X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=ccode.h;h=456b735df524ff3eed9c2688be95f8537c0d0c86;hb=bd27c2b0612942815b7417c79f7ee41f669a2a34;hp=cced0b7d404b4145a437b677f37fa869bb8abb3f;hpb=6d7408400b6bbdde4173922c5dca528f9f4e0a22;p=mothur.git diff --git a/ccode.h b/ccode.h index cced0b7..456b735 100644 --- a/ccode.h +++ b/ccode.h @@ -14,6 +14,7 @@ #include "dist.h" #include "decalc.h" +/***********************************************************/ //This class was created using the algorythms described in the // "Evaluating putative chimeric sequences from PCR-amplified products" paper //by Juan M. Gonzalez, Johannes Zimmerman and Cesareo Saiz-Jimenez. @@ -23,46 +24,66 @@ class Ccode : public Chimera { public: - Ccode(string, string); + Ccode(string, string, bool, string, int, int, string); //fasta, template, filter, mask, window, numWanted, outputDir ~Ccode(); - void getChimeras(); - void print(ostream&); - - void setCons(string c) {} - void setQuantiles(string q) {} - + int getChimeras(Sequence* query); + Sequence print(ostream&, ostream&); + #ifdef USE_MPI + Sequence print(MPI_File&, MPI_File&); + #endif private: Dist* distCalc; DeCalculator* decalc; - int iters; - string fastafile, templateFile; + int iters, window, numWanted; + string fastafile, mapInfo; + Sequence* querySeq; - vector lines; - vector templateLines; - vector querySeqs; - vector templateSeqs; + map spotMap; + map::iterator it; - vector windows; - vector< vector > closest; //closest[0] is a vector of sequence at are closest to queryseqs[0]... - vector< vector > averageRef; //averageRef[0] is the average distance at each window for the references for querySeqs[0] - vector< vector > averageQuery; //averageQuery[0] is the average distance at each winow for the query for querySeqs[0] + vector windows; //windows is the vector of window breaks for query + int windowSizes; //windowSizes is the size of the windows for query + map trim; //trim is the map containing the starting and ending positions for query + vector closest; //closest is a vector of sequence at are closest to query + vector averageRef; //averageRef is the average distance at each window for the references for query + vector averageQuery; //averageQuery is the average distance at each winow for the query for query + vector sumRef; //sumRef is the sum of distances at each window for the references for query + vector sumSquaredRef; //sumSquaredRef is the sum of squared distances at each window for the references for query + vector sumQuery; //sumQuery is the sum of distances at each window for the comparison of query to references for query + vector sumSquaredQuery; //sumSquaredQuery is the sum of squared distances at each window for the comparison of query to references for query + vector varRef; //varRef is the variance among references seqs at each window for query + vector varQuery; //varQuery is the variance among references and query at each window + vector sdRef; //sdRef is the standard deviation of references seqs at each window for query + vector sdQuery; //sdQuery is the standard deviation of references and query at each window + vector anova; //anova is the vector of anova scores for each window for query + int refCombo; //refCombo is the number of reference sequences combinations for query + vector isChimericConfidence; //isChimericConfidence indicates whether query is chimeric at a given window according to the confidence limits + vector isChimericTStudent; //isChimericConfidence indicates whether query is chimeric at a given window according to the confidence limits + vector isChimericANOVA; //isChimericConfidence indicates whether query is chimeric at a given window according to the confidence limits - vector< vector > findClosest(int, int, int); - void removeBadReferenceSeqs(vector&, int); //removes sequences from closest that are to different of too similar to eachother. - void trimSequences(); + vector findClosest(Sequence*, int); + void removeBadReferenceSeqs(vector&); //removes sequences from closest that are to different of too similar to eachother. + void trimSequences(Sequence*); vector findWindows(); - vector getAverageRef(vector); - vector getAverageQuery (vector, int); - + void getAverageRef(vector); //fills sumRef, averageRef, sumSquaredRef and refCombo. + void getAverageQuery (vector, Sequence*); //fills sumQuery, averageQuery, sumSquaredQuery. + void findVarianceRef (); //fills varRef and sdRef also sets minimum error rate to 0.001 to avoid divide by 0. + void findVarianceQuery (); //fills varQuery and sdQuery + void determineChimeras (); //fills anova, isChimericConfidence, isChimericTStudent and isChimericANOVA. int getDiff(string, string); //return number of mismatched bases, a gap to base is not counted as a mismatch + float getT(int); + float getF(int); - void createProcessesClosest(); - + #ifdef USE_MPI + int printMapping(string&); + MPI_File outMap; + #endif + }; /***********************************************************/