From: martinahansen <martinahansen@74ccb610-7750-0410-82ae-013aeee3265d>
Date: Thu, 17 Nov 2011 14:16:16 +0000 (+0000)
Subject: added remove_primers biopiece
X-Git-Url: https://git.donarmstrong.com/?a=commitdiff_plain;h=95478f36bede26ea54caf840659ec94654c99c95;p=biopieces.git

added remove_primers biopiece

git-svn-id: http://biopieces.googlecode.com/svn/trunk@1658 74ccb610-7750-0410-82ae-013aeee3265d
---

diff --git a/bp_bin/remove_primers b/bp_bin/remove_primers
new file mode 100755
index 0000000..a68fb89
--- /dev/null
+++ b/bp_bin/remove_primers
@@ -0,0 +1,78 @@
+#!/usr/bin/env ruby
+
+# Copyright (C) 2007-2011 Martin A. Hansen.
+
+# This program is free software; you can redistribute it and/or
+# modify it under the terms of the GNU General Public License
+# as published by the Free Software Foundation; either version 2
+# of the License, or (at your option) any later version.
+
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+# GNU General Public License for more details.
+
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
+
+# http://www.gnu.org/copyleft/gpl.html
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+# This program is part of the Biopieces framework (www.biopieces.org).
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+# Remove primers from sequences in the stream.
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+
+require 'pp'
+require 'maasha/biopieces'
+require 'maasha/seq'
+
+casts = []
+casts << {:long=>'forward',    :short=>'f', :type=>'string', :mandatory=>true,  :default=>nil, :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'reverse',    :short=>'r', :type=>'string', :mandatory=>true,  :default=>nil, :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'mismatches', :short=>'m', :type=>'uint',   :mandatory=>false, :default=>2,   :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'insertions', :short=>'i', :type=>'uint',   :mandatory=>false, :default=>1,   :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'deletions',  :short=>'d', :type=>'uint',   :mandatory=>false, :default=>1,   :allowed=>nil, :disallowed=>nil}
+
+options = Biopieces.options_parse(ARGV, casts)
+
+Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output|
+  input.each do |record|
+    if record.has_key? :SEQ
+      seq = Seq.new_bp(record)
+      pos = 0
+
+      if forward = seq.patscan(options[:forward].to_s, pos, options[:mismatches], options[:insertions], options[:deletions])
+        record[:FORWARD_POS] = forward.last.pos
+        record[:FORWARD_LEN] = forward.last.length
+        pos = forward.last.pos + forward.last.length
+        seq.subseq!(pos)
+      end
+
+      if reverse = seq.patscan(options[:reverse].to_s, pos, options[:mismatches], options[:insertions], options[:deletions])
+        record[:REVERSE_POS] = reverse.last.pos
+        record[:REVERSE_LEN] = reverse.last.length
+        pos = reverse.last.pos
+        seq.subseq!(pos)
+      end
+
+      if pos > 0
+        record.merge!(seq.to_bp)
+      end
+
+      output.puts record
+    else
+      output.puts record
+    end
+  end
+end
+
+# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
+
+__END__