X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=extractRef.cpp;h=0a46de0950534ea4c22f3acb8f239de66ef8415f;hb=1b2999c4407ef8419fb89b66b843b7141caff4da;hp=c4f24a9a079b37a25d39d6fa969ff9ac1482f3de;hpb=a95154919f950f86de9104b2b9dcf1f0c7e83387;p=rsem.git

diff --git a/extractRef.cpp b/extractRef.cpp
index c4f24a9..0a46de0 100644
--- a/extractRef.cpp
+++ b/extractRef.cpp
@@ -86,8 +86,14 @@ bool buildTranscript(int sp, int ep) {
 		int start = items[i].getStart();
 		int end = items[i].getEnd();
 
-		assert(strand == items[i].getStrand());
-		assert(seqname == items[i].getSeqName());
+		if (strand != items[i].getStrand()) {
+		  fprintf(stderr, "According to the GTF file given, a transcript has exons from different orientations!\n");
+		  exit(-1);
+		}
+		if (seqname != items[i].getSeqName()) {
+		  fprintf(stderr, "According to the GTF file given, a transcript has exons on multiple chromosomes!\n");
+		  exit(-1);
+		}
 
 		if (cur_e + 1 < start) {
 			if (cur_s > 0) vec.push_back(Interval(cur_s, cur_e));
@@ -113,6 +119,7 @@ void parse_gtf_file(char* gtfF) {
 
 	items.clear();
  	while (getline(fin, line)) {
+ 		if (line[0] == '#') continue; // if this line is comment, jump it
  		item.parse(line);
  		string feature = item.getFeature();
  		if (feature == "exon") {
@@ -127,14 +134,14 @@ void parse_gtf_file(char* gtfF) {
  			else {
  		 		if (hasMappingFile) {
  		 			tid = item.getTranscriptID();
- 		 		    mi_iter = mi_table.find(tid);
- 		 		    if (mi_iter == mi_table.end()) {
- 		 		    	fprintf(stderr, "Mapping Info is not correct, cannot find %s's gene_id!\n", tid.c_str());
- 		 		    	exit(-1);
- 		 		    }
- 		 		    //assert(iter != table.end());
- 		 		    gid = mi_iter->second;
- 		 		    item.setGeneID(gid);
+					mi_iter = mi_table.find(tid);
+					if (mi_iter == mi_table.end()) {
+					  fprintf(stderr, "Mapping Info is not correct, cannot find %s's gene_id!\n", tid.c_str());
+					  exit(-1);
+					}
+					//assert(iter != table.end());
+					gid = mi_iter->second;
+					item.setGeneID(gid);
  		 		}
  				items.push_back(item);
  			}
@@ -240,7 +247,7 @@ void writeResults(char* refName) {
 }
 
 int main(int argc, char* argv[]) {
-	if (argc < 6 || (hasMappingFile = atoi(argv[4])) && argc < 7) {
+  if (argc < 6 || ((hasMappingFile = atoi(argv[4])) && argc < 7)) {
 		printf("Usage: rsem-extract-reference-transcripts refName quiet gtfF hasMappingFile [mappingFile] chromosome_file_1 [chromosome_file_2 ...]\n");
 		exit(-1);
 	}
@@ -296,7 +303,9 @@ int main(int argc, char* argv[]) {
 
 	for (int i = 1; i <= M; i++) {
 		if (seqs[i] == "") {
-			fprintf(stderr, "%s's sequence is empty! You must provide all chromosome files of transcripts which are presented in the .gtf file!\n", transcripts.getTranscriptAt(i).getTranscriptID().c_str());
+			const Transcript& transcript = transcripts.getTranscriptAt(i);
+			fprintf(stderr, "Cannot extract transcript %s's sequence from chromosome %s, whose information might not be provided! Please check if the chromosome directory is set correctly or the list of chromosome files is complete.\n", \
+					transcript.getTranscriptID().c_str(), transcript.getGeneID().c_str());
 			exit(-1);
 		}
 	}