X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bp_bin%2Fwrite_blast;h=b3f6c72180565265e34713903c184bf37e72c325;hb=5de6112b70b59420b245ce636a8b2e3c90acbe00;hp=4cd1d4413a253b7338666f848168a0f9929fba03;hpb=e1e61f6b2e3f51a98c0be95573d6955dc921a697;p=biopieces.git diff --git a/bp_bin/write_blast b/bp_bin/write_blast index 4cd1d44..b3f6c72 100755 --- a/bp_bin/write_blast +++ b/bp_bin/write_blast @@ -1,6 +1,143 @@ #!/usr/bin/env perl +# Copyright (C) 2007-2009 Martin A. Hansen. + +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. + +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. + +# You should have received a copy of the GNU General Public License +# along with this program; if not, write to the Free Software +# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. + +# http://www.gnu.org/copyleft/gpl.html + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# Write tabular BLAST output from the stream. + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + use warnings; use strict; - +use Maasha::Fasta; use Maasha::Biopieces; + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +my ( $options, $in, $out, $record, $first, $entry, $data_out ); + +$options = Maasha::Biopieces::parse_options( + [ + { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + { long => 'data_out', short => 'o', type => 'file', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + { long => 'comment', short => 'c', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + { long => 'compress', short => 'Z', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + ] +); + +$in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); +$out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); +$data_out = Maasha::Biopieces::write_stream( $options->{ "data_out" }, $options->{ "compress" } ) ; + +$first = 1; + +while ( $record = Maasha::Biopieces::get_record( $in ) ) +{ + if ( $entry = biopiece2blast_tab( $record ) ) + { + if ( $options->{ "comment" } and $first ) + { + print $data_out "# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score\n"; + + $first = 0; + } + + print $data_out join( "\t", @{ $entry } ), "\n"; + } + + Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" }; +} + +close $data_out; + +Maasha::Biopieces::close_stream( $in ); +Maasha::Biopieces::close_stream( $out ); + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> SUBROUTINES <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +sub biopiece2blast_tab +{ + # Martin A. Hansen, May 2009. + + # Convert a Biopiece record to a BLAST tabular entry. + # Note that we correct positions because BLAST is 1-based. + + my ( $record, # hashref + ) = @_; + + # Returns a list. + + my ( @fields, $s_beg, $s_end ); + + return undef if not defined $record->{ 'REC_TYPE' } or not $record->{ 'REC_TYPE' } eq "BLAST"; + + if ( $record->{ "STRAND" } eq '+' ) + { + $s_beg = $record->{ "S_BEG" }; + $s_end = $record->{ "S_END" }; + } + else + { + $s_beg = $record->{ "S_END" }; + $s_end = $record->{ "S_BEG" }; + } + + $fields[ 0 ] = $record->{ "Q_ID" }; + $fields[ 1 ] = $record->{ "S_ID" }; + $fields[ 2 ] = $record->{ "IDENT" }; + $fields[ 3 ] = $record->{ "ALIGN_LEN" }; + $fields[ 4 ] = $record->{ "MISMATCHES" }; + $fields[ 5 ] = $record->{ "GAPS" }; + $fields[ 6 ] = $record->{ "Q_BEG" } + 1; + $fields[ 7 ] = $record->{ "Q_END" } + 1; + $fields[ 8 ] = $s_beg + 1; + $fields[ 9 ] = $s_end + 1; + $fields[ 10 ] = $record->{ "E_VAL" }; + $fields[ 11 ] = $record->{ "BIT_SCORE" }; + + return wantarray ? @fields : \@fields; +} + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +BEGIN +{ + Maasha::Biopieces::status_set(); +} + + +END +{ + Maasha::Biopieces::status_log(); +} + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +__END__