X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=getseqscommand.cpp;h=39c4fe001c8b9b19ef31c5a9a7f3ad98213301d6;hb=fd5f17bcbe5fadab2c525f3d1fcfd1d79a60ae72;hp=e7226ceca0b1e959040fbea6d78818ba23731a6e;hpb=260ae19c36cb11a53ddc5a75b5e507f8dd8b31d6;p=mothur.git diff --git a/getseqscommand.cpp b/getseqscommand.cpp index e7226ce..39c4fe0 100644 --- a/getseqscommand.cpp +++ b/getseqscommand.cpp @@ -12,7 +12,60 @@ #include "listvector.hpp" //********************************************************************************************************************** - +vector GetSeqsCommand::getValidParameters(){ + try { + string Array[] = {"fasta","name", "group", "qfile","alignreport", "accnos", "dups", "list","taxonomy","outputdir","inputdir"}; + vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); + return myArray; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "getValidParameters"); + exit(1); + } +} +//********************************************************************************************************************** +GetSeqsCommand::GetSeqsCommand(){ + try { + abort = true; + //initialize outputTypes + vector tempOutNames; + outputTypes["fasta"] = tempOutNames; + outputTypes["taxonomy"] = tempOutNames; + outputTypes["name"] = tempOutNames; + outputTypes["group"] = tempOutNames; + outputTypes["alignreport"] = tempOutNames; + outputTypes["list"] = tempOutNames; + outputTypes["qfile"] = tempOutNames; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "GetSeqsCommand"); + exit(1); + } +} +//********************************************************************************************************************** +vector GetSeqsCommand::getRequiredParameters(){ + try { + string Array[] = {"accnos"}; + vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); + return myArray; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "getRequiredParameters"); + exit(1); + } +} +//********************************************************************************************************************** +vector GetSeqsCommand::getRequiredFiles(){ + try { + vector myArray; + return myArray; + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "getRequiredFiles"); + exit(1); + } +} +//********************************************************************************************************************** GetSeqsCommand::GetSeqsCommand(string option) { try { abort = false; @@ -22,7 +75,7 @@ GetSeqsCommand::GetSeqsCommand(string option) { else { //valid paramters for this command - string Array[] = {"fasta","name", "group", "alignreport", "accnos", "dups", "list","taxonomy","outputdir","inputdir"}; + string Array[] = {"fasta","name", "group", "alignreport", "qfile", "accnos", "dups", "list","taxonomy","outputdir","inputdir"}; vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); OptionParser parser(option); @@ -36,6 +89,16 @@ GetSeqsCommand::GetSeqsCommand(string option) { if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; } } + //initialize outputTypes + vector tempOutNames; + outputTypes["fasta"] = tempOutNames; + outputTypes["taxonomy"] = tempOutNames; + outputTypes["name"] = tempOutNames; + outputTypes["group"] = tempOutNames; + outputTypes["alignreport"] = tempOutNames; + outputTypes["list"] = tempOutNames; + outputTypes["qfile"] = tempOutNames; + //if the user changes the output directory command factory will send this info to us in the output parameter outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; } @@ -99,6 +162,14 @@ GetSeqsCommand::GetSeqsCommand(string option) { //if the user has not given a path then, add inputdir. else leave path alone. if (path == "") { parameters["taxonomy"] = inputDir + it->second; } } + + it = parameters.find("qfile"); + //user has given a template file + if(it != parameters.end()){ + path = m->hasPath(it->second); + //if the user has not given a path then, add inputdir. else leave path alone. + if (path == "") { parameters["qfile"] = inputDir + it->second; } + } } @@ -131,11 +202,15 @@ GetSeqsCommand::GetSeqsCommand(string option) { if (taxfile == "not open") { abort = true; } else if (taxfile == "not found") { taxfile = ""; } + qualfile = validParameter.validFile(parameters, "qfile", true); + if (qualfile == "not open") { abort = true; } + else if (qualfile == "not found") { qualfile = ""; } + string usedDups = "true"; string temp = validParameter.validFile(parameters, "dups", false); if (temp == "not found") { temp = "false"; usedDups = ""; } dups = m->isTrue(temp); - if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "")) { m->mothurOut("You must provide one of the following: fasta, name, group, alignreport, taxonomy or listfile."); m->mothurOutEndLine(); abort = true; } + if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "") && (qualfile == "")) { m->mothurOut("You must provide one of the following: fasta, name, group, alignreport, taxonomy, quality or listfile."); m->mothurOutEndLine(); abort = true; } if ((usedDups != "") && (namefile == "")) { m->mothurOut("You may only use dups with the name option."); m->mothurOutEndLine(); abort = true; } @@ -151,9 +226,9 @@ GetSeqsCommand::GetSeqsCommand(string option) { void GetSeqsCommand::help(){ try { - m->mothurOut("The get.seqs command reads an .accnos file and any of the following file types: fasta, name, group, list, taxonomy or alignreport file.\n"); + m->mothurOut("The get.seqs command reads an .accnos file and any of the following file types: fasta, name, group, list, taxonomy, quality or alignreport file.\n"); m->mothurOut("It outputs a file containing only the sequences in the .accnos file.\n"); - m->mothurOut("The get.seqs command parameters are accnos, fasta, name, group, list, taxonomy, alignreport and dups. You must provide accnos and at least one of the other parameters.\n"); + m->mothurOut("The get.seqs command parameters are accnos, fasta, name, group, list, taxonomy, qfile, alignreport and dups. You must provide accnos and at least one of the other parameters.\n"); m->mothurOut("The dups parameter allows you to add the entire line from a name file if you add any name from the line. default=false. \n"); m->mothurOut("The get.seqs command should be in the following format: get.seqs(accnos=yourAccnos, fasta=yourFasta).\n"); m->mothurOut("Example get.seqs(accnos=amazon.accnos, fasta=amazon.fasta).\n"); @@ -184,8 +259,11 @@ int GetSeqsCommand::execute(){ if (alignfile != "") { readAlign(); } if (listfile != "") { readList(); } if (taxfile != "") { readTax(); } + if (qualfile != "") { readQual(); } - if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; } + if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; } + + m->mothurOut("Selected " + toString(names.size()) + " sequences."); m->mothurOutEndLine(); if (outputNames.size() != 0) { m->mothurOutEndLine(); @@ -206,8 +284,9 @@ int GetSeqsCommand::execute(){ //********************************************************************************************************************** int GetSeqsCommand::readFasta(){ try { - if (outputDir == "") { outputDir += m->hasPath(fastafile); } - string outputFileName = outputDir + m->getRootName(m->getSimpleName(fastafile)) + "pick" + m->getExtension(fastafile); + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(fastafile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)) + "pick" + m->getExtension(fastafile); ofstream out; m->openOutputFile(outputFileName, out); @@ -238,8 +317,9 @@ int GetSeqsCommand::readFasta(){ in.close(); out.close(); + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } - outputNames.push_back(outputFileName); + outputNames.push_back(outputFileName); outputTypes["fasta"].push_back(outputFileName); return 0; @@ -250,10 +330,76 @@ int GetSeqsCommand::readFasta(){ } } //********************************************************************************************************************** +int GetSeqsCommand::readQual(){ + try { + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(qualfile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(qualfile)) + "pick" + m->getExtension(qualfile); + ofstream out; + m->openOutputFile(outputFileName, out); + + + ifstream in; + m->openInputFile(qualfile, in); + string name; + + bool wroteSomething = false; + + + while(!in.eof()){ + string saveName = ""; + string name = ""; + string scores = ""; + + in >> name; + + if (name.length() != 0) { + saveName = name.substr(1); + while (!in.eof()) { + char c = in.get(); + if (c == 10 || c == 13){ break; } + else { name += c; } + } + m->gobble(in); + } + + while(in){ + char letter= in.get(); + if(letter == '>'){ in.putback(letter); break; } + else{ scores += letter; } + } + + m->gobble(in); + + if (names.count(saveName) != 0) { + wroteSomething = true; + + out << name << endl << scores; + } + + m->gobble(in); + } + in.close(); + out.close(); + + + if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } + outputNames.push_back(outputFileName); outputTypes["qfile"].push_back(outputFileName); + + return 