X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=seqsummarycommand.cpp;h=7226f9ddac16e61ebc2239ff4d868474b990ec25;hb=f07bf12e0d04340698aff7a36d2fee7c959ffe59;hp=82a1d2473a0bc35282abd46a5998ae65fbcce1bf;hpb=510b1cfc25cd79391d6973ca20c5ec25fb1bb3b2;p=mothur.git diff --git a/seqsummarycommand.cpp b/seqsummarycommand.cpp index 82a1d24..7226f9d 100644 --- a/seqsummarycommand.cpp +++ b/seqsummarycommand.cpp @@ -12,7 +12,7 @@ //*************************************************************************************************************** -SeqSummaryCommand::SeqSummaryCommand(string option){ +SeqSummaryCommand::SeqSummaryCommand(string option) { try { abort = false; @@ -21,28 +21,53 @@ SeqSummaryCommand::SeqSummaryCommand(string option){ else { //valid paramters for this command - string Array[] = {"fasta"}; + string Array[] = {"fasta","processors","outputdir","inputdir"}; vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); OptionParser parser(option); map parameters = parser.getParameters(); ValidParameters validParameter; + map::iterator it; //check to make sure all parameters are valid for command - for (map::iterator it = parameters.begin(); it != parameters.end(); it++) { + for (it = parameters.begin(); it != parameters.end(); it++) { if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; } } + //if the user changes the input directory command factory will send this info to us in the output parameter + string inputDir = validParameter.validFile(parameters, "inputdir", false); + if (inputDir == "not found"){ inputDir = ""; } + else { + string path; + it = parameters.find("fasta"); + //user has given a template file + if(it != parameters.end()){ + path = hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["fasta"] = inputDir + it->second; } + } + } + //check for required parameters fastafile = validParameter.validFile(parameters, "fasta", true); if (fastafile == "not open") { abort = true; } - else if (fastafile == "not found") { fastafile = ""; mothurOut("fasta is a required parameter for the summary.seqs command."); mothurOutEndLine(); abort = true; } + else if (fastafile == "not found") { fastafile = ""; m->mothurOut("fasta is a required parameter for the summary.seqs command."); m->mothurOutEndLine(); abort = true; } + + //if the user changes the output directory command factory will send this info to us in the output parameter + outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ + outputDir = ""; + outputDir += hasPath(fastafile); //if user entered a file with a path then preserve it + } + string temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found"){ temp = "1"; } + convert(temp, processors); + + } } catch(exception& e) { - errorOut(e, "SeqSummaryCommand", "SeqSummaryCommand"); + m->errorOut(e, "SeqSummaryCommand", "SeqSummaryCommand"); exit(1); } } @@ -50,14 +75,14 @@ SeqSummaryCommand::SeqSummaryCommand(string option){ void SeqSummaryCommand::help(){ try { - mothurOut("The summary.seqs command reads a fastafile and ....\n"); - mothurOut("The summary.seqs command parameter is fasta and it is required.\n"); - mothurOut("The summary.seqs command should be in the following format: \n"); - mothurOut("summary.seqs(fasta=yourFastaFile) \n"); - mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n"); + m->mothurOut("The summary.seqs command reads a fastafile and summarizes the sequences.\n"); + m->mothurOut("The summary.seqs command parameters are fasta and processors, fasta is required.\n"); + m->mothurOut("The summary.seqs command should be in the following format: \n"); + m->mothurOut("summary.seqs(fasta=yourFastaFile, processors=2) \n"); + m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n"); } catch(exception& e) { - errorOut(e, "SeqSummaryCommand", "help"); + m->errorOut(e, "SeqSummaryCommand", "help"); exit(1); } } @@ -73,39 +98,171 @@ int SeqSummaryCommand::execute(){ if (abort == true) { return 0; } - ifstream inFASTA; - openInputFile(fastafile, inFASTA); + string summaryFile = outputDir + getSimpleName(fastafile) + ".summary"; + int numSeqs = 0; - - ofstream outSummary; - string summaryFile = fastafile + ".summary"; - openOutputFile(summaryFile, outSummary); vector startPosition; vector endPosition; vector seqLength; vector ambigBases; vector longHomoPolymer; + +#ifdef USE_MPI + int pid, numSeqsPerProcessor; + int tag = 2001; + int startTag = 1; int endTag = 2; int lengthTag = 3; int baseTag = 4; int lhomoTag = 5; + int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY; + vector MPIPos; + + MPI_Status status; + MPI_Status statusOut; + MPI_File inMPI; + MPI_File outMPI; + MPI_Comm_size(MPI_COMM_WORLD, &processors); + MPI_Comm_rank(MPI_COMM_WORLD, &pid); + + char tempFileName[1024]; + strcpy(tempFileName, fastafile.c_str()); + + char sumFileName[1024]; + strcpy(sumFileName, summaryFile.c_str()); - outSummary << "seqname\tstart\tend\tnbases\tambigs\tpolymer" << endl; + MPI_File_open(MPI_COMM_WORLD, tempFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer + MPI_File_open(MPI_COMM_WORLD, sumFileName, outMode, MPI_INFO_NULL, &outMPI); + + if (m->control_pressed) { MPI_File_close(&inMPI); MPI_File_close(&outMPI); return 0; } + + if (pid == 0) { //you are the root process + //print header + string outputString = "seqname\tstart\tend\tnbases\tambigs\tpolymer\n"; + int length = outputString.length(); + char* buf2 = new char[length]; + memcpy(buf2, outputString.c_str(), length); + + MPI_File_write_shared(outMPI, buf2, length, MPI_CHAR, &statusOut); + delete buf2; + + MPIPos = setFilePosFasta(fastafile, numSeqs); //fills MPIPos, returns numSeqs + + for(int i = 1; i < processors; i++) { + MPI_Send(&numSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD); + MPI_Send(&MPIPos[0], (numSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD); + } + + //figure out how many sequences you have to do + numSeqsPerProcessor = numSeqs / processors; + int startIndex = pid * numSeqsPerProcessor; + if(pid == (processors - 1)){ numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor; } + + //do your part + MPICreateSummary(startIndex, numSeqsPerProcessor, startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, inMPI, outMPI, MPIPos); + + }else { //i am the child process + + MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status); + MPIPos.resize(numSeqs+1); + MPI_Recv(&MPIPos[0], (numSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status); + + //figure out how many sequences you have to align + numSeqsPerProcessor = numSeqs / processors; + int startIndex = pid * numSeqsPerProcessor; + if(pid == (processors - 1)){ numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor; } + + //do your part + MPICreateSummary(startIndex, numSeqsPerProcessor, startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, inMPI, outMPI, MPIPos); + } + + MPI_File_close(&inMPI); + MPI_File_close(&outMPI); + MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case + + if (pid == 0) { + //get the info from the child processes + for(int i = 1; i < processors; i++) { + int size; + MPI_Recv(&size, 1, MPI_INT, i, tag, MPI_COMM_WORLD, &status); - while(!inFASTA.eof()){ - Sequence current(inFASTA); - startPosition.push_back(current.getStartPos()); - endPosition.push_back(current.getEndPos()); - seqLength.push_back(current.getNumBases()); - ambigBases.push_back(current.getAmbigBases()); - longHomoPolymer.push_back(current.getLongHomoPolymer()); + vector temp; temp.resize(size+1); + + for(int j = 0; j < 5; j++) { + + MPI_Recv(&temp[0], (size+1), MPI_INT, i, 2001, MPI_COMM_WORLD, &status); + int receiveTag = temp[temp.size()-1]; //child process added a int to the end to indicate what count this is for + + if (receiveTag == startTag) { + for (int k = 0; k < size; k++) { startPosition.push_back(temp[k]); } + }else if (receiveTag == endTag) { + for (int k = 0; k < size; k++) { endPosition.push_back(temp[k]); } + }else if (receiveTag == lengthTag) { + for (int k = 0; k < size; k++) { seqLength.push_back(temp[k]); } + }else if (receiveTag == baseTag) { + for (int k = 0; k < size; k++) { ambigBases.push_back(temp[k]); } + }else if (receiveTag == lhomoTag) { + for (int k = 0; k < size; k++) { longHomoPolymer.push_back(temp[k]); } + } + } + } - outSummary << current.getName() << '\t'; - outSummary << current.getStartPos() << '\t' << current.getEndPos() << '\t'; - outSummary << current.getNumBases() << '\t' << current.getAmbigBases() << '\t'; - outSummary << current.getLongHomoPolymer() << endl; + }else{ + + //send my counts + int size = startPosition.size(); + MPI_Send(&size, 1, MPI_INT, 0, tag, MPI_COMM_WORLD); + + startPosition.push_back(startTag); + int ierr = MPI_Send(&(startPosition[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + endPosition.push_back(endTag); + ierr = MPI_Send (&(endPosition[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + seqLength.push_back(lengthTag); + ierr = MPI_Send(&(seqLength[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + ambigBases.push_back(baseTag); + ierr = MPI_Send(&(ambigBases[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + longHomoPolymer.push_back(lhomoTag); + ierr = MPI_Send(&(longHomoPolymer[0]), (size+1), MPI_INT, 0, 2001, MPI_COMM_WORLD); + } + + MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case +#else + #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) + if(processors == 1){ + ifstream inFASTA; + openInputFile(fastafile, inFASTA); + getNumSeqs(inFASTA, numSeqs); + inFASTA.close(); + + lines.push_back(new linePair(0, numSeqs)); + + driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile, lines[0]); + }else{ + numSeqs = setLines(fastafile); + createProcessesCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile); + + rename((summaryFile + toString(processIDS[0]) + ".temp").c_str(), summaryFile.c_str()); + //append files + for(int i=1;icontrol_pressed) { return 0; } + #else + ifstream inFASTA; + openInputFile(fastafile, inFASTA); + getNumSeqs(inFASTA, numSeqs); + inFASTA.close(); + + lines.push_back(new linePair(0, numSeqs)); + + driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile, lines[0]); + if (m->control_pressed) { return 0; } + #endif +#endif - numSeqs++; - gobble(inFASTA); - } - inFASTA.close(); + #ifdef USE_MPI + if (pid == 0) { + #endif sort(startPosition.begin(), startPosition.end()); sort(endPosition.begin(), endPosition.end()); @@ -120,26 +277,231 @@ int SeqSummaryCommand::execute(){ int ptile97_5 = int(numSeqs * 0.975); int ptile100 = numSeqs - 1; - mothurOutEndLine(); - mothurOut("\t\tStart\tEnd\tNBases\tAmbigs\tPolymer"); mothurOutEndLine(); - mothurOut("Minimum:\t" + toString(startPosition[0]) + "\t" + toString(endPosition[0]) + "\t" + toString(seqLength[0]) + "\t" + toString(ambigBases[0]) + "\t" + toString(longHomoPolymer[0])); mothurOutEndLine(); - mothurOut("2.5%-tile:\t" + toString(startPosition[ptile0_25]) + "\t" + toString(endPosition[ptile0_25]) + "\t" + toString(seqLength[ptile0_25]) + "\t" + toString(ambigBases[ptile0_25]) + "\t"+ toString(longHomoPolymer[ptile0_25])); mothurOutEndLine(); - mothurOut("25%-tile:\t" + toString(startPosition[ptile25]) + "\t" + toString(endPosition[ptile25]) + "\t" + toString(seqLength[ptile25]) + "\t" + toString(ambigBases[ptile25]) + "\t" + toString(longHomoPolymer[ptile25])); mothurOutEndLine(); - mothurOut("Median: \t" + toString(startPosition[ptile50]) + "\t" + toString(endPosition[ptile50]) + "\t" + toString(seqLength[ptile50]) + "\t" + toString(ambigBases[ptile50]) + "\t" + toString(longHomoPolymer[ptile50])); mothurOutEndLine(); - mothurOut("75%-tile:\t" + toString(startPosition[ptile75]) + "\t" + toString(endPosition[ptile75]) + "\t" + toString(seqLength[ptile75]) + "\t" + toString(ambigBases[ptile75]) + "\t" + toString(longHomoPolymer[ptile75])); mothurOutEndLine(); - mothurOut("97.5%-tile:\t" + toString(startPosition[ptile97_5]) + "\t" + toString(endPosition[ptile97_5]) + "\t" + toString(seqLength[ptile97_5]) + "\t" + toString(ambigBases[ptile97_5]) + "\t" + toString(longHomoPolymer[ptile97_5])); mothurOutEndLine(); - mothurOut("Maximum:\t" + toString(startPosition[ptile100]) + "\t" + toString(endPosition[ptile100]) + "\t" + toString(seqLength[ptile100]) + "\t" + toString(ambigBases[ptile100]) + "\t" + toString(longHomoPolymer[ptile100])); mothurOutEndLine(); - mothurOut("# of Seqs:\t" + toString(numSeqs)); mothurOutEndLine(); - - outSummary.close(); + //to