X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=sequence.cpp;h=4c631a23451d4d9723aec3a29bed423737b243ba;hb=75cff1a83a64cd46e9dd603e02597a2bb6a76119;hp=96662bc36d4b77e1cb8b7a884f4e8460969033f4;hpb=5c5c0428f6d548c28a8b903ac80efed4f92d59db;p=mothur.git diff --git a/sequence.cpp b/sequence.cpp index 96662bc..4c631a2 100644 --- a/sequence.cpp +++ b/sequence.cpp @@ -20,6 +20,10 @@ Sequence::Sequence(string newName, string sequence) { m = MothurOut::getInstance(); initialize(); name = newName; + + for (int i = 0; i < name.length(); i++) { + if (name[i] == ':') { name[i] = '_'; m->changedSeqNames = true; } + } //setUnaligned removes any gap characters for us setUnaligned(sequence); @@ -36,6 +40,10 @@ Sequence::Sequence(string newName, string sequence, string justUnAligned) { m = MothurOut::getInstance(); initialize(); name = newName; + + for (int i = 0; i < name.length(); i++) { + if (name[i] == ':') { name[i] = '_'; m->changedSeqNames = true; } + } //setUnaligned removes any gap characters for us setUnaligned(sequence); @@ -53,11 +61,9 @@ Sequence::Sequence(istringstream& fastaString){ m = MothurOut::getInstance(); initialize(); - fastaString >> name; - - if (name.length() != 0) { + name = getSequenceName(fastaString); - name = name.substr(1); + if (!m->control_pressed) { string sequence; //read comments @@ -84,8 +90,7 @@ Sequence::Sequence(istringstream& fastaString){ setUnaligned(sequence); if ((numAmbig / (float) numBases) > 0.25) { m->mothurOut("[WARNING]: We found more than 25% of the bases in sequence " + name + " to be ambiguous. Mothur is not setup to process protein sequences."); m->mothurOutEndLine(); } - - }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaString.tellg()) + ". Blank name."); m->mothurOutEndLine(); } + } } catch(exception& e) { @@ -100,11 +105,9 @@ Sequence::Sequence(istringstream& fastaString, string JustUnaligned){ m = MothurOut::getInstance(); initialize(); - fastaString >> name; - - if (name.length() != 0) { + name = getSequenceName(fastaString); - name = name.substr(1); + if (!m->control_pressed) { string sequence; //read comments @@ -131,7 +134,7 @@ Sequence::Sequence(istringstream& fastaString, string JustUnaligned){ if ((numAmbig / (float) numBases) > 0.25) { m->mothurOut("[WARNING]: We found more than 25% of the bases in sequence " + name + " to be ambiguous. Mothur is not setup to process protein sequences."); m->mothurOutEndLine(); } - }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaString.tellg()) + ". Blank name."); m->mothurOutEndLine(); } + } } catch(exception& e) { @@ -147,11 +150,9 @@ Sequence::Sequence(ifstream& fastaFile){ try { m = MothurOut::getInstance(); initialize(); - fastaFile >> name; - - if (name.length() != 0) { + name = getSequenceName(fastaFile); - name = name.substr(1); + if (!m->control_pressed) { string sequence; @@ -181,7 +182,7 @@ Sequence::Sequence(ifstream& fastaFile){ if ((numAmbig / (float) numBases) > 0.25) { m->mothurOut("[WARNING]: We found more than 25% of the bases in sequence " + name + " to be ambiguous. Mothur is not setup to process protein sequences."); m->mothurOutEndLine(); } - }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaFile.tellg()) + ". Blank name."); m->mothurOutEndLine(); } + } } catch(exception& e) { @@ -195,13 +196,11 @@ Sequence::Sequence(ifstream& fastaFile, string& extraInfo, bool getInfo){ try { m = MothurOut::getInstance(); initialize(); - fastaFile >> name; extraInfo = ""; - if (name.length() != 0) { - - name = name.substr(1); - + name = getSequenceName(fastaFile); + + if (!m->control_pressed) { string sequence; //read comments @@ -233,8 +232,7 @@ Sequence::Sequence(ifstream& fastaFile, string& extraInfo, bool getInfo){ setUnaligned(sequence); if ((numAmbig / (float) numBases) > 0.25) { m->mothurOut("[WARNING]: We found more than 25% of the bases in sequence " + name + " to be ambiguous. Mothur is not setup to process protein sequences."); m->mothurOutEndLine(); } - - }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaFile.tellg()) + ". Blank name."); m->mothurOutEndLine(); } + } } catch(exception& e) { @@ -248,10 +246,9 @@ Sequence::Sequence(ifstream& fastaFile, string JustUnaligned){ try { m = MothurOut::getInstance(); initialize(); - fastaFile >> name; + name = getSequenceName(fastaFile); - if (name.length() != 0) { - name = name.substr(1); + if (!m->control_pressed) { string sequence; //read comments @@ -279,7 +276,7 @@ Sequence::Sequence(ifstream& fastaFile, string JustUnaligned){ if ((numAmbig / (float) numBases) > 0.25) { m->mothurOut("[WARNING]: We found more than 25% of the bases in sequence " + name + " to be ambiguous. Mothur is not setup to process protein sequences."); m->mothurOutEndLine(); } - }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaFile.tellg()) + ". Blank name."); m->mothurOutEndLine(); } + } } catch(exception& e) { @@ -287,7 +284,54 @@ Sequence::Sequence(ifstream& fastaFile, string JustUnaligned){ exit(1); } } - +//******************************************************************************************************************** +string Sequence::getSequenceName(ifstream& fastaFile) { + try { + string name = ""; + + fastaFile >> name; + + if (name.length() != 0) { + + name = name.substr(1); + + for (int i = 0; i < name.length(); i++) { + if (name[i] == ':') { name[i] = '_'; m->changedSeqNames = true; } + } + + }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaFile.tellg()) + ". Blank name."); m->mothurOutEndLine(); m->control_pressed = true; } + + return name; + } + catch(exception& e) { + m->errorOut(e, "Sequence", "getSequenceName"); + exit(1); + } +} +//******************************************************************************************************************** +string Sequence::getSequenceName(istringstream& fastaFile) { + try { + string name = ""; + + fastaFile >> name; + + if (name.length() != 0) { + + name = name.substr(1); + + for (int i = 0; i < name.length(); i++) { + if (name[i] == ':') { name[i] = '_'; m->changedSeqNames = true; } + } + + }else{ m->mothurOut("Error in reading your fastafile, at position " + toString(fastaFile.tellg()) + ". Blank name."); m->mothurOutEndLine(); m->control_pressed = true; } + + return name; + } + catch(exception& e) { + m->errorOut(e, "Sequence", "getSequenceName"); + exit(1); + } +} //******************************************************************************************************************** string Sequence::getSequenceString(ifstream& fastaFile, int& numAmbig) { try { @@ -531,6 +575,15 @@ string Sequence::getUnaligned(){ int Sequence::getNumBases(){ return numBases; } +//******************************************************************************************************************** + +int Sequence::getNumNs(){ + int numNs = 0; + for (int i = 0; i < unaligned.length(); i++) { + if(toupper(unaligned[i]) == 'N') { numNs++; } + } + return numNs; +} //******************************************************************************************************************** @@ -719,6 +772,8 @@ void Sequence::trim(int length){ if(numBases > length){ unaligned = unaligned.substr(0,length); numBases = length; + aligned = ""; + isAligned = 0; } }