From: martinahansen <martinahansen@74ccb610-7750-0410-82ae-013aeee3265d>
Date: Mon, 13 Dec 2010 14:00:55 +0000 (+0000)
Subject: added QA_Solexa_report.sh
X-Git-Url: https://git.donarmstrong.com/?a=commitdiff_plain;h=15eff44d72eb5d662033a5c1de3b72b02cda1e55;hp=3de30d5afa0d1a33ea84c0db5849e979967902de;p=biopieces.git

added QA_Solexa_report.sh

git-svn-id: http://biopieces.googlecode.com/svn/trunk@1187 74ccb610-7750-0410-82ae-013aeee3265d
---

diff --git a/bp_scripts/QA_Solexa_report.sh b/bp_scripts/QA_Solexa_report.sh
new file mode 100755
index 0000000..43f1ff1
--- /dev/null
+++ b/bp_scripts/QA_Solexa_report.sh
@@ -0,0 +1,167 @@
+#!/bin/bash
+
+# Copyright (C) 2010 Martin A. Hansen (mail@maasha.dk).
+
+# This program is free software; you can redistribute it and/or
+# modify it under the terms of the GNU General Public License
+# as published by the Free Software Foundation; either version 2
+# of the License, or (at your option) any later version.
+
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+# GNU General Public License for more details.
+
+# You should have received a copy of the GNU General Public License
+# along with this program; if not, write to the Free Software
+# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
+
+# http://www.gnu.org/copyleft/gpl.html
+
+fastq_files=$@
+
+if [ ! $1 ]; then
+    echo
+    echo "QA_Solexa_report.sh generates a quality assurance report for"
+    echo "each of a given number of Solexa/Illumina FASTQ files."
+    echo
+    echo "Usage: `basename $0` <FASTQ file(s)>"
+    echo
+    exit
+fi
+
+function puts
+{
+    msg=$1
+
+    echo $msg >> $out_file
+}
+
+function pcat
+{
+    file=$1
+
+    cat $file >> $out_file
+}
+
+for fastq_file in $fastq_files; do
+    base=`basename $fastq_file`
+    date=`date | sed 's/[ :]/_/g'`
+    tmp_dir="$HOME/QA_Solexa_report_$date"
+    out_file="$tmp_dir/QA_Solexa_report_$date.txt"
+    plot_scores="$tmp_dir/plot_scores.txt"
+    plot_lendist="$tmp_dir/plot_lendist.txt"
+    freq_table="$tmp_dir/freq_table.txt"
+    read_count="$tmp_dir/read_count.txt"
+    read_length="$tmp_dir/read_length.txt"
+    top30_reads="$tmp_dir/top30_reads.txt"
+
+    if [ ! -d $tmp_dir ]; then
+        mkdir $tmp_dir
+    fi
+
+    read_fastq -n 1 -i $fastq_file |
+    write_tab -k SEQ_LEN -o $read_length -x
+
+    echo "" && echo "Plotting scores and length distributions ... "
+    read_fastq -i $fastq_file |
+    progress_meter -c 10000 |
+    count_records -o $read_count |
+    plot_scores -o $plot_scores -Y "Mean score" -X "Sequence length" |
+    trim_seq |
+    bin_vals -k SEQ_LEN |
+    plot_lendist -k SEQ_LEN_BIN -o $plot_lendist -Y "Count" -X "Sequence Length (5nt bins)" -x
+
+    echo "" && echo "Running composition analysis on sequences ... "
+    read_fastq -i $fastq_file |
+    progress_meter -c 10000 |
+    create_weight_matrix -p |
+    flip_tab |
+    write_tab -o $freq_table -x
+
+    echo "" && echo "Locating top 30 unique reads ... "
+    read_fastq -i $fastq_file |
+    progress_meter -c 10000 |
+    uniq_seq -c |
+    sort_records -rk SEQ_COUNTn |
+    head_records -n 30 |
+    write_tab -ck SEQ_COUNT,SEQ -o $top30_reads -x
+
+    echo "" && echo -n "Generating report ... "
+    puts ""
+    puts ""
+    puts ""
+    puts "QA Solexa Report"
+    puts "============="
+    puts ""
+    puts ""
+    puts ""
+    puts "Date: `date`"
+    puts ""
+    puts "File: `pwd`/$fastq_file"
+    puts ""
+    puts ""
+    puts "Sequence analysis"
+    puts "-----------------"
+    puts ""
+    puts ""
+    puts "Read length:"
+    puts ""
+    pcat $read_length
+    puts ""
+    puts "Read count:"
+    puts ""
+    pcat $read_count
+    puts ""
+    puts ""
+    puts "Quality score means"
+    puts "-------------------"
+    puts ""
+    puts ""
+    puts "The mean scores of the untrimmed sequences:"
+    puts ""
+    pcat $plot_scores
+    puts ""
+    puts ""
+    puts "Sequence length distribution"
+    puts "----------------------------"
+    puts ""
+    puts ""
+    puts "The length distribution of trimmed reads where the lengths are binned in buckets of size 5:"
+    puts ""
+    pcat $plot_lendist
+    puts ""
+    puts ""
+    puts "Residue frequency analysis"
+    puts "--------------------------"
+    puts ""
+    puts ""
+    puts "The below table contains the residue frequency (in percent) of all base positions:"
+    puts ""
+    pcat $freq_table
+    puts ""
+    puts ""
+    puts "Top 30 reads"
+    puts "--------------------------"
+    puts ""
+    puts ""
+    pcat $top30_reads
+    puts ""
+    puts ""
+    puts "end."
+
+    rm $plot_scores
+    rm $plot_lendist
+    rm $freq_table
+    rm $read_count
+    rm $read_length
+    rm $top30_reads
+
+    echo "done."
+
+    echo ""
+    echo "Report located here: $out_file"
+    echo ""
+done
+
+echo "All done."