X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=samtools.1;h=ba323926d83451cf4013c511cf832d6e820a76f9;hb=8ef16f0ffefe94ead2c9e367ebcb66490fdea2cb;hp=8d299b78dc079b6961e14137324ecad32e4b66d0;hpb=7251efa37992752a8cf62ff363da0ad5099937bd;p=samtools.git diff --git a/samtools.1 b/samtools.1 index 8d299b7..ba32392 100644 --- a/samtools.1 +++ b/samtools.1 @@ -1,4 +1,4 @@ -.TH samtools 1 "2 December 2010" "samtools-0.1.12" "Bioinformatics tools" +.TH samtools 1 "16 March 2011" "samtools-0.1.14" "Bioinformatics tools" .SH NAME .PP samtools - Utilities for the Sequence Alignment/Map (SAM) format @@ -145,7 +145,10 @@ identifiers are absent, each input file is regarded as one sample. .B OPTIONS: .RS -.TP 8 +.TP 10 +.B -A +Do not skip anomalous read pairs in variant calling. +.TP .B -B Disable probabilistic realignment for the computation of base alignment quality (BAQ). BAQ is the Phred-scaled probability of a read base being @@ -159,6 +162,14 @@ being generated from the mapped position, the new mapping quality is about sqrt((INT-q)/INT)*INT. A zero value disables this functionality; if enabled, the recommended value for BWA is 50. [0] .TP +.BI -d \ INT +At a position, read maximally +.I INT +reads per input BAM. [250] +.TP +.B -D +Output per-sample read depth +.TP .BI -e \ INT Phred-scaled gap extension sequencing error probability. Reducing .I INT @@ -184,9 +195,17 @@ is modeled as .IR INT * s / l . [100] .TP +.B -I +Do not perform INDEL calling +.TP .BI -l \ FILE File containing a list of sites where pileup or BCF is outputted [null] .TP +.BI -L \ INT +Skip INDEL calling if the average per-sample depth is above +.IR INT . +[250] +.TP .BI -o \ INT Phred-scaled gap open sequencing error probability. Reducing .I INT @@ -210,6 +229,9 @@ Only generate pileup in region .I STR [all sites] .TP +.B -S +Output per-sample Phred-scaled strand bias P-value +.TP .B -u Similar to .B -g @@ -227,6 +249,16 @@ with the header in This command is much faster than replacing the header with a BAM->SAM->BAM conversion. +.TP +.B cat +samtools cat [-h header.sam] [-o out.bam] [ ... ] + +Concatenate BAMs. The sequence dictionary of each input BAM must be identical, +although this command does not check this. This command uses a similar trick +to +.B reheader +which enables fast BAM concatenation. + .TP .B sort samtools sort [-no] [-m maxMem] @@ -414,12 +446,15 @@ designed for cutting fosmid clones from fosmid pool sequencing [Ref. Kitzman et .TP .B phase -samtools phase [-F] [-k len] [-b prefix] [-q minLOD] [-Q minBaseQ] +samtools phase [-AF] [-k len] [-b prefix] [-q minLOD] [-Q minBaseQ] Call and phase heterozygous SNPs. .B OPTIONS: .RS .TP 8 +.B -A +Drop reads with ambiguous phase. +.TP 8 .BI -b \ STR Prefix of BAM output. When this option is in use, phase-0 reads will be saved in file .BR STR .0.bam