compensate for blank sequences that would result in startPosition and endPostion equalling -1 + if (startPosition[0] == -1) { startPosition[0] = 0; } + if (endPosition[0] == -1) { endPosition[0] = 0; } + + if (m->control_pressed) { remove(summaryFile.c_str()); return 0; } + + m->mothurOutEndLine(); + m->mothurOut("\t\tStart\tEnd\tNBases\tAmbigs\tPolymer"); m->mothurOutEndLine(); + m->mothurOut("Minimum:\t" + toString(startPosition[0]) + "\t" + toString(endPosition[0]) + "\t" + toString(seqLength[0]) + "\t" + toString(ambigBases[0]) + "\t" + toString(longHomoPolymer[0])); m->mothurOutEndLine(); + m->mothurOut("2.5%-tile:\t" + toString(startPosition[ptile0_25]) + "\t" + toString(endPosition[ptile0_25]) + "\t" + toString(seqLength[ptile0_25]) + "\t" + toString(ambigBases[ptile0_25]) + "\t"+ toString(longHomoPolymer[ptile0_25])); m->mothurOutEndLine(); + m->mothurOut("25%-tile:\t" + toString(startPosition[ptile25]) + "\t" + toString(endPosition[ptile25]) + "\t" + toString(seqLength[ptile25]) + "\t" + toString(ambigBases[ptile25]) + "\t" + toString(longHomoPolymer[ptile25])); m->mothurOutEndLine(); + m->mothurOut("Median: \t" + toString(startPosition[ptile50]) + "\t" + toString(endPosition[ptile50]) + "\t" + toString(seqLength[ptile50]) + "\t" + toString(ambigBases[ptile50]) + "\t" + toString(longHomoPolymer[ptile50])); m->mothurOutEndLine(); + m->mothurOut("75%-tile:\t" + toString(startPosition[ptile75]) + "\t" + toString(endPosition[ptile75]) + "\t" + toString(seqLength[ptile75]) + "\t" + toString(ambigBases[ptile75]) + "\t" + toString(longHomoPolymer[ptile75])); m->mothurOutEndLine(); + m->mothurOut("97.5%-tile:\t" + toString(startPosition[ptile97_5]) + "\t" + toString(endPosition[ptile97_5]) + "\t" + toString(seqLength[ptile97_5]) + "\t" + toString(ambigBases[ptile97_5]) + "\t" + toString(longHomoPolymer[ptile97_5])); m->mothurOutEndLine(); + m->mothurOut("Maximum:\t" + toString(startPosition[ptile100]) + "\t" + toString(endPosition[ptile100]) + "\t" + toString(seqLength[ptile100]) + "\t" + toString(ambigBases[ptile100]) + "\t" + toString(longHomoPolymer[ptile100])); m->mothurOutEndLine(); + m->mothurOut("# of Seqs:\t" + toString(numSeqs)); m->mothurOutEndLine(); + + if (m->control_pressed) { remove(summaryFile.c_str()); return 0; } + + m->mothurOutEndLine(); + m->mothurOut("Output File Name: "); m->mothurOutEndLine(); + m->mothurOut(summaryFile); m->mothurOutEndLine(); + m->mothurOutEndLine(); + + #ifdef USE_MPI + } + #endif + + return 0; + } + catch(exception& e) { + m->errorOut(e, "SeqSummaryCommand", "execute"); + exit(1); + } +} +/**************************************************************************************/ +int SeqSummaryCommand::driverCreateSummary(vector& startPosition, vector& endPosition, vector& seqLength, vector& ambigBases, vector& longHomoPolymer, string filename, string sumFile, linePair* line) { + try { + + ofstream outSummary; + openOutputFile(sumFile, outSummary); + + //print header if you are process 0 + if (line->start == 0) { + outSummary << "seqname\tstart\tend\tnbases\tambigs\tpolymer" << endl; + } + + ifstream in; + openInputFile(filename, in); + + in.seekg(line->start); + + for(int i=0;inum;i++){ + + if (m->control_pressed) { in.close(); outSummary.close(); return 1; } + + Sequence current(in); + + if (current.getName() != "") { + startPosition.push_back(current.getStartPos()); + endPosition.push_back(current.getEndPos()); + seqLength.push_back(current.getNumBases()); + ambigBases.push_back(current.getAmbigBases()); + longHomoPolymer.push_back(current.getLongHomoPolymer()); + + outSummary << current.getName() << '\t'; + outSummary << current.getStartPos() << '\t' << current.getEndPos() << '\t'; + outSummary << current.getNumBases() << '\t' << current.getAmbigBases() << '\t'; + outSummary << current.getLongHomoPolymer() << endl; + } + gobble(in); + } + in.close(); + return 0; } catch(exception& e) { - errorOut(e, "SeqSummaryCommand", "execute"); + m->errorOut(e, "SeqSummaryCommand", "driverCreateSummary"); exit(1); } } +#ifdef USE_MPI +/**************************************************************************************/ +int SeqSummaryCommand::MPICreateSummary(int start, int num, vector& startPosition, vector& endPosition, vector& seqLength, vector& ambigBases, vector& longHomoPolymer, MPI_File& inMPI, MPI_File& outMPI, vector& MPIPos) { + try { + + int pid; + MPI_Status status; + MPI_Comm_rank(MPI_COMM_WORLD, &pid); + + for(int i=0;icontrol_pressed) { return 0; } + + //read next sequence + int length = MPIPos[start+i+1] - MPIPos[start+i]; + + char* buf4 = new char[length]; + MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status); + + string tempBuf = buf4; + if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); } + istringstream iss (tempBuf,istringstream::in); + delete buf4; + + Sequence current(iss); + + if (current.getName() != "") { + startPosition.push_back(current.getStartPos()); + endPosition.push_back(current.getEndPos()); + seqLength.push_back(current.getNumBases()); + ambigBases.push_back(current.getAmbigBases()); + longHomoPolymer.push_back(current.getLongHomoPolymer()); + + string outputString = current.getName() + "\t" + toString(current.getStartPos()) + "\t" + toString(current.getEndPos()) + "\t"; + outputString += toString(current.getNumBases()) + "\t" + toString(current.getAmbigBases()) + "\t" + toString(current.getLongHomoPolymer()) + "\n"; + + //output to file + length = outputString.length(); + char* buf3 = new char[length]; + memcpy(buf3, outputString.c_str(), length); + + MPI_File_write_shared(outMPI, buf3, length, MPI_CHAR, &status); + delete buf3; + } + } + + return 0; + } + catch(exception& e) { + m->errorOut(e, "SeqSummaryCommand", "MPICreateSummary"); + exit(1); + } +} +#endif +/**************************************************************************************************/ +int SeqSummaryCommand::createProcessesCreateSummary(vector& startPosition, vector& endPosition, vector& seqLength, vector& ambigBases, vector& longHomoPolymer, string filename, string sumFile) { + try { +#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) + int process = 0; + int exitCommand = 1; + processIDS.clear(); + + //loop through and create all the processes you want + while (process != processors) { + int pid = vfork(); + + if (pid > 0) { + processIDS.push_back(pid); //create map from line number to pid so you can append files in correct order later + process++; + }else if (pid == 0){ + driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, sumFile + toString(getpid()) + ".temp", lines[process]); + exit(0); + }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); } + } + + //force parent to wait until all the processes are done + for (int i=0;ierrorOut(e, "SeqSummaryCommand", "createProcessesCreateSummary"); + exit(1); + } +} +/**************************************************************************************************/ + +int SeqSummaryCommand::setLines(string filename) { + try { + + vector positions; + + ifstream inFASTA; + openInputFile(filename, inFASTA); + + string input; + while(!inFASTA.eof()){ + input = getline(inFASTA); + + if (input.length() != 0) { + if(input[0] == '>'){ long int pos = inFASTA.tellg(); positions.push_back(pos - input.length() - 1); } + } + } + inFASTA.close(); + + int numFastaSeqs = positions.size(); + + FILE * pFile; + long size; + + //get num bytes in file + pFile = fopen (filename.c_str(),"rb"); + if (pFile==NULL) perror ("Error opening file"); + else{ + fseek (pFile, 0, SEEK_END); + size=ftell (pFile); + fclose (pFile); + } + + int numSeqsPerProcessor = numFastaSeqs / processors; + + for (int i = 0; i < processors; i++) { + + long int startPos = positions[ i * numSeqsPerProcessor ]; + if(i == processors - 1){ + numSeqsPerProcessor = numFastaSeqs - i * numSeqsPerProcessor; + }else{ + long int myEnd = positions[ (i+1) * numSeqsPerProcessor ]; + } + lines.push_back(new linePair(startPos, numSeqsPerProcessor)); + } + + return numFastaSeqs; + } + catch(exception& e) { + m->errorOut(e, "SeqSummaryCommand", "setLines"); + exit(1); + } +} //***************************************************************************************************************