From: martinahansen Date: Fri, 14 Aug 2009 15:37:38 +0000 (+0000) Subject: added RestrictEnz.pm X-Git-Url: https://git.donarmstrong.com/?a=commitdiff_plain;h=1874b8942d462bf445664010754066a3f3d9ee0c;p=biopieces.git added RestrictEnz.pm git-svn-id: http://biopieces.googlecode.com/svn/trunk@634 74ccb610-7750-0410-82ae-013aeee3265d --- diff --git a/code_perl/Maasha/RestrictEnz.pm b/code_perl/Maasha/RestrictEnz.pm new file mode 100644 index 0000000..9b1dd27 --- /dev/null +++ b/code_perl/Maasha/RestrictEnz.pm @@ -0,0 +1,1008 @@ +package Maasha::RestrictEnz; + + +# Copyright (C) 2006-2007 Martin A. Hansen. + +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. + +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. + +# You should have received a copy of the GNU General Public License +# along with this program; if not, write to the Free Software +# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. + +# http://www.gnu.org/copyleft/gpl.html + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +# This module contains routines for matching restriction enzyme cleavage sites. + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +use warnings; +use strict; +use Data::Dumper; + +use Exporter; + +use vars qw( @ISA @EXPORT @EXPORT_OK ); + +@ISA = qw( Exporter ) ; + +use Inline ( C => <<'END_C', DIRECTORY => $ENV{ "BP_TMP" } ); + +/* +# UIPAC ambiguity codes for nucleotides: +# +# http://droog.gs.washington.edu/parc/images/iupac.html +# +# ACGTUMRWSYKVHDBN +# A1000011100011101 +# C0100010011011011 +# G0010001010110111 +# T0001100101101111 +# U0001100101101111 +# M1100000000000000 +# R1010000000000000 +# W1001100000000000 +# S0110000000000000 +# Y0101100000000000 +# K0011100000000000 +# V1110000000000000 +# H1101100000000000 +# D1011100000000000 +# B0111100000000000 +# N1111100000000000 +*/ + + +char ambi_match[16][16] = { + "1000011100011101", + "0100010011011011", + "0010001010110111", + "0001100101101111", + "0001100101101111", + "1100000000000000", + "1010000000000000", + "1001100000000000", + "0110000000000000", + "0101100000000000", + "0011100000000000", + "1110000000000000", + "1101100000000000", + "1011100000000000", + "0111100000000000", + "1111100000000000" +}; + + +int hash( char c ) +{ + switch ( toupper( c ) ) + { + case 'A': return 0; + case 'C': return 1; + case 'G': return 2; + case 'T': return 3; + case 'U': return 4; + case 'M': return 5; + case 'R': return 6; + case 'W': return 7; + case 'S': return 8; + case 'Y': return 9; + case 'K': return 10; + case 'V': return 11; + case 'H': return 12; + case 'D': return 13; + case 'B': return 14; + case 'N': return 0; + default: return -1; + } +} + + +void scan( char *seq, char *pat, int seq_len, int pat_len ) +{ + int i; + + Inline_Stack_Vars; + Inline_Stack_Reset; + + for ( i = 0; i < seq_len - pat_len; i++ ) + { + if ( match( &seq[ i ], pat, pat_len ) ) { + Inline_Stack_Push( sv_2mortal( newSViv( i ) ) ); + } + } + + Inline_Stack_Done; +} + + +int match( char *seq1, char *seq2, int len ) +{ + int i = 0; + char c1; + char c2; + + while ( i < len ) + { + c1 = seq1[ i ]; + c2 = seq2[ i ]; + + if ( ambi_match[ hash( c1 ) ][ hash( c2 ) ] == '0' ) { + return 0; + } + + i++; + } + + return 1; +} + +END_C + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +sub re_scan +{ + my ( $seq, # sequence to scan + $re, # hashref with RE info + ) = @_; + + my ( @matches ); + + @matches = scan( $seq, $re->{ "pattern" }, length $seq, $re->{ "len" } ); + + return wantarray ? @matches: \@matches; +} + + +sub parse_re_data +{ + my ( @lines, $line, @fields, @re_data ); + + @lines = ; + + chomp @lines; + + foreach $line ( @lines ) + { + next if $line =~ /^(#|$)/; + + @fields = split " ", $line; + + push @re_data, { + name => $fields[ 0 ], + pattern => $fields[ 1 ], + len => $fields[ 2 ], + ncuts => $fields[ 3 ], + blunt => $fields[ 4 ], + c1 => $fields[ 5 ], + c2 => $fields[ 6 ], + c3 => $fields[ 7 ], + c4 => $fields[ 8 ], + }; + } + + return wantarray ? @re_data : \@re_data; +} + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +__DATA__ + +# From REBASE: +# ftp://ftp.neb.com/pub/rebase/emboss_e.908 + +# REBASE version 908 emboss_e.908 +# +# =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= +# REBASE, The Restriction Enzyme Database http://rebase.neb.com +# Copyright (c) Dr. Richard J. Roberts, 2009. All rights reserved. +# =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= +# +# Rich Roberts Jul 28 2009 +# +# REBASE enzyme patterns for EMBOSS (embossre.enz) +# +# Format: +# +# namepatternlenncutsbluntc1c2c3c4 +# +# Where: +# name = name of enzyme +# pattern = recognition site +# len = length of pattern +# ncuts = number of cuts made by enzyme +# Zero represents unknown +# blunt = true if blunt end cut, false if sticky +# c1 = First 5' cut +# c2 = First 3' cut +# c3 = Second 5' cut +# c4 = Second 3' cut +# +# Examples: +# AAC^TGG -> 6 2 1 3 3 0 0 +# A^ACTGG -> 6 2 0 1 5 0 0 +# AACTGG -> 6 0 0 0 0 0 0 +# AACTGG(-5/-1) -> 6 2 0 1 5 0 0 +# (8/13)GACNNNNNNTCA(12/7) -> 12 4 0 -9 -14 24 19 +# +# i.e. cuts are always to the right of the given +# residue and sequences are always with reference to +# the 5' strand. +# Sequences are numbered ... -3 -2 -1 1 2 3 ... with +# the first residue of the pattern at base number 1. +# +# + +AanI TTATAA 6 2 1 3 3 0 0 +AarI CACCTGC 7 2 0 11 15 0 0 +AasI GACNNNNNNGTC 12 2 0 7 5 0 0 +AatI AGGCCT 6 2 1 3 3 0 0 +AatII GACGTC 6 2 0 5 1 0 0 +AbsI CCTCGAGG 8 2 0 2 6 0 0 +AccI GTMKAC 6 2 0 2 4 0 0 +AccII CGCG 4 2 1 2 2 0 0 +AccIII TCCGGA 6 2 0 1 5 0 0 +Acc16I TGCGCA 6 2 1 3 3 0 0 +Acc36I ACCTGC 6 2 0 10 14 0 0 +Acc65I GGTACC 6 2 0 1 5 0 0 +AccB1I GGYRCC 6 2 0 1 5 0 0 +AccB7I CCANNNNNTGG 11 2 0 7 4 0 0 +AccBSI CCGCTC 6 2 1 3 3 0 0 +AceIII cagctc 6 2 0 13 17 0 0 +AciI CCGC 4 2 0 1 3 0 0 +AclI AACGTT 6 2 0 2 4 0 0 +AclWI GGATC 5 2 0 9 10 0 0 +AcoI YGGCCR 6 2 0 1 5 0 0 +AcsI RAATTY 6 2 0 1 5 0 0 +AcuI CTGAAG 6 2 0 22 20 0 0 +AcvI CACGTG 6 2 1 3 3 0 0 +AcyI GRCGYC 6 2 0 2 4 0 0 +AdeI CACNNNGTG 9 2 0 6 3 0 0 +AfaI GTAC 4 2 1 2 2 0 0 +AfeI AGCGCT 6 2 1 3 3 0 0 +AfiI CCNNNNNNNGG 11 2 0 7 4 0 0 +AflII CTTAAG 6 2 0 1 5 0 0 +AflIII ACRYGT 6 2 0 1 5 0 0 +AgeI ACCGGT 6 2 0 1 5 0 0 +AgsI TTSAA 5 2 0 3 2 0 0 +AhaIII tttaaa 6 2 1 3 3 0 0 +AhdI GACNNNNNGTC 11 2 0 6 5 0 0 +AhlI ACTAGT 6 2 0 1 5 0 0 +AjiI CACGTC 6 2 1 3 3 0 0 +AjnI CCWGG 5 2 0 -1 5 0 0 +AjuI GAANNNNNNNTTGG 14 4 0 -8 -13 25 20 +AleI CACNNNNGTG 10 2 1 5 5 0 0 +AlfI GCANNNNNNTGC 12 4 0 -11 -13 24 22 +AloI GAACNNNNNNTCC 13 4 0 -8 -13 25 20 +AluI AGCT 4 2 1 2 2 0 0 +AluBI AGCT 4 2 1 2 2 0 0 +AlwI GGATC 5 2 0 9 10 0 0 +Alw21I GWGCWC 6 2 0 5 1 0 0 +Alw26I GTCTC 5 2 0 6 10 0 0 +Alw44I GTGCAC 6 2 0 1 5 0 0 +AlwFI gaaaynnnnnrtg 13 0 0 0 0 0 0 +AlwNI CAGNNNCTG 9 2 0 6 3 0 0 +Ama87I CYCGRG 6 2 0 1 5 0 0 +Aor13HI TCCGGA 6 2 0 1 5 0 0 +Aor51HI AGCGCT 