0; + + } + catch(exception& e) { + m->errorOut(e, "GetSeqsCommand", "readQual"); + exit(1); + } +} +//********************************************************************************************************************** int GetSeqsCommand::readList(){ try { - if (outputDir == "") { outputDir += m->hasPath(listfile); } - string outputFileName = outputDir + m->getRootName(m->getSimpleName(listfile)) + "pick" + m->getExtension(listfile); + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(listfile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(listfile)) + "pick" + m->getExtension(listfile); ofstream out; m->openOutputFile(outputFileName, out); @@ -310,7 +456,7 @@ int GetSeqsCommand::readList(){ out.close(); if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } - outputNames.push_back(outputFileName); + outputNames.push_back(outputFileName); outputTypes["list"].push_back(outputFileName); return 0; @@ -323,8 +469,9 @@ int GetSeqsCommand::readList(){ //********************************************************************************************************************** int GetSeqsCommand::readName(){ try { - if (outputDir == "") { outputDir += m->hasPath(namefile); } - string outputFileName = outputDir + m->getRootName(m->getSimpleName(namefile)) + "pick" + m->getExtension(namefile); + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(namefile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(namefile)) + "pick" + m->getExtension(namefile); ofstream out; m->openOutputFile(outputFileName, out); @@ -402,7 +549,7 @@ int GetSeqsCommand::readName(){ out.close(); if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } - outputNames.push_back(outputFileName); + outputNames.push_back(outputFileName); outputTypes["name"].push_back(outputFileName); return 0; @@ -416,8 +563,9 @@ int GetSeqsCommand::readName(){ //********************************************************************************************************************** int GetSeqsCommand::readGroup(){ try { - if (outputDir == "") { outputDir += m->hasPath(groupfile); } - string outputFileName = outputDir + m->getRootName(m->getSimpleName(groupfile)) + "pick" + m->getExtension(groupfile); + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(groupfile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(groupfile)) + "pick" + m->getExtension(groupfile); ofstream out; m->openOutputFile(outputFileName, out); @@ -449,7 +597,7 @@ int GetSeqsCommand::readGroup(){ out.close(); if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } - outputNames.push_back(outputFileName); + outputNames.push_back(outputFileName); outputTypes["group"].push_back(outputFileName); return 0; @@ -462,8 +610,9 @@ int GetSeqsCommand::readGroup(){ //********************************************************************************************************************** int GetSeqsCommand::readTax(){ try { - if (outputDir == "") { outputDir += m->hasPath(taxfile); } - string outputFileName = outputDir + m->getRootName(m->getSimpleName(taxfile)) + "pick" + m->getExtension(taxfile); + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(taxfile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(taxfile)) + "pick" + m->getExtension(taxfile); ofstream out; m->openOutputFile(outputFileName, out); @@ -493,7 +642,7 @@ int GetSeqsCommand::readTax(){ out.close(); if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } - outputNames.push_back(outputFileName); + outputNames.push_back(outputFileName); outputTypes["taxonomy"].push_back(outputFileName); return 0; @@ -507,8 +656,9 @@ int GetSeqsCommand::readTax(){ //alignreport file has a column header line then all other lines contain 16 columns. we just want the first column since that contains the name int GetSeqsCommand::readAlign(){ try { - if (outputDir == "") { outputDir += m->hasPath(alignfile); } - string outputFileName = outputDir + m->getRootName(m->getSimpleName(alignfile)) + "pick.align.report"; + string thisOutputDir = outputDir; + if (outputDir == "") { thisOutputDir += m->hasPath(alignfile); } + string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(alignfile)) + "pick.align.report"; ofstream out; m->openOutputFile(outputFileName, out); @@ -560,7 +710,7 @@ int GetSeqsCommand::readAlign(){ out.close(); if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine(); } - outputNames.push_back(outputFileName); + outputNames.push_back(outputFileName); outputTypes["alignreport"].push_back(outputFileName); return 0;