X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bam_import.c;h=427140db9cbe9e3c80d173b0d69e31b1774c3037;hb=d0e30eec1158752010659982342a611fc91ae8e3;hp=6697a9ffb4e89e4d67e00e8a5917c1c71ea3b405;hpb=42e756837f7acb91bfd1134ef16d423e71c913d7;p=samtools.git

diff --git a/bam_import.c b/bam_import.c
index 6697a9f..427140d 100644
--- a/bam_import.c
+++ b/bam_import.c
@@ -5,7 +5,12 @@
 #include <stdlib.h>
 #include <unistd.h>
 #include <assert.h>
+#ifdef _WIN32
+#include <fcntl.h>
+#endif
+#include "kstring.h"
 #include "bam.h"
+#include "sam_header.h"
 #include "kseq.h"
 #include "khash.h"
 
@@ -35,6 +40,25 @@ unsigned char bam_nt16_table[256] = {
 	15,15,15,15, 15,15,15,15, 15,15,15,15, 15,15,15,15
 };
 
+unsigned short bam_char2flag_table[256] = {
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,BAM_FREAD1,BAM_FREAD2,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	BAM_FPROPER_PAIR,0,BAM_FMREVERSE,0, 0,BAM_FMUNMAP,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, BAM_FDUP,0,BAM_FQCFAIL,0, 0,0,0,0, 0,0,0,0,
+	BAM_FPAIRED,0,BAM_FREVERSE,BAM_FSECONDARY, 0,BAM_FUNMAP,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0,
+	0,0,0,0, 0,0,0,0, 0,0,0,0, 0,0,0,0
+};
+
 char *bam_nt16_rev_table = "=ACMGRSVTWYHKDBN";
 
 struct __tamFile_t {
@@ -42,6 +66,7 @@ struct __tamFile_t {
 	kstream_t *ks;
 	kstring_t *str;
 	uint64_t n_lines;
+	int is_first;
 };
 
 char **__bam_get_lines(const char *fn, int *_n) // for bam_plcmd.c only
@@ -97,12 +122,13 @@ bam_header_t *sam_header_read2(const char *fn)
 	kstring_t *str;
 	kh_ref_t *hash;
 	khiter_t k;
-	hash = kh_init(ref);
+	if (fn == 0) return 0;
 	fp = (strcmp(fn, "-") == 0)? gzdopen(fileno(stdin), "r") : gzopen(fn, "r");
-	assert(fp);
+	if (fp == 0) return 0;
+	hash = kh_init(ref);
 	ks = ks_init(fp);
 	str = (kstring_t*)calloc(1, sizeof(kstring_t));
-	while (ks_getuntil(ks, 0, str, &dret) >= 0) {
+	while (ks_getuntil(ks, 0, str, &dret) > 0) {
 		char *s = strdup(str->s);
 		int len, i;
 		i = kh_size(hash);
@@ -144,14 +170,46 @@ static inline void append_text(bam_header_t *header, kstring_t *str)
 	header->l_text += str->l + 1;
 	header->text[header->l_text] = 0;
 }
-int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
+
+int sam_header_parse_rg(bam_header_t *h)
 {
-	int ret, doff, doff0, dret;
-	bam1_core_t *c = &b->core;
-	kstring_t *str = fp->str;
-	kstream_t *ks = fp->ks;
+	if (h->dict == 0) h->dict = sam_header_parse2(h->text);
+	if (h->rg2lib) h->rg2lib = sam_header2tbl(h->dict, "RG", "ID", "LB");
+	return sam_tbl_size(h->rg2lib);
+}
 
