X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bp_bin%2Fcalc_fixedstep;h=e98b63a9c8828337f093310573e59a08e7164c86;hb=5de6112b70b59420b245ce636a8b2e3c90acbe00;hp=fdf5bd287f78613d2aea83bb9b15f855cad57e77;hpb=c32b13419ca8e524a5c98869048d0be670ee76d0;p=biopieces.git diff --git a/bp_bin/calc_fixedstep b/bp_bin/calc_fixedstep index fdf5bd2..e98b63a 100755 --- a/bp_bin/calc_fixedstep +++ b/bp_bin/calc_fixedstep @@ -1,6 +1,120 @@ #!/usr/bin/env perl +# Copyright (C) 2007-2010 Martin A. Hansen. + +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. + +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. + +# You should have received a copy of the GNU General Public License +# along with this program; if not, write to the Free Software +# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. + +# http://www.gnu.org/copyleft/gpl.html + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# Calculate fixedstep entries from records in the stream. + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + use warnings; use strict; +use Maasha::Biopieces; +use Maasha::Filesys; +use Maasha::UCSC::BED; +use Maasha::UCSC::Wiggle; + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +my ( $options, $in, $out, $record, $tmp_dir, $bed_file, $fh_in, $fh_out, + $file_hash, $chr, $bed_entry, $fixedstep_file, $fixedstep_entry ); + +$options = Maasha::Biopieces::parse_options( + [ + { long => 'check', short => 'C', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + { long => 'log10', short => 'l', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef }, + ] +); + +$in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); +$out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); + +$tmp_dir = Maasha::Biopieces::get_tmpdir(); + +$bed_file = "$tmp_dir/calc_fixedstep.bed"; +$fh_out = Maasha::Filesys::file_write_open( $bed_file ); + +while ( $record = Maasha::Biopieces::get_record( $in ) ) +{ + if ( $bed_entry = Maasha::UCSC::BED::biopiece2bed( $record ) ) { + Maasha::UCSC::BED::bed_entry_put( $bed_entry, $fh_out, undef, $options->{ 'check' } ); + } + + Maasha::Biopieces::put_record( $record, $out ); +} + +close $fh_out; + +$file_hash = Maasha::UCSC::BED::bed_file_split_on_chr( $bed_file, $tmp_dir ); + +unlink $bed_file; + +foreach $chr ( sort keys %{ $file_hash } ) +{ + $bed_file = $file_hash->{ $chr }; + + $fixedstep_file = Maasha::UCSC::Wiggle::fixedstep_calc( $bed_file, $chr, $options->{ 'log10' } ); + + #$fixedstep_file = "$bed_file.fixedstep"; + + # Maasha::Common::run( "bed2fixedstep", "< $bed_file > $fixedstep_file" ); + + $fh_in = Maasha::Filesys::file_read_open( $fixedstep_file ); + + while ( $fixedstep_entry = Maasha::UCSC::Wiggle::fixedstep_entry_get( $fh_in ) ) + { + if ( $record = Maasha::UCSC::Wiggle::fixedstep2biopiece( $fixedstep_entry ) ) { + Maasha::Biopieces::put_record( $record, $out ); + } + } + + close $fh_in; + + unlink $bed_file; + unlink $fixedstep_file; +} + +Maasha::Biopieces::close_stream( $in ); +Maasha::Biopieces::close_stream( $out ); + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +BEGIN +{ + Maasha::Biopieces::status_set(); +} + + +END +{ + Maasha::Biopieces::status_log(); +} + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + -use Maasha::BioRun; +__END__