X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=code_ruby%2Flib%2Fmaasha%2Fseq.rb;h=4a0af47ed0f5fde9d5ffe87ca19a3caec087f6d6;hb=b982cc677363d7458963b610ec53bf2c4f7476a9;hp=6c0f35b358e67135b35c4032b7fc9e9a78e6e0fb;hpb=98f798c64a0b795f642bceaa06fb9b951a519ead;p=biopieces.git

diff --git a/code_ruby/lib/maasha/seq.rb b/code_ruby/lib/maasha/seq.rb
index 6c0f35b..4a0af47 100644
--- a/code_ruby/lib/maasha/seq.rb
+++ b/code_ruby/lib/maasha/seq.rb
@@ -67,15 +67,16 @@ TRANS_TAB11 = {
   "GTG" => "V", "GCG" => "A", "GAG" => "E", "GGG" => "G"
 }
 
-# Quality scores bases
-SCORE_BASE = 64
-SCORE_MIN  = 0
-SCORE_MAX  = 40
 
 # Error class for all exceptions to do with Seq.
 class SeqError < StandardError; end
 
 class Seq
+  # Quality scores bases
+  SCORE_BASE = 64
+  SCORE_MIN  = 0
+  SCORE_MAX  = 40
+
   include Digest
   include Trim
 
@@ -149,6 +150,7 @@ class Seq
   # by inspecting the first 100 residues.
   def type_guess!
     self.type = self.type_guess
+    self
   end
 
   # Returns the length of a sequence.
@@ -333,17 +335,13 @@ class Seq
     key
   end
 
-  # Method to reverse complement sequence.
-  def reverse_complement
-    self.reverse
-    self.complement
-    self
+  # Method to reverse the sequence.
+  def reverse
+    Seq.new(self.seq_name, self.seq.reverse, self.type, self.qual ? self.qual.reverse : self.qual)
   end
 
-  alias :revcomp :reverse_complement
-
   # Method to reverse the sequence.
-  def reverse
+  def reverse!
     self.seq.reverse!
     self.qual.reverse! if self.qual
     self
@@ -353,6 +351,26 @@ class Seq
   def complement
     raise SeqError, "Cannot complement 0 length sequence" if self.length == 0
 
+    entry          = Seq.new
+    entry.seq_name = self.seq_name
+    entry.type     = self.type
+    entry.qual     = self.qual
+
+    if self.is_dna?
+      entry.seq = self.seq.tr('AGCUTRYWSMKHDVBNagcutrywsmkhdvbn', 'TCGAAYRWSKMDHBVNtcgaayrwskmdhbvn')
+    elsif self.is_rna?
+      entry.seq = self.seq.tr('AGCUTRYWSMKHDVBNagcutrywsmkhdvbn', 'UCGAAYRWSKMDHBVNucgaayrwskmdhbvn')
+    else
+      raise SeqError, "Cannot complement sequence type: #{self.type}"
+    end
+
+    entry
+  end
+
+  # Method that complements sequence including ambiguity codes.
+  def complement!
+    raise SeqError, "Cannot complement 0 length sequence" if self.length == 0
+
     if self.is_dna?
       self.seq.tr!('AGCUTRYWSMKHDVBNagcutrywsmkhdvbn', 'TCGAAYRWSKMDHBVNtcgaayrwskmdhbvn')
     elsif self.is_rna?
@@ -360,6 +378,8 @@ class Seq
     else
       raise SeqError, "Cannot complement sequence type: #{self.type}"
     end
+
+    self
   end
 
   # Method to determine the Hamming Distance between
@@ -389,7 +409,12 @@ class Seq
     seq_new
   end
 
-  # Method to shuffle a sequence readomly inline.
+  # Method to return a new Seq object with shuffled sequence.
+  def shuffle
+    Seq.new(self.seq_name, self.seq.split('').shuffle!.join, self.type, self.qual)
+  end
+
+  # Method to shuffle a sequence randomly inline.
   def shuffle!
     self.seq = self.seq.split('').shuffle!.join
     self
@@ -482,21 +507,6 @@ class Seq
     ((self.seq.scan(/[a-z]/).size.to_f / (self.len - self.indels).to_f) * 100).round(2)
   end
 
-  # Hard masks sequence residues where the corresponding quality score
-  # is below a given cutoff.
-  def mask_seq_hard_old(cutoff)
-    seq    = self.seq.upcase
-    scores = self.qual
-    i      = 0
-
-    scores.each_char do |score|
-      seq[i] = 'N' if score.ord - SCORE_BASE < cutoff
-      i += 1 
-    end
-
-    self.seq = seq
-  end
-
   # Hard masks sequence residues where the corresponding quality score
   # is below a given cutoff.
   def mask_seq_hard!(cutoff)
@@ -538,13 +548,30 @@ class Seq
   # Method that determines if a quality score string can be
   # absolutely identified as base 33.
   def qual_base33?
-    self.qual.match(/[!-:]/)
+    self.qual.match(/[!-:]/) ? true : false
   end
 
   # Method that determines if a quality score string can be
   # absolutely identified as base 64.
   def qual_base64?
-    self.qual.match(/[K-h]/)
+    self.qual.match(/[K-h]/) ? true : false
+  end
+
+  # Method to determine if a quality score is valid.
+  def qual_valid?(encoding)
+    raise SeqError, "Missing qual" if self.qual.nil?
+
+    case encoding.downcase
+    when "sanger"     then return true if self.qual.match(/^[!-~]*$/)
+    when "454"        then return true if self.qual.match(/^[@-~]*$/)
+    when "solexa"     then return true if self.qual.match(/^[;-~]*$/)
+    when "illumina13" then return true if self.qual.match(/^[@-~]*$/)
+    when "illumina15" then return true if self.qual.match(/^[@-~]*$/)
+    when "illumina18" then return true if self.qual.match(/^[!-~]*$/)
+    else raise SeqError, "unknown quality score encoding: #{encoding}"
+    end
+
+    false
   end
 
   # Method to convert quality scores inbetween formats.
@@ -552,7 +579,7 @@ class Seq
   # 454        base 64, range  0-40 
   # Solexa     base 64, range -5-40 
   # Illumina13 base 64, range  0-40 
-  # Illumina15 base 64, range  3-40 
+  # Illumina15 base 64, range  0-40 
   # Illumina18 base 33, range  0-41 
   def convert_scores!(from, to)
     unless from == to
@@ -575,7 +602,7 @@ class Seq
       when "illumina13" then na_qual += 64
       when "illumina15" then na_qual += 64
       when "illumina18" then na_qual += 33
-      else raise SeqError, "unknown quality score encoding: #{from}"
+      else raise SeqError, "unknown quality score encoding: #{to}"
       end
 
       self.qual = na_qual.to_s