X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=getseqscommand.cpp;h=ccabafb6d19c008eb6957a35e79b5cd0623021d0;hb=8e67e9de1b200106bea5a468ac02125954656499;hp=4b93d1fdeea1aac9048013d755ceda466e5f37a1;hpb=3aa0b70bf057656ccc2bae1de9816583f2a91779;p=mothur.git diff --git a/getseqscommand.cpp b/getseqscommand.cpp index 4b93d1f..ccabafb 100644 --- a/getseqscommand.cpp +++ b/getseqscommand.cpp @@ -12,22 +12,54 @@ #include "listvector.hpp" //********************************************************************************************************************** -vector GetSeqsCommand::getValidParameters(){ +vector GetSeqsCommand::setParameters(){ try { - string Array[] = {"fasta","name", "group", "alignreport", "accnos", "dups", "list","taxonomy","outputdir","inputdir"}; - vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); + CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pfasta); + CommandParameter pname("name", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pname); + CommandParameter pgroup("group", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pgroup); + CommandParameter plist("list", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(plist); + CommandParameter ptaxonomy("taxonomy", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(ptaxonomy); + CommandParameter palignreport("alignreport", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(palignreport); + CommandParameter pqfile("qfile", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pqfile); + CommandParameter paccnos("accnos", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(paccnos); + CommandParameter pdups("dups", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(pdups); + CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir); + CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir); + CommandParameter paccnos2("accnos2", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(paccnos2); + + vector myArray; + for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); } return myArray; } catch(exception& e) { - m->errorOut(e, "GetSeqsCommand", "getValidParameters"); + m->errorOut(e, "GetSeqsCommand", "setParameters"); exit(1); } } +//********************************************************************************************************************** +string GetSeqsCommand::getHelpString(){ + try { + string helpString = ""; + helpString += "The get.seqs command reads an .accnos file and any of the following file types: fasta, name, group, list, taxonomy, quality or alignreport file.\n"; + helpString += "It outputs a file containing only the sequences in the .accnos file.\n"; + helpString += "The get.seqs command parameters are accnos, fasta, name, group, list, taxonomy, qfile, alignreport and dups. You must provide accnos unless you have a valid current accnos file, and at least one of the other parameters.\n"; + helpString += "The dups parameter allows you to add the entire line from a name file if you add any name from the line. default=false. \n"; + helpString += "The get.seqs command should be in the following format: get.seqs(accnos=yourAccnos, fasta=yourFasta).\n"; + helpString += "Example get.seqs(accnos=amazon.accnos, fasta=amazon.fasta).\n"; + helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n"; + return helpString; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "getHelpString"); + exit(1); + } +} + //********************************************************************************************************************** GetSeqsCommand::GetSeqsCommand(){ try { - abort = true; - //initialize outputTypes + abort = true; calledHelp = true; + setParameters(); vector tempOutNames; outputTypes["fasta"] = tempOutNames; outputTypes["taxonomy"] = tempOutNames; @@ -35,6 +67,8 @@ GetSeqsCommand::GetSeqsCommand(){ outputTypes["group"] = tempOutNames; outputTypes["alignreport"] = tempOutNames; outputTypes["list"] = tempOutNames; + outputTypes["qfile"] = tempOutNames; + outputTypes["accnosreport"] = tempOutNames; } catch(exception& e) { m->errorOut(e, "GetSeqsCommand", "GetSeqsCommand"); @@ -42,40 +76,43 @@ GetSeqsCommand::GetSeqsCommand(){ } } //********************************************************************************************************************** -vector GetSeqsCommand::getRequiredParameters(){ +string GetSeqsCommand::getOutputFileNameTag(string type, string inputName=""){ try { - string Array[] = {"accnos"}; - vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); - return myArray; + string outputFileName = ""; + map >::iterator it; + + //is this a type this command creates + it = outputTypes.find(type); + if (it == outputTypes.end()) { m->mothurOut("[ERROR]: this command doesn't create a " + type + " output file.