From: martinahansen <martinahansen@74ccb610-7750-0410-82ae-013aeee3265d>
Date: Fri, 14 Aug 2009 16:35:06 +0000 (+0000)
Subject: added rescan_seq Restriction Enzyme scanner
X-Git-Url: https://git.donarmstrong.com/?a=commitdiff_plain;h=cdd1aa98d4e29baa49ed44a28d6e16d3fb77f49a;p=biopieces.git

added rescan_seq Restriction Enzyme scanner

git-svn-id: http://biopieces.googlecode.com/svn/trunk@636 74ccb610-7750-0410-82ae-013aeee3265d
---

diff --git a/bp_bin/rescan_seq b/bp_bin/rescan_seq
new file mode 100755
index 0000000..22e0596
--- /dev/null
+++ b/bp_bin/rescan_seq
@@ -0,0 +1,106 @@
+#!/usr/bin/env perl
+
+# Copyright (C) 2007-2009 Martin A. Hansen.
+
+# This program is free software; you can redistribute it and/or
+# modify it under the terms of the GNU General Public License
+# as published by the Free Software Foundation; either version 2
+# of the License, or (at your option) any later version.
+
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+# GNU General Public License for more details.
+
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
+
+# http://www.gnu.org/copyleft/gpl.html
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+# Scan sequences in the stream for restriction enzyme cleavage sites.
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+use warnings;
+use strict;
+use Data::Dumper;
+use Maasha::RestrictEnz;
+use Maasha::Biopieces;
+use Maasha::Patscan;
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+my ( $options, $in, $out, $record, $re_data, $re, $matches, $match, %re_hash, $res_enz );
+
+$options = Maasha::Biopieces::parse_options(
+    [
+        { long => 'res_enz',    short => 'r', type => 'list',  mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
+        { long => 'res_enz_in', short => 'R', type => 'file!', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
+        { long => 'no_matches', short => 'M', type => 'flag',  mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
+    ]   
+);
+
+$in  = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
+$out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } );
+
+$re_data = Maasha::RestrictEnz::parse_re_data();
+
+if ( $options->{ 'res_enz_in' } ) {
+    $res_enz = Maasha::Patscan::read_patterns( $options->{ "res_enz_in" } );
+}
+
+push @{ $res_enz }, @{ $options->{ 'res_enz' } } if defined $options->{ 'res_enz' };
+
+map { $re_hash{ $_ } = 1 } @{ $res_enz };
+
+while ( $record = Maasha::Biopieces::get_record( $in ) ) 
+{
+    if ( $record->{ 'SEQ' } )
+    {
+        foreach $re ( @{ $re_data } )
+        {
+            next if not exists $re_hash{ $re->{ 'name' } };
+
+            $matches = Maasha::RestrictEnz::re_scan( $record->{ 'SEQ' }, $re );
+
+            if ( scalar @{ $matches } > 0 )
+            {
+                $record->{ $re->{ 'name' } . "_COUNT" }   = scalar @{ $matches };
+                $record->{ $re->{ 'name' } . "_MATCHES" } = join( ";", @{ $matches } ) if not $options->{ 'no_matches' };
+            }
+        }
+    }
+
+    Maasha::Biopieces::put_record( $record, $out );
+}
+
+Maasha::Biopieces::close_stream( $in );
+Maasha::Biopieces::close_stream( $out );
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+BEGIN
+{
+    Maasha::Biopieces::status_set();
+}
+
+
+END
+{
+    Maasha::Biopieces::status_log();
+}
+
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+__END__