6 2 1 3 3 0 0 +ApaI GGGCCC 6 2 0 5 1 0 0 +ApaBI gcannnnntgc 11 2 0 8 3 0 0 +ApaLI GTGCAC 6 2 0 1 5 0 0 +ApeKI GCWGC 5 2 0 1 4 0 0 +ApoI RAATTY 6 2 0 1 5 0 0 +ApyPI atcgac 6 2 0 26 24 0 0 +AquIII gaggag 6 2 0 26 24 0 0 +AquIV grggaag 7 2 0 26 24 0 0 +ArsI GACNNNNNNTTYG 13 4 0 -9 -14 24 19 +AscI GGCGCGCC 8 2 0 2 6 0 0 +AseI ATTAAT 6 2 0 2 4 0 0 +Asi256I gatc 4 2 0 1 3 0 0 +AsiGI ACCGGT 6 2 0 1 5 0 0 +AsiSI GCGATCGC 8 2 0 5 3 0 0 +AspI GACNNNGTC 9 2 0 4 5 0 0 +Asp700I GAANNNNTTC 10 2 1 5 5 0 0 +Asp718I GGTACC 6 2 0 1 5 0 0 +AspA2I CCTAGG 6 2 0 1 5 0 0 +AspCNI gccgc 5 0 0 0 0 0 0 +AspEI GACNNNNNGTC 11 2 0 6 5 0 0 +AspLEI GCGC 4 2 0 3 1 0 0 +AspS9I GGNCC 5 2 0 1 4 0 0 +AssI AGTACT 6 2 1 3 3 0 0 +AsuI ggncc 5 2 0 1 4 0 0 +AsuII TTCGAA 6 2 0 2 4 0 0 +AsuC2I CCSGG 5 2 0 2 3 0 0 +AsuHPI GGTGA 5 2 0 13 12 0 0 +AsuNHI GCTAGC 6 2 0 1 5 0 0 +AvaI CYCGRG 6 2 0 1 5 0 0 +AvaII GGWCC 5 2 0 1 4 0 0 +AvaIII atgcat 6 0 0 0 0 0 0 +AviII TGCGCA 6 2 1 3 3 0 0 +AvrII CCTAGG 6 2 0 1 5 0 0 +AxyI CCTNAGG 7 2 0 2 5 0 0 +BaeI ACNNNNGTAYC 11 4 0 -11 -16 23 18 +BaeGI GKGCMC 6 2 0 5 1 0 0 +BalI TGGCCA 6 2 1 3 3 0 0 +BamHI GGATCC 6 2 0 1 5 0 0 +BanI GGYRCC 6 2 0 1 5 0 0 +BanII GRGCYC 6 2 0 5 1 0 0 +BanIII ATCGAT 6 2 0 2 4 0 0 +BarI GAAGNNNNNNTAC 13 4 0 -8 -13 25 20 +BasI CCANNNNNTGG 11 2 0 7 4 0 0 +BauI CACGAG 6 2 0 1 5 0 0 +BbeI GGCGCC 6 2 0 5 1 0 0 +Bbr7I gaagac 6 2 0 13 17 0 0 +BbrPI CACGTG 6 2 1 3 3 0 0 +BbsI GAAGAC 6 2 0 8 12 0 0 +BbuI GCATGC 6 2 0 5 1 0 0 +BbvI GCAGC 5 2 0 13 17 0 0 +BbvII gaagac 6 2 0 8 12 0 0 +Bbv12I GWGCWC 6 2 0 5 1 0 0 +BbvCI CCTCAGC 7 2 0 2 5 0 0 +BccI CCATC 5 2 0 9 10 0 0 +Bce83I cttgag 6 2 0 22 20 0 0 +BceAI ACGGC 5 2 0 17 19 0 0 +BcefI acggc 5 2 0 17 18 0 0 +BcgI CGANNNNNNTGC 12 4 0 -11 -13 24 22 +BciVI GTATCC 6 2 0 12 11 0 0 +BclI TGATCA 6 2 0 1 5 0 0 +BcnI CCSGG 5 2 0 2 3 0 0 +BcuI ACTAGT 6 2 0 1 5 0 0 +BdaI TGANNNNNNTCA 12 4 0 -11 -13 24 22 +BetI wccggw 6 2 0 1 5 0 0 +BfaI CTAG 4 2 0 1 3 0 0 +BfiI ACTGGG 6 2 0 11 10 0 0 +BfmI CTRYAG 6 2 0 1 5 0 0 +BfoI RGCGCY 6 2 0 5 1 0 0 +BfrI CTTAAG 6 2 0 1 5 0 0 +BfuI GTATCC 6 2 0 12 11 0 0 +BfuAI ACCTGC 6 2 0 10 14 0 0 +BfuCI GATC 4 2 0 -1 4 0 0 +BglI GCCNNNNNGGC 11 2 0 7 4 0 0 +BglII AGATCT 6 2 0 1 5 0 0 +BinI ggatc 5 2 0 9 10 0 0 +BisI GCNGC 5 2 0 2 3 0 0 +BlnI CCTAGG 6 2 0 1 5 0 0 +BlpI GCTNAGC 7 2 0 2 5 0 0 +BlsI GCNGC 5 2 0 3 2 0 0 +BmcAI AGTACT 6 2 1 3 3 0 0 +Bme18I GGWCC 5 2 0 1 4 0 0 +Bme1390I CCNGG 5 2 0 2 3 0 0 +BmeRI GACNNNNNGTC 11 2 0 6 5 0 0 +BmeT110I CYCGRG 6 2 0 1 5 0 0 +BmgI gkgccc 6 0 0 0 0 0 0 +BmgBI CACGTC 6 2 1 3 3 0 0 +BmgT120I GGNCC 5 2 0 2 3 0 0 +BmiI GGNNCC 6 2 1 3 3 0 0 +BmrI ACTGGG 6 2 0 11 10 0 0 +BmrFI CCNGG 5 2 0 2 3 0 0 +BmsI GCATC 5 2 0 10 14 0 0 +BmtI GCTAGC 6 2 0 5 1 0 0 +BmuI ACTGGG 6 2 0 11 10 0 0 +BoxI GACNNNNGTC 10 2 1 5 5 0 0 +BpiI GAAGAC 6 2 0 8 12 0 0 +BplI GAGNNNNNCTC 11 4 0 -9 -14 24 19 +BpmI CTGGAG 6 2 0 22 20 0 0 +Bpu10I CCTNAGC 7 2 0 2 5 0 0 +Bpu14I TTCGAA 6 2 0 2 4 0 0 +Bpu1102I GCTNAGC 7 2 0 2 5 0 0 +BpuAI GAAGAC 6 2 0 8 12 0 0 +BpuEI CTTGAG 6 2 0 22 20 0 0 +BpuMI CCSGG 5 2 0 2 3 0 0 +BpvUI CGATCG 6 2 0 4 2 0 0 +BsaI GGTCTC 6 2 0 7 11 0 0 +Bsa29I ATCGAT 6 2 0 2 4 0 0 +BsaAI YACGTR 6 2 1 3 3 0 0 +BsaBI GATNNNNATC 10 2 1 5 5 0 0 +BsaHI GRCGYC 6 2 0 2 4 0 0 +BsaJI CCNNGG 6 2 0 1 5 0 0 +BsaMI GAATGC 6 2 0 7 5 0 0 +BsaWI WCCGGW 6 2 0 1 5 0 0 +BsaXI ACNNNNNCTCC 11 4 0 -10 -13 21 18 +BsbI caacac 6 2 0 27 25 0 0 +Bsc4I CCNNNNNNNGG 11 2 0 7 4 0 0 +BscAI