X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bp_bin%2Fbowtie_seq;h=07374216593d0a6f24d7fd858f6de3b21c6377ec;hb=5de6112b70b59420b245ce636a8b2e3c90acbe00;hp=f7229f106fba9abe71105bd6adca0758237c9848;hpb=15fc1cc28abfb41e8630c731fd6459dcdcd08e10;p=biopieces.git

diff --git a/bp_bin/bowtie_seq b/bp_bin/bowtie_seq
index f7229f1..0737421 100755
--- a/bp_bin/bowtie_seq
+++ b/bp_bin/bowtie_seq
@@ -21,19 +21,25 @@
 
 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
 
-# Use bowtie to map sequences in the stream against a specified genome.
+# Use bowtie to map sequences in the stream against a specified genome or index.
 
 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
 
 
 use warnings;
 use strict;
+use Data::Dumper;
 use Maasha::Biopieces;
 use Maasha::Common;
 use Maasha::Fastq;
 use Maasha::Fasta;
 use Maasha::Calc;
 
+use constant {
+    SEQ_NAME => 0,
+    SEQ      => 1,
+    SCORES   => 2,
+};
 
 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
 
@@ -51,7 +57,7 @@ $options = Maasha::Biopieces::parse_options(
     ]   
 );
 
-Maasha::Common::error( qq(both --index_hame and --genome specified) ) if     $options->{ "genome" } and     $options->{ "index_name" };
+Maasha::Common::error( qq(both --index_name and --genome specified) ) if     $options->{ "genome" } and     $options->{ "index_name" };
 Maasha::Common::error( qq(no --index_name or --genome specified) )    if not $options->{ "genome" } and not $options->{ "index_name" };
 
 $in  = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
@@ -74,6 +80,7 @@ while ( $record = Maasha::Biopieces::get_record( $in ) )
     if ( $entry = Maasha::Fastq::biopiece2fastq( $record ) )
     {
         Maasha::Common::error( "Mixed FASTA and FASTQ entries in stream" ) if defined $type and $type ne "FASTQ";
+        Maasha::Common::error( "Sequence longer than 1024 not allowed")    if length( $entry->[ SEQ ] ) > 1024;
         Maasha::Fastq::put_entry( $entry, $fh_out );
 
         $type = "FASTQ";
@@ -81,6 +88,7 @@ while ( $record = Maasha::Biopieces::get_record( $in ) )
     elsif ( $entry = Maasha::Fasta::biopiece2fasta( $record ) )
     {
         Maasha::Common::error( "Mixed FASTA and FASTQ entries in stream" ) if defined $type and $type ne "FASTA";
+        Maasha::Common::error( "Sequence longer than 1024 not allowed")    if length( $entry->[ SEQ ] ) > 1024;
         Maasha::Fasta::put_entry( $entry, $fh_out );
 
         $type = "FASTA";
@@ -92,7 +100,10 @@ while ( $record = Maasha::Biopieces::get_record( $in ) )
 close $fh_out;
 
 push @args, "-n $options->{ 'mismatches' }";
+push @args, "-v $options->{ 'mismatches' }";  # DANGER: using seed mismatches as alignment mismatches - may work, may not!
 push @args, "-f" if $type eq "FASTA";
+push @args, "-p $options->{ 'cpus' }";
+push @args, "--phred33-quals" unless $type eq "FASTA";
 
 if ( defined $options->{ 'max_hits' } ) {
     push @args, "-k $options->{ 'max_hits' }";
@@ -121,7 +132,6 @@ while ( $line = <$fh_in> )
     chomp $line;
 
     @fields = split /\t/, $line;
-
     $record = bowtie2biopiece( \@fields );
 
     Maasha::Biopieces::put_record( $record, $out );
@@ -149,21 +159,22 @@ sub bowtie2biopiece
 
     # Returns a hash.
 
-    my ( $record, @scores );
-
-    $record->{ 'Q_ID' }     = $entry->[ 0 ];
-    $record->{ 'STRAND' }   = $entry->[ 1 ];
-    $record->{ 'S_ID' }     = $entry->[ 2 ];
-    $record->{ 'S_BEG' }    = $entry->[ 3 ];
-    $record->{ 'SEQ' }      = $entry->[ 4 ];
-    $record->{ 'SCORES' }   = $entry->[ 5 ];
-    $record->{ 'MISMATCH' } = $entry->[ 6 ];
-
+    my ( $record, $s_id, $s_len, $hits );
+
+    $record->{ 'Q_ID' }       = $entry->[ 0 ];
+    $record->{ 'STRAND' }     = $entry->[ 1 ];
+    $record->{ 'S_ID' }       = $entry->[ 2 ];
+    $record->{ 'S_BEG' }      = $entry->[ 3 ];
+    $record->{ 'SEQ' }        = $entry->[ 4 ];
+    $record->{ 'SCORES' }     = $entry->[ 5 ];
+    $record->{ 'SCORE' }      = $entry->[ 6 ] + 1;
+    $record->{ 'ALIGN' }      = $entry->[ 7 ] || '.';
+    $record->{ 'S_LEN' }      = length $entry->[ 4 ];
     $record->{ 'SEQ_LEN' }    = length $entry->[ 4 ];
     $record->{ 'S_END' }      = $record->{ 'S_BEG' } + $record->{ 'SEQ_LEN' } - 1;
-    $record->{ 'SCORES' }     =~ s/(.)/ord( $1 ) - 33 . ";"/ge; # http://maq.sourceforge.net/fastq.shtml
-    $record->{ 'SCORE_MEAN' } = sprintf( "%.2f", Maasha::Calc::mean( [ split /;/, $record->{ 'SCORES' } ] ) );
+    $record->{ 'SCORES' }     =~ s/(.)/chr( ( ord( $1 ) - 33 ) + 64 )/ge; # convert phred scores to illumina scores
 
+    $record->{ 'HITS' }       = '.';
     $record->{ 'REC_TYPE' } = "BOWTIE";
 
     return wantarray ? %{ $record } : $record;