X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bp_bin%2Fcalc_bit_scores;h=6da3afbddde48793a9ccd3ef43d5130fbb25f58b;hb=af282a65d141826c15944437b07a0353dd14e79c;hp=7105a3a49afd127b66c971246469eb57ef1acf77;hpb=f7e5c1b54c562cc1b25fe419cacd4da9ac01f129;p=biopieces.git diff --git a/bp_bin/calc_bit_scores b/bp_bin/calc_bit_scores deleted file mode 120000 index 7105a3a..0000000 --- a/bp_bin/calc_bit_scores +++ /dev/null @@ -1 +0,0 @@ -../code_perl/Maasha/bin/calc_bit_scores \ No newline at end of file diff --git a/bp_bin/calc_bit_scores b/bp_bin/calc_bit_scores new file mode 100755 index 0000000..6da3afb --- /dev/null +++ b/bp_bin/calc_bit_scores @@ -0,0 +1,111 @@ +#!/usr/bin/env perl + +# Copyright (C) 2007-2009 Martin A. Hansen. + +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. + +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. + +# You should have received a copy of the GNU General Public License +# along with this program; if not, write to the Free Software +# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. + +# http://www.gnu.org/copyleft/gpl.html + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + +# Calculate the bit score for each position based on an alignment in the stream. + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +use warnings; +use strict; +use Maasha::Biopieces; +use Maasha::Seq; + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +my ( $options, $in, $out, $record, $count, $i, $res, $type, $bit_max, %freq_hash, $bit_height, $bit_diff ); + +$options = Maasha::Biopieces::parse_options(); + +$in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } ); +$out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } ); + +$count = 0; + +while ( $record = Maasha::Biopieces::get_record( $in ) ) +{ + if ( $record->{ "SEQ" } ) + { + $type = Maasha::Seq::seq_guess_type( $record->{ "SEQ" } ) if not $type; + + for ( $i = 0; $i < length $record->{ "SEQ" }; $i++ ) + { + $res = substr $record->{ "SEQ" }, $i, 1; + + next if $res =~ /-|_|~|\./; + + $freq_hash{ $i }{ $res }++; + } + + $count++; + } + else + { + Maasha::Biopieces::put_record( $record, $out ); + } +} + +undef $record; + +if ( $type eq "PROTEIN" ) { + $bit_max = 4; +} else { + $bit_max = 2; +} + +for ( $i = 0; $i < keys %freq_hash; $i++ ) +{ + $bit_height = Maasha::Seq::seqlogo_calc_bit_height( $freq_hash{ $i }, $count ); + + $bit_diff = $bit_max - $bit_height; + + $record->{ "V" . ( $i ) } = sprintf( "%.2f", $bit_diff ); +} + +Maasha::Biopieces::put_record( $record, $out ); + +Maasha::Biopieces::close_stream( $in ); +Maasha::Biopieces::close_stream( $out ); + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +BEGIN +{ + Maasha::Biopieces::status_set(); +} + + +END +{ + Maasha::Biopieces::status_log(); +} + + +# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< + + +__END__