From: martinahansen Date: Tue, 15 Dec 2009 11:28:31 +0000 (+0000) Subject: added find_genes X-Git-Url: https://git.donarmstrong.com/?a=commitdiff_plain;h=2de7ef3aa2b6bca76057b2c527169ead62aa79f3;p=biopieces.git added find_genes git-svn-id: http://biopieces.googlecode.com/svn/trunk@817 74ccb610-7750-0410-82ae-013aeee3265d --- diff --git a/bp_bin/find_genes b/bp_bin/find_genes new file mode 100755 index 0000000..f80fa88 --- /dev/null +++ b/bp_bin/find_genes @@ -0,0 +1,143 @@ +#!/usr/bin/env perl + +# Copyright (C) 2007-2009 Martin A. Hansen. + +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. + +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. + +# You should have received a copy of the GNU General Public License +# along with this program; if not, write to the Free Software +# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. + +# http://www.gnu.org/copyleft/gpl.html + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# Find genes in sequences in stream. + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +use warnings; +use strict; +use Data::Dumper; +use Maasha::Common; +use Maasha::Biopieces; +use Maasha::Filesys; + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +my ( $options, $in, $out, $record, $tmp_dir, $tmp_file, $fh_out, $fh_in, $entry, $chunk, @lines, $line, $s_id, $type, $s_beg, $s_end, $strand, @fields ); + +$options = Maasha::Biopieces::parse_options(); + +$in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); +$out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); + +$tmp_dir = Maasha::Biopieces::get_tmpdir(); +$tmp_file = "$tmp_dir/tmp.seq"; +$fh_out = Maasha::Filesys::file_write_open( $tmp_file ); + +while ( $record = Maasha::Biopieces::get_record( $in ) ) +{ + if ( $entry = Maasha::Fasta::biopiece2fasta( $record ) ) { + Maasha::Fasta::put_entry( $entry, $fh_out ); + } + + Maasha::Biopieces::put_record( $record, $out ); +} + +Maasha::Common::run( "draft_prodigal.pl", "< $tmp_file > $tmp_file.out 2> /dev/null" ); + +$fh_in = Maasha::Filesys::file_read_open( "$tmp_file.out" ); + +$/ = "//\n"; + +while ( $chunk = <$fh_in> ) +{ + chomp $chunk; + + @lines = split /\n/, $chunk; + + $line = shift @lines; + + if ( $line =~ /.*>(.+)/ ) + { + $s_id = $1; + + foreach $line ( @lines ) + { + @fields = split " ", $line; + + $type = $fields[ 0 ]; + + if ( $fields[ 1 ] =~ /complement/ ) { + $strand = "-"; + } else { + $strand = "+"; + } + + if ( $fields[ 1 ] =~ /(\d+)\.\.>?(\d+)/ ) + { + $s_beg = $1; + $s_end = $2; + } + else + { + Maasha::Common::error( qq(BAD locator: $line) ); + } + + $record = { + S_ID => $s_id, + S_BEG => $s_beg - 1, + S_END => $s_end - 1, + Q_ID => $type, + STRAND => $strand, + }; + + Maasha::Biopieces::put_record( $record, $out ); + } + } + else + { + Maasha::Commom::error( qq(BAD definition: $line) ); + } +} + +close $fh_in; + +$/ = "\n"; + +Maasha::Biopieces::close_stream( $in ); +Maasha::Biopieces::close_stream( $out ); + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +BEGIN +{ + Maasha::Biopieces::status_set(); +} + + +END +{ + Maasha::Biopieces::status_log(); +} + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +__END__