X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bp_bin%2Fwrite_fastq;h=d15c96f9dd31eb0ac6792b6b70d092ddcdd29bb6;hb=2f0fd91b461033529a4a72e161bd133252a22eb6;hp=f6050f38555071932cab4ad91eaba821c8d84a83;hpb=2dddbfc4c5563421b83cce0e81f2e559ed982bab;p=biopieces.git diff --git a/bp_bin/write_fastq b/bp_bin/write_fastq index f6050f3..d15c96f 100755 --- a/bp_bin/write_fastq +++ b/bp_bin/write_fastq @@ -31,41 +31,36 @@ require 'maasha/biopieces' require 'maasha/fastq' -allowed_enc = 'sanger,solexa,illumina1.3,illumina1.5,illumina1.8' +allowed_enc = 'base_33,base_64' casts = [] -casts << {:long=>'no_stream', :short=>'x', :type=>'flag', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} -casts << {:long=>'data_out', :short=>'o', :type=>'file', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} -casts << {:long=>'encoding', :short=>'e', :type=>'string', :mandatory=>false, :default=>'illumina1.3', :allowed=>allowed_enc, :disallowed=>nil} -casts << {:long=>'compress', :short=>'Z', :type=>'flag', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} +casts << {long: 'no_stream', short: 'x', type: 'flag', mandatory: false, default: nil, allowed: nil, disallowed: nil} +casts << {long: 'data_out', short: 'o', type: 'file', mandatory: false, default: nil, allowed: nil, disallowed: nil} +casts << {long: 'encoding', short: 'e', type: 'string', mandatory: false, default: 'base_33', allowed: allowed_enc, disallowed: nil} +casts << {long: 'compress', short: 'Z', type: 'string', mandatory: false, default: nil, allowed: "gzip,bzip,bzip2", disallowed: nil} options = Biopieces.options_parse(ARGV, casts) -encoding = options[:encoding].downcase.delete('.') +encoding = options[:encoding].to_sym +compress = options[:compress] ? options[:compress].to_sym : nil + +raise "--data_out is mandatory for compressed output" if compress and not options[:data_out] Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| - if options[:data_out] - if options[:compress] - io_out = Zlib::GzipWriter.open(options[:data_out]) - else - io_out = Fastq.open(options[:data_out], 'w') - end - else - io_out = $stdout - end + fastq_out = options[:data_out] ? Fastq.open(options[:data_out], 'w', compress: compress) : STDOUT input.each do |record| if record[:SEQ_NAME] and record[:SEQ] and record[:SCORES] entry = Seq.new_bp(record) - entry.convert_scores!('illumina13', encoding) + entry.qual_convert!(:base_33, encoding) - io_out.puts entry.to_fastq + fastq_out.puts entry.to_fastq end - output.puts record unless options.has_key? :no_stream + output.puts record unless options[:no_stream] end - io_out.close + fastq_out.close end