\n"); } + else { + if (type == "fasta") { outputFileName = "pick" + m->getExtension(inputName); } + else if (type == "taxonomy") { outputFileName = "pick" + m->getExtension(inputName); } + else if (type == "name") { outputFileName = "pick" + m->getExtension(inputName); } + else if (type == "group") { outputFileName = "pick" + m->getExtension(inputName); } + else if (type == "list") { outputFileName = "pick" + m->getExtension(inputName); } + else if (type == "qfile") { outputFileName = "pick" + m->getExtension(inputName); } + else if (type == "accnosreport"){ outputFileName = "accnos.report"; } + else if (type == "alignreport") { outputFileName = "pick.align.report"; } + else { m->mothurOut("[ERROR]: No definition for type " + type + " output file tag.\n"); m->control_pressed = true; } + } + return outputFileName; } catch(exception& e) { - m->errorOut(e, "GetSeqsCommand", "getRequiredParameters"); - exit(1); - } -} -//********************************************************************************************************************** -vector GetSeqsCommand::getRequiredFiles(){ - try { - vector myArray; - return myArray; - } - catch(exception& e) { - m->errorOut(e, "GetSeqsCommand", "getRequiredFiles"); + m->errorOut(e, "GetSeqsCommand", "getOutputFileNameTag"); exit(1); } } //********************************************************************************************************************** GetSeqsCommand::GetSeqsCommand(string option) { try { - abort = false; + abort = false; calledHelp = false; //allow user to run help - if(option == "help") { help(); abort = true; } + if(option == "help") { help(); abort = true; calledHelp = true; } + else if(option == "citation") { citation(); abort = true; calledHelp = true;} else { - //valid paramters for this command - string Array[] = {"fasta","name", "group", "alignreport", "accnos", "dups", "list","taxonomy","outputdir","inputdir"}; - vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); + vector myArray = setParameters(); OptionParser parser(option); map parameters = parser.getParameters(); @@ -96,6 +133,8 @@ GetSeqsCommand::GetSeqsCommand(string option) { outputTypes["group"] = tempOutNames; outputTypes["alignreport"] = tempOutNames; outputTypes["list"] = tempOutNames; + outputTypes["qfile"] = tempOutNames; + outputTypes["accnosreport"] = tempOutNames; //if the user changes the output directory command factory will send this info to us in the output parameter outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; } @@ -129,6 +168,14 @@ GetSeqsCommand::GetSeqsCommand(string option) { if (path == "") { parameters["accnos"] = inputDir + it->second; } } + it = parameters.find("accnos2"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["accnos2"] = inputDir + it->second; } + } + it = parameters.find("list"); //user has given a template file if(it != parameters.end()){ @@ -160,25 +207,45 @@ GetSeqsCommand::GetSeqsCommand(string option) { //if the user has not given a path then, add inputdir. else leave path alone. if (path == "") { parameters["taxonomy"] = inputDir + it->second; } } + + it = parameters.find("qfile"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["qfile"] = inputDir + it->second; } + } } //check for required parameters accnosfile = validParameter.validFile(parameters, "accnos", true); if (accnosfile == "not open") { abort = true; } - else if (accnosfile == "not found") { accnosfile = ""; m->mothurOut("You must provide an accnos file."); m->mothurOutEndLine(); abort = true; } + else if (accnosfile == "not found") { + accnosfile = m->getAccnosFile(); + if (accnosfile != "") { m->mothurOut("Using " + accnosfile + " as input file for the accnos parameter."); m->mothurOutEndLine(); } + else { + m->mothurOut("You have no valid accnos file and accnos is required."); m->mothurOutEndLine(); + abort = true; + } + }else { m->setAccnosFile(accnosfile); } + + if (accnosfile2 == "not found") { accnosfile2 = ""; } fastafile = validParameter.validFile(parameters, "fasta", true); - if (fastafile == "not open") { abort = true; } - else if (fastafile == "not found") { fastafile = ""; } + if (fastafile == "not open") { fastafile = ""; abort = true; } + else if (fastafile == "not found") { fastafile = ""; } + else { m->setFastaFile(fastafile); } namefile = validParameter.validFile(parameters, "name", true); - if (namefile == "not open") { abort = true; } + if (namefile == "not open") { namefile = ""; abort = true; } else if (namefile == "not found") { namefile = ""; } + else { m->setNameFile(namefile); } groupfile = validParameter.validFile(parameters, "group", true); if (groupfile == "not open") { abort = true; } else if (groupfile == "not found") { groupfile = ""; } + else { m->setGroupFile(groupfile); } alignfile = validParameter.validFile(parameters, "alignreport", true); if (alignfile == "not open") { abort = true; } @@ -187,19 +254,33 @@ GetSeqsCommand::GetSeqsCommand(string option) { listfile = validParameter.validFile(parameters, "list", true); if (listfile == "not open") { abort = true; } else if (listfile == "not found") { listfile = ""; } + else { m->setListFile(listfile); } taxfile = validParameter.validFile(parameters, "taxonomy", true); - if (taxfile == "not open") { abort = true; } + if (taxfile == "not open") { taxfile = ""; abort = true; } else if (taxfile == "not found") { taxfile = ""; } + else { m->setTaxonomyFile(taxfile); } + + qualfile = validParameter.validFile(parameters, "qfile", true); + if (qualfile == "not open") { abort = true; } + else if (qualfile == "not found") { qualfile = ""; } + else { m->setQualFile(qualfile); } + + accnosfile2 = validParameter.validFile(parameters, "accnos2", true); + if (accnosfile2 == "not open") { abort = true; } + else if (accnosfile2 == "not found") { accnosfile2 = ""; } + string usedDups = "true"; - string temp = validParameter.validFile(parameters, "dups", false); if (temp == "not found") { temp = "false"; usedDups = ""; } + string temp = validParameter.validFile(parameters, "dups", false); if (temp == "not found") { temp = "true"; usedDups = ""; } dups = m->isTrue(temp); - if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "")) { m->mothurOut("You must provide one of the following: fasta, name, group, alignreport, taxonomy or listfile."); m->mothurOutEndLine(); abort = true; } + if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "") && (qualfile == "") && (accnosfile2 == "")) { m->mothurOut("You must provide one of the following: fasta, name, group, alignreport, taxonomy, quality or listfile."); m->mothurOutEndLine(); abort = true; } - if ((usedDups != "") && (namefile == "")) { m->mothurOut("You may only use dups with the name option."); m->mothurOutEndLine(); abort = true; } - + if ((namefile == "") && ((fastafile != "") || (taxfile != ""))){ + vector files; files.push_back(fastafile); files.push_back(taxfile); + parser.getNameFile(files); + } } } @@ -210,49 +291,69 @@ GetSeqsCommand::GetSeqsCommand(string option) { } //********************************************************************************************************************** -void GetSeqsCommand::help(){ - try { - m->mothurOut("The get.seqs command reads an .accnos file and any of the following file types: fasta, name, group, list, taxonomy or alignreport file.\n"); - m->mothurOut("It outputs a file containing only the sequences in the .accnos file.\n"); - m->mothurOut("The get.seqs command parameters are accnos, fasta, name, group, list, taxonomy, alignreport and dups. You must provide accnos and at least one of the other parameters.\n"); - m->mothurOut("The dups parameter allows you to add the entire line from a name file if you add any name from the line. default=false. \n"); - m->mothurOut("The get.seqs command should be in the following format: get.seqs(accnos=yourAccnos, fasta=yourFasta).\n"); - m->mothurOut("Example get.seqs(accnos=amazon.accnos, fasta=amazon.fasta).\n"); - m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n"); - } - catch(exception& e) { - m->errorOut(e, "GetSeqsCommand", "help"); - exit(1); - } -} - -//********************************************************************************************************************** - int GetSeqsCommand::execute(){ try { - if (abort == true) { return 0; } + if (abort == true) { if (calledHelp) { return 0; } return 2; } //get names you want to keep - readAccnos(); + names = m->readAccnos(accnosfile); if (m->control_pressed) { return 0; } //read through the correct file and output lines you want to keep - if (namefile != "") { readName(); } - if (fastafile != "") { readFasta(); } - if (groupfile != "") { readGroup(); } - if (alignfile != "") { readAlign(); } - if (listfile != "") { readList(); } - if (taxfile != "") { readTax(); } + if (namefile != "") { readName(); } + if (fastafile != "") { readFasta(); } + if (groupfile != "") { readGroup(); } + if (alignfile != "") { readAlign(); } + if (listfile != "") { readList(); } + if (taxfile != "") { readTax(); } + if (qualfile != "") { readQual(); } + if (accnosfile2 != "") { compareAccnos(); } + + if (m->debug) { runSanityCheck(); } + + if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; } - if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; } if (outputNames.size() != 0) { m->mothurOutEndLine(); m->mothurOut("Output File Names: "); m->mothurOutEndLine(); for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); } m->mothurOutEndLine(); + + //set fasta file as new current fastafile + string current = ""; + itTypes = outputTypes.find("fasta"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); } + } + + itTypes = outputTypes.find("name"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setNameFile(current); } + } + + itTypes = outputTypes.find("group"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setGroupFile(current); } + } + + itTypes = outputTypes.find("list"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setListFile(current); } + } + + itTypes = outputTypes.find("taxonomy"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setTaxonomyFile(current); } + } + + itTypes = outputTypes.find("qfile"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setQualFile(current); } + } + } return 0; @@ -269,7 +370,7 @@ int GetSeqsCommand::readFasta(){ try { string thisOutputDir = outputDir; if (outputDir == "") { thisOutputDir += m->hasPath(fastafile); } - string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)) + "pick" + m->getExtension(fastafile); + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)) + getOutputFileNameTag("fasta", fastafile); ofstream out; m->openOutputFile(outputFileName, out); @@ -279,10 +380,13 @@ int GetSeqsCommand::readFasta(){ string name; bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["fasta"] = temp; } while(!in.eof()){ - if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str()); return 0; } + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } Sequence currSeq(in); name = currSeq.getName(); @@ -293,6 +397,9 @@ int GetSeqsCommand::readFasta(){ wroteSomething = true; currSeq.printSequence(out); + selectedCount++; + + if (m->debug) { sanity["fasta"].insert(name); } } } m->gobble(in); @@ -304,6 +411,8 @@ int GetSeqsCommand::readFasta(){ if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } outputNames.push_back(outputFileName); outputTypes["fasta"].push_back(outputFileName); + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your fasta file."); m->mothurOutEndLine(); + return 0; } @@ -313,11 +422,83 @@ int GetSeqsCommand::readFasta(){ } } //********************************************************************************************************************** +int GetSeqsCommand::readQual(){ + try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(qualfile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(qualfile)) + getOutputFileNameTag("qfile", qualfile); + ofstream out; + m->openOutputFile(outputFileName, out); + + + ifstream in; + m->openInputFile(qualfile, in); + string name; + + bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["qual"] = temp; } + + while(!in.eof()){ + string saveName = ""; + string name = ""; + string scores = ""; + + in >> name; + + if (name.length() != 0) { + saveName = name.substr(1); + while (!in.eof()) { + char c = in.get(); + if (c == 10 || c == 13){ break; } + else { name += c; } + } + m->gobble(in); + } + + while(in){ + char letter= in.get(); + if(letter == '>'){ in.putback(letter); break; } + else{ scores += letter; } + } + + m->gobble(in); + + if (names.count(saveName) != 0) { + wroteSomething = true; + + out << name << endl << scores; + selectedCount++; + if (m->debug) { sanity["qual"].insert(name); } + } + + m->gobble(in); + } + in.close(); + out.close(); + + + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputNames.push_back(outputFileName); outputTypes["qfile"].push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your quality file."); m->mothurOutEndLine(); + + + return 0; + + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "readQual"); + exit(1); + } +} +//********************************************************************************************************************** int GetSeqsCommand::readList(){ try { string thisOutputDir = outputDir; if (outputDir == "") { thisOutputDir += m->hasPath(listfile); } - string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(listfile)) + "pick" + m->getExtension(listfile); + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(listfile)) + getOutputFileNameTag("list", listfile); ofstream out; m->openOutputFile(outputFileName, out); @@ -325,10 +506,15 @@ int GetSeqsCommand::readList(){ m->openInputFile(listfile, in); bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["list"] = temp; } while(!in.eof()){ - if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str()); return 0; } + selectedCount = 0; + + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } //read in list vector ListVector list(in); @@ -349,11 +535,11 @@ int GetSeqsCommand::readList(){ binnames = binnames.substr(binnames.find_first_of(',')+1, binnames.length()); //if that name is in the .accnos file, add it - if (names.count(name) != 0) { newNames += name + ","; } + if (names.count(name) != 0) { newNames += name + ","; selectedCount++; if (m->debug) { sanity["list"].insert(name); } } } //get last name - if (names.count(binnames) != 0) { newNames += binnames + ","; } + if (names.count(binnames) != 0) { newNames += binnames + ","; selectedCount++; if (m->debug) { sanity["list"].insert(binnames); } } //if there are names in this bin add to new list if (newNames != "") { @@ -376,6 +562,8 @@ int GetSeqsCommand::readList(){ if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } outputNames.push_back(outputFileName); outputTypes["list"].push_back(outputFileName); + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your list file."); m->mothurOutEndLine(); + return 0; } @@ -389,7 +577,7 @@ int GetSeqsCommand::readName(){ try { string thisOutputDir = outputDir; if (outputDir == "") { thisOutputDir += m->hasPath(namefile); } - string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(namefile)) + "pick" + m->getExtension(namefile); + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(namefile)) + getOutputFileNameTag("name", namefile); ofstream out; m->openOutputFile(outputFileName, out); @@ -399,11 +587,14 @@ int GetSeqsCommand::readName(){ string name, firstCol, secondCol; bool wroteSomething = false; - + int selectedCount = 0; + + if (m->debug) { set temp; sanity["name"] = temp; } + if (m->debug) { set temp; sanity["dupname"] = temp; } while(!in.eof()){ - if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str()); return 0; } + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } in >> firstCol; in >> secondCol; @@ -412,28 +603,25 @@ int GetSeqsCommand::readName(){ if (dups) { hold = secondCol; } vector parsedNames; - //parse second column saving each name - while (secondCol.find_first_of(',') != -1) { - name = secondCol.substr(0,secondCol.find_first_of(',')); - secondCol = secondCol.substr(secondCol.find_first_of(',')+1, secondCol.length()); - parsedNames.push_back(name); - } - - //get name after last , - parsedNames.push_back(secondCol); + m->splitAtComma(secondCol, parsedNames); vector validSecond; for (int i = 0; i < parsedNames.size(); i++) { if (names.count(parsedNames[i]) != 0) { validSecond.push_back(parsedNames[i]); + if (m->debug) { sanity["dupname"].insert(parsedNames[i]); } } } if ((dups) && (validSecond.size() != 0)) { //dups = true and we want to add someone, then add everyone - for (int i = 0; i < parsedNames.size(); i++) { names.insert(parsedNames[i]); } + for (int i = 0; i < parsedNames.size(); i++) { names.insert(parsedNames[i]); if (m->debug) { sanity["dupname"].insert(parsedNames[i]); } } out << firstCol << '\t' << hold << endl; wroteSomething = true; + selectedCount += parsedNames.size(); + if (m->debug) { sanity["name"].insert(firstCol); } }else { + selectedCount += validSecond.size(); + //if the name in the first column is in the set then print it and any other names in second column also in set if (names.count(firstCol) != 0) { @@ -444,6 +632,8 @@ int GetSeqsCommand::readName(){ //you know you have at least one valid second since first column is valid for (int i = 0; i < validSecond.size()-1; i++) { out << validSecond[i] << ','; } out << validSecond[validSecond.size()-1] << endl; + + if (m->debug) { sanity["name"].insert(firstCol); } //make first name in set you come to first column and then add the remaining names to second column @@ -458,6 +648,8 @@ int GetSeqsCommand::readName(){ //you know you have at least one valid second since first column is valid for (int i = 0; i < validSecond.size()-1; i++) { out << validSecond[i] << ','; } out << validSecond[validSecond.size()-1] << endl; + + if (m->debug) { sanity["name"].insert(validSecond[0]); } } } } @@ -469,6 +661,8 @@ int GetSeqsCommand::readName(){ if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } outputNames.push_back(outputFileName); outputTypes["name"].push_back(outputFileName); + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your name file."); m->mothurOutEndLine(); + return 0; } @@ -483,7 +677,7 @@ int GetSeqsCommand::readGroup(){ try { string thisOutputDir = outputDir; if (outputDir == "") { thisOutputDir += m->hasPath(groupfile); } - string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(groupfile)) + "pick" + m->getExtension(groupfile); + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(groupfile)) + getOutputFileNameTag("group", groupfile); ofstream out; m->openOutputFile(outputFileName, out); @@ -493,10 +687,13 @@ int GetSeqsCommand::readGroup(){ string name, group; bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["group"] = temp; } while(!in.eof()){ - if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str()); return 0; } + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } in >> name; //read from first column @@ -507,6 +704,9 @@ int GetSeqsCommand::readGroup(){ wroteSomething = true; out << name << '\t' << group << endl; + selectedCount++; + + if (m->debug) { sanity["group"].insert(name); } } m->gobble(in); @@ -517,6 +717,9 @@ int GetSeqsCommand::readGroup(){ if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } outputNames.push_back(outputFileName); outputTypes["group"].push_back(outputFileName); + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your group file."); m->mothurOutEndLine(); + + return 0; } @@ -530,7 +733,7 @@ int GetSeqsCommand::readTax(){ try { string thisOutputDir = outputDir; if (outputDir == "") { thisOutputDir += m->hasPath(taxfile); } - string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(taxfile)) + "pick" + m->getExtension(taxfile); + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(taxfile)) + getOutputFileNameTag("taxonomy", taxfile); ofstream out; m->openOutputFile(outputFileName, out); @@ -539,10 +742,13 @@ int GetSeqsCommand::readTax(){ string name, tax; bool wroteSomething = false; + int selectedCount = 0; + + if (m->debug) { set temp; sanity["tax"] = temp; } while(!in.eof()){ - if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str()); return 0; } + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } in >> name; //read from first column in >> tax; //read from second column @@ -552,6 +758,9 @@ int GetSeqsCommand::readTax(){ wroteSomething = true; out << name << '\t' << tax << endl; + selectedCount++; + + if (m->debug) { sanity["tax"].insert(name); } } m->gobble(in); @@ -561,6 +770,8 @@ int GetSeqsCommand::readTax(){ if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } outputNames.push_back(outputFileName); outputTypes["taxonomy"].push_back(outputFileName); + + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your taxonomy file."); m->mothurOutEndLine(); return 0; @@ -576,7 +787,7 @@ int GetSeqsCommand::readAlign(){ try { string thisOutputDir = outputDir; if (outputDir == "") { thisOutputDir += m->hasPath(alignfile); } - string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(alignfile)) + "pick.align.report"; + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(alignfile)) + getOutputFileNameTag("alignreport"); ofstream out; m->openOutputFile(outputFileName, out); @@ -586,6 +797,7 @@ int GetSeqsCommand::readAlign(){ string name, junk; bool wroteSomething = false; + int selectedCount = 0; //read column headers for (int i = 0; i < 16; i++) { @@ -596,7 +808,7 @@ int GetSeqsCommand::readAlign(){ while(!in.eof()){ - if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str()); return 0; } + if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; } in >> name; //read from first column @@ -604,6 +816,7 @@ int GetSeqsCommand::readAlign(){ //if this name is in the accnos file if (names.count(name) != 0) { wroteSomething = true; + selectedCount++; out << name << '\t'; @@ -630,6 +843,8 @@ int GetSeqsCommand::readAlign(){ if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } outputNames.push_back(outputFileName); outputTypes["alignreport"].push_back(outputFileName); + m->mothurOut("Selected " + toString(selectedCount) + " sequences from your alignreport file."); m->mothurOutEndLine(); + return 0; } @@ -639,31 +854,203 @@ int GetSeqsCommand::readAlign(){ } } //********************************************************************************************************************** +//just looking at common mistakes. +int GetSeqsCommand::runSanityCheck(){ + try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(fastafile); } + string filename = outputDir + "get.seqs.debug.report"; + + ofstream out; + m->openOutputFile(filename, out); -int GetSeqsCommand::readAccnos(){ + + //compare fasta, name, qual and taxonomy if given to make sure they contain the same seqs + if (fastafile != "") { + if (namefile != "") { //compare with fasta + if (sanity["fasta"] != sanity["name"]) { //create mismatch file + createMisMatchFile(out, fastafile, namefile, sanity["fasta"], sanity["name"]); + } + } + if (qualfile != "") { + if (sanity["fasta"] != sanity["qual"]) { //create mismatch file + createMisMatchFile(out, fastafile, qualfile, sanity["fasta"], sanity["qual"]); + } + } + if (taxfile != "") { + if (sanity["fasta"] != sanity["tax"]) { //create mismatch file + createMisMatchFile(out, fastafile, taxfile, sanity["fasta"], sanity["tax"]); + } + } + } + + //compare dupnames, groups and list if given to make sure they match + if (namefile != "") { + if (groupfile != "") { + if (sanity["dupname"] != sanity["group"]) { //create mismatch file + createMisMatchFile(out, namefile, groupfile, sanity["dupname"], sanity["group"]); + } + } + if (listfile != "") { + if (sanity["dupname"] != sanity["list"]) { //create mismatch file + createMisMatchFile(out, namefile, listfile, sanity["dupname"], sanity["list"]); + } + } + }else{ + + if ((groupfile != "") && (fastafile != "")) { + if (sanity["fasta"] != sanity["group"]) { //create mismatch file + createMisMatchFile(out, fastafile, groupfile, sanity["fasta"], sanity["group"]); + } + } + } + + out.close(); + + if (m->isBlank(filename)) { m->mothurRemove(filename); } + else { m->mothurOut("\n[DEBUG]: " + filename + " contains the file mismatches.