X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=sequence.hpp;h=94d7d29b6117da80862e9fc8b3d0855c4adb8edd;hb=4de093e3b92bdab1579565cb2873553412f6671e;hp=e094fb520f7ad26c96cbcfa7226e194bd883f690;hpb=bfbc55964f1977da72c2cea984288a427d370a59;p=mothur.git

diff --git a/sequence.hpp b/sequence.hpp
index e094fb5..94d7d29 100644
--- a/sequence.hpp
+++ b/sequence.hpp
@@ -8,32 +8,72 @@
  *  Created by Pat Schloss on 12/15/08.
  *  Copyright 2008 Patrick D. Schloss. All rights reserved.
  *
+ *	A sequence object has three components: i) an accession number / name, ii) the unaligned primary sequence, iii) a
+ *	pairwise aligned sequence, and iv) a sequence that is aligned to a reference alignment.  This class has methods
+ *	to set and get these values for the other classes where they are needed.  *
+ *
  */
-using namespace std;
 
 #include "mothur.h"
-
+#include "mothurout.h"
 
 /**************************************************************************************************/
 
 class Sequence {
 public:
 	Sequence();
+	Sequence(string, string);
 	Sequence(ifstream&);
+	Sequence(istringstream&);
+	
+	//these constructors just set the unaligned string to save space
+	Sequence(string, string, string);  
+	Sequence(ifstream&, string);
+	Sequence(istringstream&, string);
+	
 	void setName(string);
 	void setUnaligned(string);
 	void setPairwise(string);
 	void setAligned(string);
+	void setLength();
+	void reverseComplement();
+	void trim(int);
+	
 	string convert2ints();
-	string getSeqName();
+	string getName();
 	string getAligned();
 	string getPairwise();
 	string getUnaligned();
+	string getInlineSeq();
+	int getNumBases();
+	int getStartPos();
+	int getEndPos();
+	void padToPos(int);
+	void padFromPos(int);
+	int getAlignLength();
+	int getAmbigBases();
+	void removeAmbigBases();
+	int getLongHomoPolymer();
+	bool getIsAligned();
+	void printSequence(ostream&);
+	
 private:
+	MothurOut* m;
+	void initialize();
+	string getSequenceString(ifstream&);
+	string getCommentString(ifstream&);
+	string getSequenceString(istringstream&);
+	string getCommentString(istringstream&);
 	string name;
 	string unaligned;
-	string pairwise;
 	string aligned;
+	string pairwise;
+	int numBases;
+	int alignmentLength;
+	bool isAligned;
+	int longHomoPolymer;
+	int ambigBases;
+	int startPos, endPos;
 };
 
 /**************************************************************************************************/