gcatc 5 2 0 9 11 0 0 +BscGI cccgt 5 0 0 0 0 0 0 +Bse1I ACTGG 5 2 0 6 4 0 0 +Bse8I GATNNNNATC 10 2 1 5 5 0 0 +Bse21I CCTNAGG 7 2 0 2 5 0 0 +Bse118I RCCGGY 6 2 0 1 5 0 0 +BseAI TCCGGA 6 2 0 1 5 0 0 +BseBI CCWGG 5 2 0 2 3 0 0 +BseCI ATCGAT 6 2 0 2 4 0 0 +BseDI CCNNGG 6 2 0 1 5 0 0 +Bse3DI GCAATG 6 2 0 8 6 0 0 +BseGI GGATG 5 2 0 7 5 0 0 +BseJI GATNNNNATC 10 2 1 5 5 0 0 +BseLI CCNNNNNNNGG 11 2 0 7 4 0 0 +BseMI GCAATG 6 2 0 8 6 0 0 +BseMII CTCAG 5 2 0 15 13 0 0 +BseNI ACTGG 5 2 0 6 4 0 0 +BsePI GCGCGC 6 2 0 1 5 0 0 +BseRI GAGGAG 6 2 0 16 14 0 0 +BseSI GKGCMC 6 2 0 5 1 0 0 +BseXI GCAGC 5 2 0 13 17 0 0 +BseX3I CGGCCG 6 2 0 1 5 0 0 +BseYI CCCAGC 6 2 0 1 5 0 0 +BsgI GTGCAG 6 2 0 22 20 0 0 +Bsh1236I CGCG 4 2 1 2 2 0 0 +Bsh1285I CGRYCG 6 2 0 4 2 0 0 +BshFI GGCC 4 2 1 2 2 0 0 +BshNI GGYRCC 6 2 0 1 5 0 0 +BshTI ACCGGT 6 2 0 1 5 0 0 +BshVI ATCGAT 6 2 0 2 4 0 0 +BsiI cacgag 6 2 0 1 5 0 0 +BsiEI CGRYCG 6 2 0 4 2 0 0 +BsiHKAI GWGCWC 6 2 0 5 1 0 0 +BsiHKCI CYCGRG 6 2 0 1 5 0 0 +BsiSI CCGG 4 2 0 1 3 0 0 +BsiWI CGTACG 6 2 0 1 5 0 0 +BsiYI ccnnnnnnngg 11 2 0 7 4 0 0 +BslI CCNNNNNNNGG 11 2 0 7 4 0 0 +BslFI GGGAC 5 2 0 15 19 0 0 +BsmI GAATGC 6 2 0 7 5 0 0 +BsmAI GTCTC 5 2 0 6 10 0 0 +BsmBI CGTCTC 6 2 0 7 11 0 0 +BsmFI GGGAC 5 2 0 15 19 0 0 +BsnI GGCC 4 2 1 2 2 0 0 +Bso31I GGTCTC 6 2 0 7 11 0 0 +BsoBI CYCGRG 6 2 0 1 5 0 0 +Bsp13I TCCGGA 6 2 0 1 5 0 0 +Bsp19I CCATGG 6 2 0 1 5 0 0 +Bsp24I gacnnnnnntgg 12 4 0 -9 -14 24 19 +Bsp68I TCGCGA 6 2 1 3 3 0 0 +Bsp119I TTCGAA 6 2 0 2 4 0 0 +Bsp120I GGGCCC 6 2 0 1 5 0 0 +Bsp143I GATC 4 2 0 -1 4 0 0 +Bsp1286I GDGCHC 6 2 0 5 1 0 0 +Bsp1407I TGTACA 6 2 0 1 5 0 0 +Bsp1720I GCTNAGC 7 2 0 2 5 0 0 +BspACI CCGC 4 2 0 1 3 0 0 +BspCNI CTCAG 5 2 0 14 12 0 0 +BspDI ATCGAT 6 2 0 2 4 0 0 +BspD6I gactc 5 2 0 9 11 0 0 +BspEI TCCGGA 6 2 0 1 5 0 0 +BspFNI CGCG 4 2 1 2 2 0 0 +BspGI ctggac 6 0 0 0 0 0 0 +BspHI TCATGA 6 2 0 1 5 0 0 +BspLI GGNNCC 6 2 1 3 3 0 0 +BspLU11I acatgt 6 2 0 1 5 0 0 +BspMI ACCTGC 6 2 0 10 14 0 0 +BspMII tccgga 6 2 0 1 5 0 0 +BspNCI ccaga 5 0 0 0 0 0 0 +BspOI GCTAGC 6 2 0 5 1 0 0 +BspPI GGATC 5 2 0 9 10 0 0 +BspQI GCTCTTC 7 2 0 8 11 0 0 +BspTI CTTAAG 6 2 0 1 5 0 0 +BspT104I TTCGAA 6 2 0 2 4 0 0 +BspT107I GGYRCC 6 2 0 1 5 0 0 +BspTNI GGTCTC 6 2 0 7 11 0 0 +BspXI ATCGAT 6 2 0 2 4 0 0 +BsrI ACTGG 5 2 0 6 4 0 0 +BsrBI CCGCTC 6 2 1 3 3 0 0 +BsrDI GCAATG 6 2 0 8 6 0 0 +BsrFI RCCGGY 6 2 0 1 5 0 0 +BsrGI TGTACA 6 2 0 1 5 0 0 +BsrSI ACTGG 5 2 0 6 4 0 0 +BssAI RCCGGY 6 2 0 1 5 0 0 +BssECI CCNNGG 6 2 0 1 5 0 0 +BssHII GCGCGC 6 2 0 1 5 0 0 +BssKI CCNGG 5 2 0 -1 5 0 0 +BssMI GATC 4 2 0 -1 4 0 0 +BssNI GRCGYC 6 2 0 2 4 0 0 +BssNAI GTATAC 6 2 1 3 3 0 0 +BssSI CACGAG 6 2 0 1 5 0 0 +BssT1I CCWWGG 6 2 0 1 5 0 0 +Bst6I CTCTTC 6 2 0 7 10 0 0 +Bst98I CTTAAG 6 2 0 1 5 0 0 +Bst1107I GTATAC 6 2 1 3 3 0 0 +BstACI GRCGYC 6 2 0 2 4 0 0 +BstAFI CTTAAG 6 2 0 1 5 0 0 +BstAPI GCANNNNNTGC 11 2 0 7 4 0 0 +BstAUI TGTACA 6 2 0 1 5 0 0 +BstBI TTCGAA 6 2 0 2 4 0 0 +Bst2BI CACGAG 6 2 0 1 5 0 0 +BstBAI YACGTR 6 2 1 3 3 0 0 +Bst4CI ACNGT 5 2 0 3 2 0 0 +BstC8I GCNNGC 6 2 1 3 3 0 0 +BstDEI CTNAG 5 2 0 1 4 0 0 +BstDSI CCRYGG 6 2 0 1 5 0 0 +BstEII GGTNACC 7 2 0 1 6 0 0 +BstENI CCTNNNNNAGG 11 2 0 5 6 0 0 +BstF5I GGATG 5 2 0 7 5 0 0 +BstFNI CGCG 4 2 1 2 2 0 0 +BstH2I RGCGCY 6 2 0 5 1 0 0 +BstHHI GCGC 4 2 0 3 1 0 0 +BstKTI GATC 4 2 0 3 1 0 0 +BstMAI GTCTC 5 2 0 6 10 0 0 +BstMBI GATC 4 2 0 -1 4 0 0 +BstMCI CGRYCG 6 2 0 4 2 0 0 +BstMWI GCNNNNNNNGC 11 2 0 7 4 0 