-	while ((ret = ks_getuntil(fp->ks, 0, str, &dret)) >= 0 && str->s[0] == '@') { // skip header
+int sam_header_parse(bam_header_t *h)
+{
+	void *tbl;
+	char **tmp;
+	int i;
+	free(h->target_len); free(h->target_name);
+	h->n_targets = 0; h->target_len = 0; h->target_name = 0;
+	if (h->l_text < 3) return 0;
+	if (h->dict == 0) h->dict = sam_header_parse2(h->text);
+	tbl = sam_header2tbl(h->dict, "SQ", "SN", "LN");
+	h->n_targets = sam_tbl_size(tbl);
+	if (h->n_targets == 0) {
+		sam_tbl_destroy(tbl);
+		return 0;
+	}
+	h->target_len = (uint32_t*)calloc(h->n_targets, 4);
+	h->target_name = (char**)calloc(h->n_targets, sizeof(void*));
+	tmp = (char**)calloc(h->n_targets, sizeof(void*));
+	sam_tbl_pair(tbl, h->target_name, tmp);
+	for (i = 0; i < h->n_targets; ++i)
+		h->target_len[i] = atoi(tmp[i]);
+	free(tmp);
+	sam_tbl_destroy(tbl);
+	return h->n_targets;
+}
+
+bam_header_t *sam_header_read(tamFile fp)
+{
+	int ret, dret;
+	bam_header_t *header = bam_header_init();
+	kstring_t *str = fp->str;
+	while ((ret = ks_getuntil(fp->ks, KS_SEP_TAB, str, &dret)) >= 0 && str->s[0] == '@') { // skip header
 		str->s[str->l] = dret; // note that str->s is NOT null terminated!!
 		append_text(header, str);
 		if (dret != '\n') {
@@ -161,7 +219,28 @@ int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
 		}
 		++fp->n_lines;
 	}
-	while (ret == 0) ret = ks_getuntil(fp->ks, 0, str, &dret); // special consideration for "\r\n"
+	sam_header_parse(header);
+	bam_init_header_hash(header);
+	fp->is_first = 1;
+	return header;
+}
+
+int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
+{
+	int ret, doff, doff0, dret, z = 0;
+	bam1_core_t *c = &b->core;
+	kstring_t *str = fp->str;
+	kstream_t *ks = fp->ks;
+
+	if (fp->is_first) {
+		fp->is_first = 0;
+		ret = str->l;
+	} else {
+		do { // special consideration for empty lines
+			ret = ks_getuntil(fp->ks, KS_SEP_TAB, str, &dret);
+			if (ret >= 0) z += str->l + 1;
+		} while (ret == 0);
+	}
 	if (ret < 0) return -1;
 	++fp->n_lines;
 	doff = 0;
@@ -171,11 +250,28 @@ int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
 		memcpy(alloc_data(b, doff + c->l_qname) + doff, str->s, c->l_qname);
 		doff += c->l_qname;
 	}
-	{ // flag, tid, pos, qual
-		ret = ks_getuntil(ks, 0, str, &dret); c->flag = atoi(str->s);
-		ret = ks_getuntil(ks, 0, str, &dret); c->tid = bam_get_tid(header, str->s);
-		ret = ks_getuntil(ks, 0, str, &dret); c->pos = isdigit(str->s[0])? atoi(str->s) - 1 : -1;
-		ret = ks_getuntil(ks, 0, str, &dret); c->qual = isdigit(str->s[0])? atoi(str->s) : 0;
+	{ // flag
+		long flag;
+		char *s;
+		ret = ks_getuntil(ks, KS_SEP_TAB, str, &dret); z += str->l + 1;
+		flag = strtol((char*)str->s, &s, 0);
+		if (*s) { // not the end of the string
+			flag = 0;
+			for (s = str->s; *s; ++s)
+				flag |= bam_char2flag_table[(int)*s];
+		}
+		c->flag = flag;
+	}
+	{ // tid, pos, qual
+		ret = ks_getuntil(ks, KS_SEP_TAB, str, &dret); z += str->l + 1; c->tid = bam_get_tid(header, str->s);
+		if (c->tid < 0 && strcmp(str->s, "*")) {
+			if (header->n_targets == 0) {
+				fprintf(stderr, "[sam_read1] missing header? Abort!\n");
+				exit(1);
+			} else fprintf(stderr, "[sam_read1] reference '%s' is recognized as '*'.\n", str->s);
+		}
+		ret = ks_getuntil(ks, KS_SEP_TAB, str, &dret); z += str->l + 1; c->pos = isdigit(str->s[0])? atoi(str->s) - 1 : -1;
+		ret = ks_getuntil(ks, KS_SEP_TAB, str, &dret); z += str->l + 1; c->qual = isdigit(str->s[0])? atoi(str->s) : 0;
 		if (ret < 0) return -2;
 	}
 	{ // cigar
@@ -183,7 +279,8 @@ int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
 		int i, op;
 		long x;
 		c->n_cigar = 0;
-		if (ks_getuntil(ks, 0, str, &dret) < 0) return -3;
+		if (ks_getuntil(ks, KS_SEP_TAB, str, &dret) < 0) return -3;
