From: martinahansen <martinahansen@74ccb610-7750-0410-82ae-013aeee3265d>
Date: Mon, 21 Jul 2008 05:17:55 +0000 (+0000)
Subject: added soap_seq
X-Git-Url: https://git.donarmstrong.com/?a=commitdiff_plain;h=09ac3b416c60618e4ceb372d77ba51a798a0ad41;p=biopieces.git

added soap_seq

git-svn-id: http://biopieces.googlecode.com/svn/trunk@180 74ccb610-7750-0410-82ae-013aeee3265d
---

diff --git a/bp_bin/soap_seq b/bp_bin/soap_seq
new file mode 100755
index 0000000..4cd1d44
--- /dev/null
+++ b/bp_bin/soap_seq
@@ -0,0 +1,6 @@
+#!/usr/bin/env perl
+
+use warnings;
+use strict;
+
+use Maasha::Biopieces;
diff --git a/code_perl/Maasha/Biopieces.pm b/code_perl/Maasha/Biopieces.pm
index e883884..ce93a0e 100644
--- a/code_perl/Maasha/Biopieces.pm
+++ b/code_perl/Maasha/Biopieces.pm
@@ -216,6 +216,7 @@ sub run_script
     elsif ( $script eq "create_blast_db" )          { script_create_blast_db(           $in, $out, $options ) }
     elsif ( $script eq "blast_seq" )                { script_blast_seq(                 $in, $out, $options ) }
     elsif ( $script eq "blat_seq" )                 { script_blat_seq(                  $in, $out, $options ) }
+    elsif ( $script eq "soap_seq" )                 { script_soap_seq(                  $in, $out, $options ) }
     elsif ( $script eq "match_seq" )                { script_match_seq(                 $in, $out, $options ) }
     elsif ( $script eq "create_vmatch_index" )      { script_create_vmatch_index(       $in, $out, $options ) }
     elsif ( $script eq "vmatch_seq" )               { script_vmatch_seq(                $in, $out, $options ) }
@@ -569,6 +570,14 @@ sub get_options
             ooc|c
         );
     }
+    elsif ( $script eq "soap_seq" )
+    {
+        @options = qw(
+            in_file|i=s
+            genome|g=s
+            cpus|c=s
+        );
+    }
     elsif ( $script eq "match_seq" )
     {
         @options = qw(
@@ -1007,7 +1016,9 @@ sub get_options
     Maasha::Common::error( qq(no --database or --genome specified) )    if $script eq "blast_seq" and not $options{ "genome" } and not $options{ "database" };
     Maasha::Common::error( qq(both --database and --genome specified) ) if $script eq "blast_seq" and $options{ "genome" } and $options{ "database" };
     Maasha::Common::error( qq(no --index_name or --genome specified) )  if $script eq "vmatch_seq" and not $options{ "genome" } and not $options{ "index_name" };
-    Maasha::Common::error( qq(both --index and --genome specified) )    if $script eq "vmatch_seq" and $options{ "genome" } and $options{ "index" };
+    Maasha::Common::error( qq(both --index and --genome specified) )    if $script eq "vmatch_seq" and $options{ "genome" } and $options{ "index_name" };
+    Maasha::Common::error( qq(no --in_file or --genome specified) )     if $script eq "soap_seq" and not $options{ "genome" } and not $options{ "in_file" };
+    Maasha::Common::error( qq(both --in_file and --genome specified) )  if $script eq "soap_seq" and $options{ "genome" } and $options{ "in_file" };
     Maasha::Common::error( qq(no --genome specified) )                  if $script =~ /format_genome|get_genome_seq|get_genome_align|get_genome_phastcons|blat_seq|plot_phastcons_profiles|plot_karyogram/ and not $options{ "genome" };
     Maasha::Common::error( qq(no --key specified) )                     if $script =~ /plot_lendist|plot_histogram/ and not $options{ "key" };
     Maasha::Common::error( qq(no --keys speficied) )                    if $script =~ /sort_records|count_vals|sum_vals|mean_vals|median_vals|length_vals/ and not $options{ "keys" };
@@ -3404,7 +3415,7 @@ sub script_blast_seq
     $options->{ "filter" } = "T" if $options->{ "filter" };
     $options->{ "cpus" } ||= 1;
 