\n");outputNames.push_back(filename); outputTypes["debug"].push_back(filename); } + + return 0; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "runSanityCheck"); + exit(1); + } +} +//********************************************************************************************************************** +//just looking at common mistakes. +int GetSeqsCommand::createMisMatchFile(ofstream& out, string filename1, string filename2, set set1, set set2){ try { + out << "****************************************" << endl << endl; + out << "Names unique to " << filename1 << ":\n"; + + //remove names in set1 that are also in set2 + for (set::iterator it = set1.begin(); it != set1.end();) { + string name = *it; + + if (set2.count(name) == 0) { out << name << endl; } //name unique to set1 + else { set2.erase(name); } //you are in both so erase + set1.erase(it++); + } + + out << "\nNames unique to " << filename2 << ":\n"; + //output results + for (set::iterator it = set2.begin(); it != set2.end(); it++) { out << *it << endl; } + + out << "****************************************" << endl << endl; + + return 0; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "runSanityCheck"); + exit(1); + } +} +//********************************************************************************************************************** + +int GetSeqsCommand::compareAccnos(){ + try { + + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(accnosfile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(accnosfile)) + getOutputFileNameTag("accnosreport"); + ofstream out; + m->openOutputFile(outputFileName, out); ifstream in; - m->openInputFile(accnosfile, in); + m->openInputFile(accnosfile2, in); string name; + set namesAccnos2; + set namesDups; + set namesAccnos = names; + + map nameCount; + + if (namefile != "") { + ifstream inName; + m->openInputFile(namefile, inName); + + + while(!inName.eof()){ + + if (m->control_pressed) { inName.close(); return 0; } + + string thisname, repnames; + + inName >> thisname; m->gobble(inName); //read from first column + inName >> repnames; //read from second column + + int num = m->getNumNames(repnames); + nameCount[thisname] = num; + + m->gobble(inName); + } + inName.close(); + } + while(!in.eof()){ in >> name; - - names.insert(name); + + if (namesAccnos.count(name) == 0){ //name unique to accnos2 + int pos = name.find_last_of('_'); + string tempName = name; + if (pos != string::npos) { tempName = tempName.substr(pos+1); cout << tempName << endl; } + if (namesAccnos.count(tempName) == 0){ + namesAccnos2.insert(name); + }else { //you are in both so erase + namesAccnos.erase(name); + namesDups.insert(name); + } + }else { //you are in both so erase + namesAccnos.erase(name); + namesDups.insert(name); + } m->gobble(in); } in.close(); - return 0; + out << "Names in both files : " + toString(namesDups.size()) << endl; + m->mothurOut("Names in both files : " + toString(namesDups.size())); m->mothurOutEndLine(); + + for (set::iterator it = namesDups.begin(); it != namesDups.end(); it++) { + out << (*it); + if (namefile != "") { out << '\t' << nameCount[(*it)]; } + out << endl; + } + + out << "Names unique to " + accnosfile + " : " + toString(namesAccnos.size()) << endl; + m->mothurOut("Names unique to " + accnosfile + " : " + toString(namesAccnos.size())); m->mothurOutEndLine(); + + for (set::iterator it = namesAccnos.begin(); it != namesAccnos.end(); it++) { + out << (*it); + if (namefile != "") { out << '\t' << nameCount[(*it)]; } + out << endl; + } + + out << "Names unique to " + accnosfile2 + " : " + toString(namesAccnos2.size()) << endl; + m->mothurOut("Names unique to " + accnosfile2 + " : " + toString(namesAccnos2.size())); m->mothurOutEndLine(); + + for (set::iterator it = namesAccnos2.begin(); it != namesAccnos2.end(); it++) { + out << (*it); + if (namefile != "") { out << '\t' << nameCount[(*it)]; } + out << endl; + } + out.close(); + + outputNames.push_back(outputFileName); outputTypes["accnosreport"].push_back(outputFileName); + + return 0; + } catch(exception& e) { - m->errorOut(e, "GetSeqsCommand", "readAccnos"); + m->errorOut(e, "GetSeqsCommand", "compareAccnos"); exit(1); } } + //**********************************************************************************************************************