From 004fa0ccbda503d45e8af18758706c261c13337a Mon Sep 17 00:00:00 2001 From: martinahansen Date: Thu, 1 Sep 2011 13:36:19 +0000 Subject: [PATCH] added find_SNPs biopiece git-svn-id: http://biopieces.googlecode.com/svn/trunk@1508 74ccb610-7750-0410-82ae-013aeee3265d --- bp_bin/find_SNPs | 91 ++++++++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 91 insertions(+) create mode 100755 bp_bin/find_SNPs diff --git a/bp_bin/find_SNPs b/bp_bin/find_SNPs new file mode 100755 index 0000000..6671144 --- /dev/null +++ b/bp_bin/find_SNPs @@ -0,0 +1,91 @@ +#!/usr/bin/env ruby + +# Copyright (C) 2007-2011 Martin A. Hansen. + +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. + +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. + +# You should have received a copy of the GNU General Public License +# along with this program; if not, write to the Free Software +# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. + +# http://www.gnu.org/copyleft/gpl.html + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# This program is part of the Biopieces framework (www.biopieces.org). + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# Find SNPs in records of SAM type in the stream. + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +require 'maasha/biopieces' +require 'pp' + +options = Biopieces.options_parse(ARGV) + +snp_hash = Hash.new { |h,k| h[k] = Hash.new { |h,k| h[k] = Hash.new(0) } } + +Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| + input.each_record do |record| + output.puts record + + if record.has_key? :ALIGN and + record.has_key? :S_ID and + record.has_key? :S_BEG and + record[:ALIGN] != '.' + + record[:ALIGN].split(',').each do |snp| + pos, event = snp.split(':') + + pos += record[:S_BEG] + + snp_hash[record[:S_ID].to_sym][pos.to_sym][event.to_sym] += 1 + end + end + end + + snp_hash.each_pair do |s_id, pos_hash| + new_record = {} + new_record[:REC_TYPE] = 'SNP' + new_record[:S_ID] = s_id + + pos_hash.each_pair do |pos, event_hash| + new_record[:POS] = pos + + event_hash.each_pair do |event, count| + new_record[:EVENT] = event + new_record[:SNP_COUNT] = count + + before, after = event.to_s.split('>') + + if before == '-' + type = 'INSERTION' + elsif after == '-' + type = 'DELETION' + else + type = 'MISMATCH' + end + new_record[:TYPE] = type + end + + output.puts new_record + end + end +end + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +__END__ -- 2.39.5