-    $options->{ "database" } = "$ENV{ 'BP_DATA' }/genomes/$options->{ 'genome' }/blast/$options->{ 'genome' }.fna"if $options->{ 'genome' };
+    $options->{ "database" } = "$ENV{ 'BP_DATA' }/genomes/$options->{ 'genome' }/blast/$options->{ 'genome' }.fna" if $options->{ 'genome' };
 
     $tmp_in  = "$BP_TMP/blast_query.seq";
     $tmp_out = "$BP_TMP/blast.result";
@@ -3558,6 +3569,94 @@ sub script_blat_seq
 }
 
 
+sub script_soap_seq
+{
+    # Martin A. Hansen, July 2008.
+
+    # soap sequences in stream against a given file or genome.
+
+    my ( $in,        # handle to in stream
+         $out,       # handle to out stream
+         $options,   # options hash
+       ) = @_;
+
+    # Returns nothing.
+
+    my ( $genome, $tmp_in, $tmp_out, $fh_in, $fh_out, $record, $line, @fields );
+
+    $options->{ "cpus" } ||= 1;
+
+    $options->{ "in_file" } = "$ENV{ 'BP_DATA' }/genomes/$options->{ 'genome' }/fasta/$options->{ 'genome' }.fna" if $options->{ 'genome' };
+
+    $tmp_in  = "$BP_TMP/soap_query.seq";
+    $tmp_out = "$BP_TMP/soap.result";
+
+    $fh_out = Maasha::Common::write_open( $tmp_in );
+
+    while ( $record = get_record( $in ) ) 
+    {
+        if ( $record->{ "SEQ_NAME" } and $record->{ "SEQ" } ) {
+            Maasha::Fasta::put_entry( [ $record->{ "SEQ_NAME" }, $record->{ "SEQ" } ], $fh_out );
+        }
+
+        put_record( $record, $out );
+    }
+
+    close $fh_out;
+
+    Maasha::Common::run( "soap", "-r 2 -a $tmp_in -p $options->{ 'cpus' } -d $options->{ 'in_file' } -o $tmp_out > /dev/null 2>&1", 1 );
+
+    unlink $tmp_in;
+
+    $fh_out = Maasha::Common::read_open( $tmp_out );
+
+    undef $record;
+
+    while ( $line = <$fh_out> )
+    {
+        chomp $line;
+
+
+print Dumper( $line );
+
+        next if $line =~ /^#/;
+
+        @fields = split /\s+/, $line;
+
+        $record->{ "REC_TYPE" }   = "BLAST";
+        $record->{ "Q_ID" }       = $fields[ 0 ];
+        $record->{ "S_ID" }       = $fields[ 1 ];
+        $record->{ "IDENT" }      = $fields[ 2 ];
+        $record->{ "ALIGN_LEN" }  = $fields[ 3 ];
+        $record->{ "MISMATCHES" } = $fields[ 4 ];
+        $record->{ "GAPS" }       = $fields[ 5 ];
+        $record->{ "Q_BEG" }      = $fields[ 6 ] - 1; # BLAST is 1-based
+        $record->{ "Q_END" }      = $fields[ 7 ] - 1; # BLAST is 1-based
+        $record->{ "S_BEG" }      = $fields[ 8 ] - 1; # BLAST is 1-based
+        $record->{ "S_END" }      = $fields[ 9 ] - 1; # BLAST is 1-based
+        $record->{ "E_VAL" }      = $fields[ 10 ];
+        $record->{ "BIT_SCORE" }  = $fields[ 11 ];
+
+        if ( $record->{ "S_BEG" } > $record->{ "S_END" } )
+        {
+            $record->{ "STRAND" } = '-';
+
+            ( $record->{ "S_BEG" }, $record->{ "S_END" } ) = ( $record->{ "S_END" }, $record->{ "S_BEG" } );
+        }
+        else
+        {
+            $record->{ "STRAND" } = '+';
+        }
+
+        put_record( $record, $out );
+    }
+
+    close $fh_out;
+
+    unlink $tmp_out;
+}
+
+
 sub script_match_seq
 {
     # Martin A. Hansen, August 2007.