0 +BstNI CCWGG 5 2 0 2 3 0 0 +BstNSI RCATGY 6 2 0 5 1 0 0 +BstOI CCWGG 5 2 0 2 3 0 0 +BstPI GGTNACC 7 2 0 1 6 0 0 +BstPAI GACNNNNGTC 10 2 1 5 5 0 0 +BstSCI CCNGG 5 2 0 -1 5 0 0 +BstSFI CTRYAG 6 2 0 1 5 0 0 +BstSLI GKGCMC 6 2 0 5 1 0 0 +BstSNI TACGTA 6 2 1 3 3 0 0 +BstUI CGCG 4 2 1 2 2 0 0 +Bst2UI CCWGG 5 2 0 2 3 0 0 +BstV1I GCAGC 5 2 0 13 17 0 0 +BstV2I GAAGAC 6 2 0 8 12 0 0 +BstXI CCANNNNNNTGG 12 2 0 8 4 0 0 +BstX2I RGATCY 6 2 0 1 5 0 0 +BstYI RGATCY 6 2 0 1 5 0 0 +BstZI CGGCCG 6 2 0 1 5 0 0 +BstZ17I GTATAC 6 2 1 3 3 0 0 +Bsu15I ATCGAT 6 2 0 2 4 0 0 +Bsu36I CCTNAGG 7 2 0 2 5 0 0 +BsuRI GGCC 4 2 1 2 2 0 0 +BsuTUI ATCGAT 6 2 0 2 4 0 0 +BtgI CCRYGG 6 2 0 1 5 0 0 +BtgZI GCGATG 6 2 0 16 20 0 0 +BthCI gcngc 5 2 0 4 1 0 0 +BtrI CACGTC 6 2 1 3 3 0 0 +BtsI GCAGTG 6 2 0 8 6 0 0 +BtsCI GGATG 5 2 0 7 5 0 0 +BtuMI TCGCGA 6 2 1 3 3 0 0 +BveI ACCTGC 6 2 0 10 14 0 0 +Cac8I GCNNGC 6 2 1 3 3 0 0 +CaiI CAGNNNCTG 9 2 0 6 3 0 0 +CauII ccsgg 5 2 0 2 3 0 0 +CciI TCATGA 6 2 0 1 5 0 0 +CciNI GCGGCCGC 8 2 0 2 6 0 0 +CdiI catcg 5 2 1 4 4 0 0 +CdpI gcggag 6 2 0 26 24 0 0 +CelII GCTNAGC 7 2 0 2 5 0 0 +CfoI GCGC 4 2 0 3 1 0 0 +CfrI YGGCCR 6 2 0 1 5 0 0 +Cfr9I CCCGGG 6 2 0 1 5 0 0 +Cfr10I RCCGGY 6 2 0 1 5 0 0 +Cfr13I GGNCC 5 2 0 1 4 0 0 +Cfr42I CCGCGG 6 2 0 4 2 0 0 +ChaI gatc 4 2 0 4 -1 0 0 +CjeI ccannnnnngt 11 4 0 -9 -15 26 20 +CjeNII gagnnnnngt 10 0 0 0 0 0 0 +CjePI ccannnnnnntc 12 4 0 -8 -14 26 20 +CjuI caynnnnnrtg 11 0 0 0 0 0 0 +CjuII caynnnnnctc 11 0 0 0 0 0 0 +ClaI ATCGAT 6 2 0 2 4 0 0 +CpoI CGGWCCG 7 2 0 2 5 0 0 +CseI GACGC 5 2 0 10 15 0 0 +CsiI ACCWGGT 7 2 0 1 6 0 0 +CspI CGGWCCG 7 2 0 2 5 0 0 +Csp6I GTAC 4 2 0 1 3 0 0 +Csp45I TTCGAA 6 2 0 2 4 0 0 +CspAI ACCGGT 6 2 0 1 5 0 0 +CspCI CAANNNNNGTGG 12 4 0 -12 -14 24 22 +CstMI aaggag 6 2 0 26 24 0 0 +CviAII CATG 4 2 0 1 3 0 0 +CviJI RGCY 4 2 1 2 2 0 0 +CviKI-1 RGCY 4 2 1 2 2 0 0 +CviQI GTAC 4 2 0 1 3 0 0 +CviRI tgca 4 2 1 2 2 0 0 +DdeI CTNAG 5 2 0 1 4 0 0 +DinI GGCGCC 6 2 1 3 3 0 0 +DpnI GATC 4 2 1 2 2 0 0 +DpnII GATC 4 2 0 -1 4 0 0 +DraI TTTAAA 6 2 1 3 3 0 0 +DraII RGGNCCY 7 2 0 2 5 0 0 +DraIII CACNNNGTG 9 2 0 6 3 0 0 +DraRI caagnac 7 2 0 27 25 0 0 +DrdI GACNNNNNNGTC 12 2 0 7 5 0 0 +DrdII gaacca 6 0 0 0 0 0 0 +DrdIV tacgac 6 2 0 26 24 0 0 +DriI GACNNNNNGTC 11 2 0 6 5 0 0 +DsaI ccrygg 6 2 0 1 5 0 0 +DseDI GACNNNNNNGTC 12 2 0 7 5 0 0 +EaeI YGGCCR 6 2 0 1 5 0 0 +EagI CGGCCG 6 2 0 1 5 0 0 +Eam1104I CTCTTC 6 2 0 7 10 0 0 +Eam1105I GACNNNNNGTC 11 2 0 6 5 0 0 +EarI CTCTTC 6 2 0 7 10 0 0 +EciI GGCGGA 6 2 0 17 15 0 0 +Ecl136II GAGCTC 6 2 1 3 3 0 0 +EclXI CGGCCG 6 2 0 1 5 0 0 +Eco24I GRGCYC 6 2 0 5 1 0 0 +Eco31I GGTCTC 6 2 0 7 11 0 0 +Eco32I GATATC 6 2 1 3 3 0 0 +Eco47I GGWCC 5 2 0 1 4 0 0 +Eco47III AGCGCT 6 2 1 3 3 0 0 +Eco52I CGGCCG 6 2 0 1 5 0 0 +Eco57I CTGAAG 6 2 0 22 20 0 0 +Eco72I CACGTG 6 2 1 3 3 0 0 +Eco81I CCTNAGG 7 2 0 2 5 0 0 +Eco88I CYCGRG 6 2 0 1 5 0 0 +Eco91I GGTNACC 7 2 0 1 6 0 0 +Eco105I TACGTA 6 2 1 3 3 0 0 +Eco130I CCWWGG 6 2 0 1 5 0 0 +Eco147I AGGCCT 6 2 1 3 3 0 0 +EcoHI ccsgg 5 2 0 -1 5 0 0 +EcoICRI GAGCTC 6 2 1 3 3 0 0 +Eco57MI CTGRAG 6 2 0 22 20 0 0 +EcoNI CCTNNNNNAGG 11 2 0 5 6 0 0 +EcoO65I GGTNACC 7 2 0 1 6 0 0 +EcoO109I RGGNCCY 7 2 0 2 5 0 0 +EcoRI GAATTC 6 2 0 1 5 0 0 +EcoRII CCWGG 5 2 0 -1 5 0 0 +EcoRV GATATC 6 2 1 3 3 0 0 +EcoT14I CCWWGG 6 2 0 1 5 0 0 +EcoT22I ATGCAT 6 2 0 5 1 0 0 +EcoT38I GRGCYC 6 2 0 5 1 0 0 +Eco53kI GAGCTC 6 2 1 3 3 0 0 +EgeI GGCGCC 6 2 1 3 3 0 0 +EheI GGCGCC 6 2 1 