:long=>'subject_ids', :short=>'S', :type=>'flag', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} + +options = Biopieces.options_parse(ARGV, casts) +tmpdir = Biopieces.mktmpdir +query_file = File.join(tmpdir, "query.fna") + +Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| + Fasta.open(query_file, "w") do |fasta_io| + input.each_record do |record| + if record[:SEQ_NAME] and record[:SEQ] + entry = Seq.new_bp(record) + + fasta_io.puts entry.to_fasta + end + + output.puts record + end + end + + fs = FindSim.new(options) + fs.load_query(query_file) + fs.search_db(options[:database]) + + fs.each do |hit| + output.puts hit.to_bp + end +end + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +__END__ -- 2.39.5