X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bp_bin%2Fpcr_seq;h=8700276e16287559ed9fe37f1d33b8c26c382e7a;hb=5ced0e7864e9645e56c6c618da1f04e0a4d667fc;hp=f54577373e75f7f32f24752c3a54ff31eaedafa8;hpb=e95fec9ae319e4da65e7dd5700abf7b4a036b411;p=biopieces.git

diff --git a/bp_bin/pcr_seq b/bp_bin/pcr_seq
index f545773..8700276 100755
--- a/bp_bin/pcr_seq
+++ b/bp_bin/pcr_seq
@@ -51,6 +51,7 @@ class Pcr
 
       command =  "scan_for_matches"
       # command << " -c"
+      command << " -o 1"
       command << " #{pat_file}"
       command << " < #{@infile}"
       command << " > #{outfile}"
@@ -152,14 +153,25 @@ class Pattern
 end
 
 casts = []
-casts << {:long=>'forward',  :short=>'f', :type=>'string', :mandatory=>true, :default=>nil,  :allowed=>nil, :disallowed=>nil}
-casts << {:long=>'reverse',  :short=>'r', :type=>'string', :mandatory=>true, :default=>nil,  :allowed=>nil, :disallowed=>nil}
-casts << {:long=>'max_dist', :short=>'m', :type=>'uint',   :mandatory=>true, :default=>5000, :allowed=>nil, :disallowed=>"0"}
+casts << {:long=>'forward',    :short=>'f', :type=>'string', :mandatory=>false, :default=>nil,  :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'forward_rc', :short=>'F', :type=>'string', :mandatory=>false, :default=>nil,  :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'reverse',    :short=>'r', :type=>'string', :mandatory=>false, :default=>nil,  :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'reverse_rc', :short=>'R', :type=>'string', :mandatory=>false, :default=>nil,  :allowed=>nil, :disallowed=>nil}
+casts << {:long=>'max_dist',   :short=>'m', :type=>'uint',   :mandatory=>true, :default=>5000, :allowed=>nil, :disallowed=>"0"}
 
 options = Biopieces.options_parse(ARGV, casts)
 tmpdir  = Biopieces.mktmpdir
 infile  = File.join(tmpdir, "in.fna")
 
+if options[:forward_rc]
+  options[:forward] = Seq.new("test", options[:forward_rc], 'dna').revcomp.seq
+end
+
+if options[:reverse_rc]
+  options[:reverse] = Seq.new("test", options[:reverse_rc], 'dna').revcomp.seq
+end
+
+raise ArgumentError, "no adaptor specified" unless options[:forward] or options[:reverse]
 Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output|
   Fasta.open(infile, mode="w") do |ios|
     input.each_record do |record|