3 3 0 0 +ErhI CCWWGG 6 2 0 1 5 0 0 +EsaBC3I tcga 4 2 1 2 2 0 0 +EsaSSI gaccac 6 0 0 0 0 0 0 +EspI gctnagc 7 2 0 2 5 0 0 +Esp3I CGTCTC 6 2 0 7 11 0 0 +FaeI CATG 4 2 0 4 -1 0 0 +FaiI YATR 4 2 1 2 2 0 0 +FalI AAGNNNNNCTT 11 4 0 -9 -14 24 19 +FaqI GGGAC 5 2 0 15 19 0 0 +FatI CATG 4 2 0 -1 4 0 0 +FauI CCCGC 5 2 0 9 11 0 0 +FauNDI CATATG 6 2 0 2 4 0 0 +FbaI TGATCA 6 2 0 1 5 0 0 +FblI GTMKAC 6 2 0 2 4 0 0 +FinI gggac 5 0 0 0 0 0 0 +FmuI ggncc 5 2 0 4 1 0 0 +FnuDII cgcg 4 2 1 2 2 0 0 +Fnu4HI GCNGC 5 2 0 2 3 0 0 +FokI GGATG 5 2 0 14 18 0 0 +FriOI GRGCYC 6 2 0 5 1 0 0 +FseI GGCCGGCC 8 2 0 6 2 0 0 +FspI TGCGCA 6 2 1 3 3 0 0 +FspAI RTGCGCAY 8 2 1 4 4 0 0 +FspBI CTAG 4 2 0 1 3 0 0 +Fsp4HI GCNGC 5 2 0 2 3 0 0 +GdiII cggccr 6 2 0 1 5 0 0 +GlaI GCGC 4 2 1 2 2 0 0 +GluI GCNGC 5 2 0 2 3 0 0 +GsaI CCCAGC 6 2 0 5 1 0 0 +GsuI CTGGAG 6 2 0 22 20 0 0 +HaeI wggccw 6 2 1 3 3 0 0 +HaeII RGCGCY 6 2 0 5 1 0 0 +HaeIII GGCC 4 2 1 2 2 0 0 +HaeIV gaynnnnnrtc 11 4 0 -8 -14 25 20 +HapII CCGG 4 2 0 1 3 0 0 +HgaI GACGC 5 2 0 10 15 0 0 +HgiAI gwgcwc 6 2 0 5 1 0 0 +HgiCI ggyrcc 6 2 0 1 5 0 0 +HgiEII accnnnnnnggt 12 0 0 0 0 0 0 +HgiJII grgcyc 6 2 0 5 1 0 0 +HhaI GCGC 4 2 0 3 1 0 0 +Hin1I GRCGYC 6 2 0 2 4 0 0 +Hin1II CATG 4 2 0 4 -1 0 0 +Hin4I GAYNNNNNVTC 11 4 0 -9 -14 24 19 +Hin4II ccttc 5 2 0 11 10 0 0 +Hin6I GCGC 4 2 0 1 3 0 0 +HinP1I GCGC 4 2 0 1 3 0 0 +HincII GTYRAC 6 2 1 3 3 0 0 +HindII GTYRAC 6 2 1 3 3 0 0 +HindIII AAGCTT 6 2 0 1 5 0 0 +HinfI GANTC 5 2 0 1 4 0 0 +HpaI GTTAAC 6 2 1 3 3 0 0 +HpaII CCGG 4 2 0 1 3 0 0 +HphI GGTGA 5 2 0 13 12 0 0 +Hpy8I GTNNAC 6 2 1 3 3 0 0 +Hpy99I CGWCG 5 2 0 5 -1 0 0 +Hpy166II GTNNAC 6 2 1 3 3 0 0 +Hpy178III tcnnga 6 2 0 2 4 0 0 +Hpy188I TCNGA 5 2 0 3 2 0 0 +Hpy188III TCNNGA 6 2 0 2 4 0 0 +HpyAV CCTTC 5 2 0 11 10 0 0 +HpyCH4III ACNGT 5 2 0 3 2 0 0 +HpyCH4IV ACGT 4 2 0 1 3 0 0 +HpyCH4V TGCA 4 2 1 2 2 0 0 +HpyF3I CTNAG 5 2 0 1 4 0 0 +HpyF10VI GCNNNNNNNGC 11 2 0 7 4 0 0 +Hsp92I GRCGYC 6 2 0 2 4 0 0 +Hsp92II CATG 4 2 0 4 -1 0 0 +HspAI GCGC 4 2 0 1 3 0 0 +ItaI GCNGC 5 2 0 2 3 0 0 +KasI GGCGCC 6 2 0 1 5 0 0 +KflI GGGWCCC 7 2 0 2 5 0 0 +KpnI GGTACC 6 2 0 5 1 0 0 +Kpn2I TCCGGA 6 2 0 1 5 0 0 +KspI CCGCGG 6 2 0 4 2 0 0 +Ksp22I TGATCA 6 2 0 1 5 0 0 +Ksp632I ctcttc 6 2 0 7 10 0 0 +KspAI GTTAAC 6 2 1 3 3 0 0 +Kzo9I GATC 4 2 0 -1 4 0 0 +LguI GCTCTTC 7 2 0 8 11 0 0 +LpnI rgcgcy 6 2 1 3 3 0 0 +Lsp1109I GCAGC 5 2 0 13 17 0 0 +LweI GCATC 5 2 0 10 14 0 0 +MabI ACCWGGT 7 2 0 1 6 0 0 +MaeI CTAG 4 2 0 1 3 0 0 +MaeII ACGT 4 2 0 1 3 0 0 +MaeIII GTNAC 5 2 0 -1 5 0 0 +MalI GATC 4 2 1 2 2 0 0 +MaqI crttgac 7 2 0 28 26 0 0 +MauBI CGCGCGCG 8 2 0 2 6 0 0 +MbiI CCGCTC 6 2 1 3 3 0 0 +MboI GATC 4 2 0 -1 4 0 0 +MboII GAAGA 5 2 0 13 12 0 0 +McaTI gcgcgc 6 2 0 4 2 0 0 +McrI cgrycg 6 2 0 4 2 0 0 +MfeI CAATTG 6 2 0 1 5 0 0 +MflI RGATCY 6 2 0 1 5 0 0 +MhlI GDGCHC 6 2 0 5 1 0 0 +MjaIV gtnnac 6 0 0 0 0 0 0 +MlsI TGGCCA 6 2 1 3 3 0 0 +MluI ACGCGT 6 2 0 1 5 0 0 +MluNI TGGCCA 6 2 1 3 3 0 0 +MlyI GAGTC 5 2 1 10 10 0 0 +Mly113I GGCGCC 6 2 0 2 4 0 0 +MmeI TCCRAC 6 2 0 26 24 0 0 +MnlI CCTC 4 2 0 11 10 0 0 +Mph1103I ATGCAT 6 2 0 5 1 0 0 +MreI CGCCGGCG 8 2 0 2 6 0 0 +MroI TCCGGA 6 2 0 1 5 0 0 +MroNI GCCGGC 6 2 0 1 5 0 0 +MroXI GAANNNNTTC 10 2 1 5 5 0 0 +MscI TGGCCA 6 2 1 3 3 0 0 +MseI TTAA 4 2 0 1 3 0 0 +MslI CAYNNNNRTG 10 2 1 5 5 0 0 +MspI CCGG 4 2 0 1 3 0 0 +Msp20I TGGCCA 6 2 1 3 3 0 0 +MspA1I CMGCKG 6 2 1 3 3 0 0 +MspCI CTTAAG 6 2 0 1 5 0 0 +MspR9I CCNGG 5 2 0 2 3 0 0 +MssI