X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=filterseqscommand.cpp;h=a677eef9729ef5f1035db03f0a5daff9f7280e36;hb=92de7f976371d41441ad41f02ca83af8b43cef5c;hp=a12058e4785d00e1c21b84ea453ce82c4f62797d;hpb=f663afa231c9bc1b5e18e0ea3bdd2b2ee784f5b2;p=mothur.git diff --git a/filterseqscommand.cpp b/filterseqscommand.cpp index a12058e..a677eef 100644 --- a/filterseqscommand.cpp +++ b/filterseqscommand.cpp @@ -10,20 +10,77 @@ #include "filterseqscommand.h" #include "sequence.hpp" -/**************************************************************************************/ +//********************************************************************************************************************** +vector FilterSeqsCommand::setParameters(){ + try { + CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pfasta); + CommandParameter phard("hard", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(phard); + CommandParameter ptrump("trump", "String", "", "*", "", "", "",false,false); parameters.push_back(ptrump); + CommandParameter psoft("soft", "Number", "", "0", "", "", "",false,false); parameters.push_back(psoft); + CommandParameter pvertical("vertical", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(pvertical); + CommandParameter pprocessors("processors", "Number", "", "1", "", "", "",false,false); parameters.push_back(pprocessors); + CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir); + CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir); + + vector myArray; + for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); } + return myArray; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "setParameters"); + exit(1); + } +} +//********************************************************************************************************************** +string FilterSeqsCommand::getHelpString(){ + try { + string helpString = ""; + helpString += "The filter.seqs command reads a file containing sequences and creates a .filter and .filter.fasta file.\n"; + helpString += "The filter.seqs command parameters are fasta, trump, soft, hard, processors and vertical. \n"; + helpString += "The fasta parameter is required, unless you have a valid current fasta file. You may enter several fasta files to build the filter from and filter, by separating their names with -'s.\n"; + helpString += "For example: fasta=abrecovery.fasta-amazon.fasta \n"; + helpString += "The trump option will remove a column if the trump character is found at that position in any sequence of the alignment. Default=*, meaning no trump. \n"; + helpString += "A soft mask removes any column where the dominant base (i.e. A, T, G, C, or U) does not occur in at least a designated percentage of sequences. Default=0.\n"; + helpString += "The hard parameter allows you to enter a file containing the filter you want to use.\n"; + helpString += "The vertical parameter removes columns where all sequences contain a gap character. The default is T.\n"; + helpString += "The processors parameter allows you to specify the number of processors to use. The default is 1.\n"; + helpString += "The filter.seqs command should be in the following format: \n"; + helpString += "filter.seqs(fasta=yourFastaFile, trump=yourTrump) \n"; + helpString += "Example filter.seqs(fasta=abrecovery.fasta, trump=.).\n"; + helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n"; + return helpString; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "getHelpString"); + exit(1); + } +} +//********************************************************************************************************************** +FilterSeqsCommand::FilterSeqsCommand(){ + try { + abort = true; calledHelp = true; + setParameters(); + vector tempOutNames; + outputTypes["fasta"] = tempOutNames; + outputTypes["filter"] = tempOutNames; + } + catch(exception& e) { + m->errorOut(e, "FilterSeqsCommand", "FilterSeqsCommand"); + exit(1); + } +} +/**************************************************************************************/ FilterSeqsCommand::FilterSeqsCommand(string option) { try { - abort = false; + abort = false; calledHelp = false; filterFileName = ""; //allow user to run help - if(option == "help") { help(); abort = true; } + if(option == "help") { help(); abort = true; calledHelp = true; } else { - //valid paramters for this command - string Array[] = {"fasta", "trump", "soft", "hard", "vertical", "outputdir","inputdir", "processors"}; - vector myArray (Array, Array+(sizeof(Array)/sizeof(string))); + vector myArray = setParameters(); OptionParser parser(option); map parameters = parser.getParameters(); @@ -36,6 +93,11 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; } } + //initialize outputTypes + vector tempOutNames; + outputTypes["fasta"] = tempOutNames; + outputTypes["filter"] = tempOutNames; + //if the user changes the input directory command factory will send this info to us in the output parameter string inputDir = validParameter.validFile(parameters, "inputdir", false); if (inputDir == "not found"){ inputDir = ""; } @@ -60,7 +122,11 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { //check for required parameters fasta = validParameter.validFile(parameters, "fasta", false); - if (fasta == "not found") { m->mothurOut("fasta is a required parameter for the filter.seqs command."); m->mothurOutEndLine(); abort = true; } + if (fasta == "not found") { + fasta = m->getFastaFile(); + if (fasta != "") { fastafileNames.push_back(fasta); m->mothurOut("Using " + fasta + " as input file for the fasta parameter."); m->mothurOutEndLine(); } + else { m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; } + } else { m->splitAtDash(fasta, fastafileNames); @@ -80,10 +146,25 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { if (m->getDefaultPath() != "") { //default path is set string tryPath = m->getDefaultPath() + m->getSimpleName(fastafileNames[i]); m->mothurOut("Unable to open " + fastafileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine(); - ableToOpen = m->openInputFile(tryPath, in, "noerror"); + ifstream in2; + ableToOpen = m->openInputFile(tryPath, in2, "noerror"); + in2.close(); fastafileNames[i] = tryPath; } } + + //if you can't open it, try default location + if (ableToOpen == 1) { + if (m->getOutputDir() != "") { //default path is set + string tryPath = m->getOutputDir() + m->getSimpleName(fastafileNames[i]); + m->mothurOut("Unable to open " + fastafileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine(); + ifstream in2; + ableToOpen = m->openInputFile(tryPath, in2, "noerror"); + in2.close(); + fastafileNames[i] = tryPath; + } + } + in.close(); if (ableToOpen == 1) { @@ -122,7 +203,8 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { temp = validParameter.validFile(parameters, "soft", false); if (temp == "not found") { soft = 0; } else { soft = (float)atoi(temp.c_str()) / 100.0; } - temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found"){ temp = "1"; } + temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found"){ temp = m->getProcessors(); } + m->setProcessors(temp); convert(temp, processors); vertical = validParameter.validFile(parameters, "vertical", false); @@ -140,39 +222,12 @@ FilterSeqsCommand::FilterSeqsCommand(string option) { exit(1); } } - -//********************************************************************************************************************** - -void FilterSeqsCommand::help(){ - try { - - m->mothurOut("The filter.seqs command reads a file containing sequences and creates a .filter and .filter.fasta file.\n"); - m->mothurOut("The filter.seqs command parameters are fasta, trump, soft, hard, processors and vertical. \n"); - m->mothurOut("The fasta parameter is required. You may enter several fasta files to build the filter from and filter, by separating their names with -'s.\n"); - m->mothurOut("For example: fasta=abrecovery.fasta-amazon.fasta \n"); - m->mothurOut("The trump parameter .... The default is ...\n"); - m->mothurOut("The soft parameter .... The default is ....\n"); - m->mothurOut("The hard parameter allows you to enter a file containing the filter you want to use.\n"); - m->mothurOut("The vertical parameter removes columns where all sequences contain a gap character. The default is T.\n"); - m->mothurOut("The processors parameter allows you to specify the number of processors to use. The default is 1.\n"); - m->mothurOut("The filter.seqs command should be in the following format: \n"); - m->mothurOut("filter.seqs(fasta=yourFastaFile, trump=yourTrump) \n"); - m->mothurOut("Example filter.seqs(fasta=abrecovery.fasta, trump=.).\n"); - m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n"); - - } - catch(exception& e) { - m->errorOut(e, "FilterSeqsCommand", "help"); - exit(1); - } -} - /**************************************************************************************/ int FilterSeqsCommand::execute() { try { - if (abort == true) { return 0; } + if (abort == true) { if (calledHelp) { return 0; } return 2; } ifstream inFASTA; m->openInputFile(fastafileNames[0], inFASTA); @@ -188,7 +243,7 @@ int FilterSeqsCommand::execute() { m->mothurOutEndLine(); m->mothurOutEndLine(); - if (m->control_pressed) { return 0; } + if (m->control_pressed) { outputTypes.clear(); return 0; } #ifdef USE_MPI int pid; @@ -207,7 +262,7 @@ int FilterSeqsCommand::execute() { m->openOutputFile(filterFile, outFilter); outFilter << filter << endl; outFilter.close(); - outputNames.push_back(filterFile); + outputNames.push_back(filterFile); outputTypes["filter"].push_back(filterFile); #ifdef USE_MPI } @@ -226,7 +281,7 @@ int FilterSeqsCommand::execute() { if(filter[i] == '1'){ filteredLength++; } } - if (m->control_pressed) { for(int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; } + if (m->control_pressed) { outputTypes.clear(); for(int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; } m->mothurOutEndLine(); @@ -235,6 +290,12 @@ int FilterSeqsCommand::execute() { m->mothurOut("Length of the original alignment: " + toString(alignmentLength)); m->mothurOutEndLine(); m->mothurOut("Number of sequences used to construct filter: " + toString(numSeqs)); m->mothurOutEndLine(); + //set fasta file as new current fastafile + string current = ""; + itTypes = outputTypes.find("fasta"); + if (itTypes != outputTypes.end()) { + if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); } + } m->mothurOutEndLine(); m->mothurOut("Output File Names: "); m->mothurOutEndLine(); @@ -261,7 +322,7 @@ int FilterSeqsCommand::filterSequences() { string filteredFasta = outputDir + m->getRootName(m->getSimpleName(fastafileNames[s])) + "filter.fasta"; #ifdef USE_MPI - int pid, start, end, numSeqsPerProcessor, num; + int pid, numSeqsPerProcessor, num; int tag = 2001; vectorMPIPos; @@ -270,7 +331,6 @@ int FilterSeqsCommand::filterSequences() { MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are MPI_File outMPI; - MPI_File tempMPI; MPI_File inMPI; int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY; int inMode=MPI_MODE_RDONLY; @@ -373,7 +433,7 @@ int FilterSeqsCommand::filterSequences() { if (m->control_pressed) { return 1; } #endif #endif - outputNames.push_back(filteredFasta); + outputNames.push_back(filteredFasta); outputTypes["fasta"].push_back(filteredFasta); } return 0; @@ -542,7 +602,11 @@ int FilterSeqsCommand::createProcessesRunFilter(string F, string filename) { out.close(); exit(0); - }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); } + }else { + m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); + for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); } + exit(0); + } } //force parent to wait until all the processes are done @@ -906,7 +970,11 @@ int FilterSeqsCommand::createProcessesCreateFilter(Filters& F, string filename) out.close(); exit(0); - }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); } + }else { + m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); + for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); } + exit(0); + } } //parent do your part