GTTTAAAC 8 2 1 4 4 0 0 +MstI tgcgca 6 2 1 3 3 0 0 +MunI CAATTG 6 2 0 1 5 0 0 +MvaI CCWGG 5 2 0 2 3 0 0 +Mva1269I GAATGC 6 2 0 7 5 0 0 +MvnI CGCG 4 2 1 2 2 0 0 +MvrI CGATCG 6 2 0 4 2 0 0 +MwoI GCNNNNNNNGC 11 2 0 7 4 0 0 +NaeI GCCGGC 6 2 1 3 3 0 0 +NarI GGCGCC 6 2 0 2 4 0 0 +NciI CCSGG 5 2 0 2 3 0 0 +NcoI CCATGG 6 2 0 1 5 0 0 +NdeI CATATG 6 2 0 2 4 0 0 +NdeII GATC 4 2 0 -1 4 0 0 +NgoAVIII gacnnnnntga 11 4 0 -13 -15 24 22 +NgoMIV GCCGGC 6 2 0 1 5 0 0 +NhaXI caagrag 7 0 0 0 0 0 0 +NheI GCTAGC 6 2 0 1 5 0 0 +NlaIII CATG 4 2 0 4 -1 0 0 +NlaIV GGNNCC 6 2 1 3 3 0 0 +NlaCI catcac 6 2 0 25 23 0 0 +Nli3877I cycgrg 6 2 0 5 1 0 0 +NmeAIII GCCGAG 6 2 0 27 25 0 0 +NmeDI rccggy 6 4 0 -13 -8 13 18 +NmuCI GTSAC 5 2 0 -1 5 0 0 +NotI GCGGCCGC 8 2 0 2 6 0 0 +NruI TCGCGA 6 2 1 3 3 0 0 +NsbI TGCGCA 6 2 1 3 3 0 0 +NsiI ATGCAT 6 2 0 5 1 0 0 +NspI RCATGY 6 2 0 5 1 0 0 +NspV TTCGAA 6 2 0 2 4 0 0 +NspBII cmgckg 6 2 1 3 3 0 0 +OliI CACNNNNGTG 10 2 1 5 5 0 0 +PabI gtac 4 2 0 3 1 0 0 +PacI TTAATTAA 8 2 0 5 3 0 0 +PaeI GCATGC 6 2 0 5 1 0 0 +PaeR7I CTCGAG 6 2 0 1 5 0 0 +PagI TCATGA 6 2 0 1 5 0 0 +PalAI GGCGCGCC 8 2 0 2 6 0 0 +PasI CCCWGGG 7 2 0 2 5 0 0 +PauI GCGCGC 6 2 0 1 5 0 0 +PceI AGGCCT 6 2 1 3 3 0 0 +PciI ACATGT 6 2 0 1 5 0 0 +PciSI GCTCTTC 7 2 0 8 11 0 0 +PctI GAATGC 6 2 0 7 5 0 0 +PdiI GCCGGC 6 2 1 3 3 0 0 +PdmI GAANNNNTTC 10 2 1 5 5 0 0 +PfeI GAWTC 5 2 0 1 4 0 0 +Pfl23II CGTACG 6 2 0 1 5 0 0 +Pfl1108I tcgtag 6 0 0 0 0 0 0 +PflFI GACNNNGTC 9 2 0 4 5 0 0 +PflMI CCANNNNNTGG 11 2 0 7 4 0 0 +PfoI TCCNGGA 7 2 0 1 6 0 0 +PhoI GGCC 4 2 1 2 2 0 0 +PinAI ACCGGT 6 2 0 1 5 0 0 +PlaDI catcag 6 2 0 27 25 0 0 +PleI GAGTC 5 2 0 9 10 0 0 +Ple19I CGATCG 6 2 0 4 2 0 0 +PmaCI CACGTG 6 2 1 3 3 0 0 +PmeI GTTTAAAC 8 2 1 4 4 0 0 +PmlI CACGTG 6 2 1 3 3 0 0 +PpiI GAACNNNNNCTC 12 4 0 -8 -13 25 20 +PpsI GAGTC 5 2 0 9 10 0 0 +Ppu10I atgcat 6 2 0 1 5 0 0 +Ppu21I YACGTR 6 2 1 3 3 0 0 +PpuMI RGGWCCY 7 2 0 2 5 0 0 +PscI ACATGT 6 2 0 1 5 0 0 +PshAI GACNNNNGTC 10 2 1 5 5 0 0 +PshBI ATTAAT 6 2 0 2 4 0 0 +PsiI TTATAA 6 2 1 3 3 0 0 +Psp03I ggwcc 5 2 0 4 1 0 0 +Psp5II RGGWCCY 7 2 0 2 5 0 0 +Psp6I CCWGG 5 2 0 -1 5 0 0 +Psp1406I AACGTT 6 2 0 2 4 0 0 +Psp124BI GAGCTC 6 2 0 5 1 0 0 +PspCI CACGTG 6 2 1 3 3 0 0 +PspEI GGTNACC 7 2 0 1 6 0 0 +PspGI CCWGG 5 2 0 -1 5 0 0 +PspLI CGTACG 6 2 0 1 5 0 0 +PspN4I GGNNCC 6 2 1 3 3 0 0 +PspOMI GGGCCC 6 2 0 1 5 0 0 +PspOMII cgcccar 7 2 0 27 25 0 0 +PspPI GGNCC 5 2 0 1 4 0 0 +PspPPI RGGWCCY 7 2 0 2 5 0 0 +PspPRI ccycag 6 2 0 21 19 0 0 +PspXI VCTCGAGB 8 2 0 2 6 0 0 +PsrI GAACNNNNNNTAC 13 4 0 -8 -13 25 20 +PssI rggnccy 7 2 0 5 2 0 0 +PstI CTGCAG 6 2 0 5 1 0 0 +PsuI RGATCY 6 2 0 1 5 0 0 +PsyI GACNNNGTC 9 2 0 4 5 0 0 +PteI GCGCGC 6 2 0 1 5 0 0 +PvuI CGATCG 6 2 0 4 2 0 0 +PvuII CAGCTG 6 2 1 3 3 0 0 +RcaI TCATGA 6 2 0 1 5 0 0 +RceI catcgac 7 2 0 27 25 0 0 +RgaI GCGATCGC 8 2 0 5 3 0 0 +RigI GGCCGGCC 8 2 0 6 2 0 0 +RleAI cccaca 6 2 0 18 15 0 0 +RpaB5I cgrggac 7 2 0 27 25 0 0 +RruI TCGCGA 6 2 1 3 3 0 0 +RsaI GTAC 4 2 1 2 2 0 0 +RsaNI GTAC 4 2 0 1 3 0 0 +RseI CAYNNNNRTG 10 2 1 5 5 0 0 +RsrII CGGWCCG 7 2 0 2 5 0 0 +Rsr2I CGGWCCG 7 2 0 2 5 0 0 +SacI GAGCTC 6 2 0 5 1 0 0 +SacII CCGCGG 6 2 0 4 2 0 0 +SalI GTCGAC 6 2 0 1 5 0 0 +SanDI GGGWCCC 7 2 0 2 5 0 0 +SapI GCTCTTC 7 2 0 8 11 0 0 +SaqAI TTAA 4 2 0 1 3 0 0 +SatI GCNGC 5 2 0 2 3 0 0 +SauI cctnagg 7 2 0 2 5 0 0 +Sau96I GGNCC 5 2 0 1 4 0 0 +Sau3AI GATC 4 2 0 -1 4 0 0 +SbfI CCTGCAGG 8 2 0 6 2 0 0 +ScaI AGTACT 6 2 1 3 3 0 0 +SchI GAGTC 5 2 1 10 10 0 0 +SciI ctcgag 6 2 1 3 3 0 0 +ScrFI CCNGG 5 2 0 2 3 0 0 +SdaI CCTGCAGG 8 2 0 6 2 0 0 +SdeAI cagrag 6 2 0 27 25 0 0 +SdeOSI gacnnnnrtga 11 4 0 -12 -14 23 21 +SduI GDGCHC 6 2 0 5 1 0 0 +SecI ccnngg 6 2 0 1 5 0 0 +SelI cgcg 4 2 0 -1 4 0 0 +SetI ASST 4 2 0 4 -1 0 0 +SexAI ACCWGGT 7 2 0 1 6 0 0 +SfaAI GCGATCGC 8 2 0 5 3 0 0 +SfaNI GCATC 5 2 0 10 14 0 0 +SfcI CTRYAG 6 2 0 1 5 0 0 +SfeI ctryag 6 2 0 1 5 0 0 +SfiI GGCCNNNNNGGCC 13 2 0 8 5 0 0 +SfoI GGCGCC 6 2 1 3 3 0 0 +Sfr274I CTCGAG 6 2 0 1 5 0 0 +Sfr303I CCGCGG 6 2 0 4 2 0 0 +SfuI TTCGAA 6 2 0 2 4 0 0 +SgfI GCGATCGC 8 2 0 5 3 0 0 +SgrAI CRCCGGYG 8 2 0 2 6 0 0 +SgrBI CCGCGG 6 2 0 4 2 0 0 +SgrDI CGTCGACG 8 2 0 2 6 0 0 +SgsI GGCGCGCC 8 2 0 2 6 0 0 +SimI gggtc 5 2 0 2 5 0 0 +SinI GGWCC 5 2 0 1 4 0 0 +SlaI CTCGAG 6 2 0 1 5 0 0 +SmaI CCCGGG 6 2 1 3 3 0 0 +SmiI ATTTAAAT 8 2 1 4 4 0 0 +SmiMI CAYNNNNRTG 10 2 1 5 5 0 0 +SmlI CTYRAG 6 2 0 1 5 0 0 +SmoI CTYRAG 6 2 0 1 5 0 0 +SmuI CCCGC 5 2 0 9 11 0 0 +SnaI gtatac 6 0 0 0 0 0 0 +SnaBI TACGTA 6 2 1 3 3 0 0 +SpeI ACTAGT 6 2 0 1 5 0 0 +SphI GCATGC 6 2 0 5 1 0 0 +SplI cgtacg 6 2 0 1 5 0 0 +SpoDI gcggrag 7 0 0 0 0 0 0 +SrfI GCCCGGGC 8 2 1 4 4 0 0 +Sse9I AATT 4 2 0 -1 4 0 0 +Sse232I cgccggcg 8 2 0 2 6 0 0 +Sse8387I CCTGCAGG 8 2 0 6 2 0 0 +Sse8647I aggwcct 7 2 0 2 5 0 0 +SseBI AGGCCT 6 2 1 3 3 0 0 +SsiI CCGC 4 2 0 1 3 0 0 +SspI AATATT 6 2 1 3 3 0 0 +SspDI GGCGCC 6 2 0 1 5 0 0 +SspD5I ggtga 5 2 1 13 13 0 0 +SstI GAGCTC 6 2 0 5 1 0 0 +SstII CCGCGG 6 2 0 4 2 0 0 +Sth132I cccg 4 2 0 8 12 0 0 +Sth302II ccgg 4 2 1 2 2 0 0 +StrI CTCGAG 6 2 0 1 5 0 0 +StsI ggatg 5 2 0 15 19 0 0 +StuI AGGCCT 6 2 1 3 3 0 0 +StyI CCWWGG 6 2 0 1 5 0 0 +StyD4I CCNGG 5 2 0 -1 5 0 0 +SwaI ATTTAAAT 8 2 1 4 4 0 0 +TaaI ACNGT 5 2 0 3 2 0 0 +TaiI ACGT 4 2 0 4 -1 0 0 +TaqI TCGA 4 2 0 1 3 0 0 +TaqII GACCGA 6 2 0 17 15 0 0 +TaqII CACCCA 6 2 0 17 15 0 0 +TasI AATT 4 2 0 -1 4 0 0 +TatI WGTACW 6 2 0 1 5 0 0 +TauI GCSGC 5 2 0 4 1 0 0 +TfiI GAWTC 5 2 0 1 4 0 0 +TliI CTCGAG 6 2 0 1 5 0 0 +Tru1I TTAA 4 2 0 1 3 0 0 +Tru9I TTAA 4 2 0 1 3 0 0 +TscAI CASTG 5 2 0 7 -3 0 0 +TseI GCWGC 5 2 0 1 4 0 0 +TsoI TARCCA 6 2 0 17 15 0 0 +Tsp45I GTSAC 5 2 0 -1 5 0 0 +Tsp509I AATT 4 2 0 -1 4 0 0 +Tsp4CI acngt 5 2 0 3 2 0 0 +TspDTI ATGAA 5 2 0 16 14 0 0 +TspEI AATT 4 2 0 -1 4 0 0 +TspGWI ACGGA 5 2 0 16 14 0 0 +TspMI CCCGGG 6 2 0 1 5 0 0 +TspRI CASTG 5 2 0 7 -3 0 0 +TssI gagnnnctc 9 0 0 0 0 0 0 +TstI CACNNNNNNTCC 12 4 0 -9 -14 24 19 +TsuI gcgac 5 0 0 0 0 0 0 +Tth111I GACNNNGTC 9 2 0 4 5 0 0 +Tth111II caarca 6 2 0 17 15 0 0 +UbaF9I tacnnnnnrtgt 12 0 0 0 0 0 0 +UbaF11I tcgta 5 0 0 0 0 0 0 +UbaF12I ctacnnngtc 10 0 0 0 0 0 0 +UbaF13I gagnnnnnnctgg 13 0 0 0 0 0 0 +UbaF14I ccannnnntcg 11 0 0 0 0 0 0 +UbaPI cgaacg 6 0 0 0 0 0 0 +UnbI ggncc 5 2 0 -1 5 0 0 +Van91I CCANNNNNTGG 11 2 0 7 4 0 0 +Vha464I CTTAAG 6 2 0 1 5 0 0 +VneI GTGCAC 6 2 0 1 5 0 0 +VpaK11AI ggwcc 5 2 0 -1 5 0 0 +VpaK11BI GGWCC 5 2 0 1 4 0 0 +VspI ATTAAT 6 2 0 2 4 0 0 +XagI CCTNNNNNAGG 11 2 0 5 6 0 0 +XapI RAATTY 6 2 0 1 5 0 0 +XbaI TCTAGA 6 2 0 1 5 0 0 +XceI RCATGY 6 2 0 5 1 0 0 +XcmI CCANNNNNNNNNTGG 15 2 0 8 7 0 0 +XhoI CTCGAG 6 2 0 1 5 0 0 +XhoII RGATCY 6 2 0 1 5 0 0 +XmaI CCCGGG 6 2 0 1 5 0 0 +XmaIII cggccg 6 2 0 1 5 0 0 +XmaCI CCCGGG 6 2 0 1 5 0 0 +XmaJI CCTAGG 6 2 0 1 5 0 0 +XmiI GTMKAC 6 2 0 2 4 0 0 +XmnI GAANNNNTTC 10 2 1 5 5 0 0 +XspI CTAG 4 2 0 1 3 0 0 +ZraI GACGTC 6 2 1 3 3 0 0 +ZrmI AGTACT 6 2 1 3 3 0 0 +Zsp2I ATGCAT 6 2 0 5 1 0 0