+		z += str->l + 1;
 		if (str->s[0] != '*') {
 			for (s = str->s; *s; ++s) {
 				if (isalpha(*s)) ++c->n_cigar;
@@ -193,7 +290,7 @@ int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
 			for (i = 0, s = str->s; i != c->n_cigar; ++i) {
 				x = strtol(s, &t, 10);
 				op = toupper(*t);
-				if (op == 'M') op = BAM_CMATCH;
+				if (op == 'M' || op == '=' || op == 'X') op = BAM_CMATCH;
 				else if (op == 'I') op = BAM_CINS;
 				else if (op == 'D') op = BAM_CDEL;
 				else if (op == 'N') op = BAM_CREF_SKIP;
@@ -207,36 +304,51 @@ int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
 			if (*s) parse_error(fp->n_lines, "unmatched CIGAR operation");
 			c->bin = bam_reg2bin(c->pos, bam_calend(c, bam1_cigar(b)));
 			doff += c->n_cigar * 4;
-		} else c->bin = bam_reg2bin(c->pos, c->pos + 1);
+		} else {
+			if (!(c->flag&BAM_FUNMAP)) {
+				fprintf(stderr, "Parse warning at line %lld: mapped sequence without CIGAR\n", (long long)fp->n_lines);
+				c->flag |= BAM_FUNMAP;
+			}
+			c->bin = bam_reg2bin(c->pos, c->pos + 1);
+		}
 	}
 	{ // mtid, mpos, isize
-		ret = ks_getuntil(ks, 0, str, &dret); c->mtid = strcmp(str->s, "=")? bam_get_tid(header, str->s) : c->tid;
-		ret = ks_getuntil(ks, 0, str, &dret); c->mpos = isdigit(str->s[0])? atoi(str->s) - 1 : -1;
-		ret = ks_getuntil(ks, 0, str, &dret); c->isize = (str->s[0] == '-' || isdigit(str->s[0]))? atoi(str->s) : 0;
+		ret = ks_getuntil(ks, KS_SEP_TAB, str, &dret); z += str->l + 1;
+		c->mtid = strcmp(str->s, "=")? bam_get_tid(header, str->s) : c->tid;
+		ret = ks_getuntil(ks, KS_SEP_TAB, str, &dret); z += str->l + 1;
+		c->mpos = isdigit(str->s[0])? atoi(str->s) - 1 : -1;
+		ret = ks_getuntil(ks, KS_SEP_TAB, str, &dret); z += str->l + 1;
+		c->isize = (str->s[0] == '-' || isdigit(str->s[0]))? atoi(str->s) : 0;
 		if (ret < 0) return -4;
 	}
 	{ // seq and qual
 		int i;
-		uint8_t *p;
-		if (ks_getuntil(ks, 0, str, &dret) < 0) return -5; // seq
-		c->l_qseq = strlen(str->s);
-		if (c->n_cigar && c->l_qseq != (int32_t)bam_cigar2qlen(c, bam1_cigar(b)))
-			parse_error(fp->n_lines, "CIGAR and sequence length are inconsistent");
-		p = (uint8_t*)alloc_data(b, doff + c->l_qseq + (c->l_qseq+1)/2) + doff;
-		bzero(p, (c->l_qseq+1)/2);
-		for (i = 0; i < c->l_qseq; ++i)
-			p[i/2] |= bam_nt16_table[(int)str->s[i]] << 4*(1-i%2);
-		if (ks_getuntil(ks, 0, str, &dret) < 0) return -6; // qual
-		if (c->l_qseq != strlen(str->s))
+		uint8_t *p = 0;
+		if (ks_getuntil(ks, KS_SEP_TAB, str, &dret) < 0) return -5; // seq
+		z += str->l + 1;
+		if (strcmp(str->s, "*")) {
+			c->l_qseq = strlen(str->s);
+			if (c->n_cigar && c->l_qseq != (int32_t)bam_cigar2qlen(c, bam1_cigar(b)))
+				parse_error(fp->n_lines, "CIGAR and sequence length are inconsistent");
+			p = (uint8_t*)alloc_data(b, doff + c->l_qseq + (c->l_qseq+1)/2) + doff;
+			memset(p, 0, (c->l_qseq+1)/2);
+			for (i = 0; i < c->l_qseq; ++i)
+				p[i/2] |= bam_nt16_table[(int)str->s[i]] << 4*(1-i%2);
+		} else c->l_qseq = 0;
+		if (ks_getuntil(ks, KS_SEP_TAB, str, &dret) < 0) return -6; // qual
+		z += str->l + 1;
+		if (strcmp(str->s, "*") && c->l_qseq != strlen(str->s))
 			parse_error(fp->n_lines, "sequence and quality are inconsistent");
 		p += (c->l_qseq+1)/2;
-		for (i = 0; i < c->l_qseq; ++i) p[i] = str->s[i] - 33;
+		if (strcmp(str->s, "*") == 0) for (i = 0; i < c->l_qseq; ++i) p[i] = 0xff;
+		else for (i = 0; i < c->l_qseq; ++i) p[i] = str->s[i] - 33;
 		doff += c->l_qseq + (c->l_qseq+1)/2;
 	}
 	doff0 = doff;
 	if (dret != '\n' && dret != '\r') { // aux
-		while (ks_getuntil(ks, 0, str, &dret) >= 0) {
+		while (ks_getuntil(ks, KS_SEP_TAB, str, &dret) >= 0) {
 			uint8_t *s, type, key[2];
+			z += str->l + 1;
 			if (str->l < 6 || str->s[2] != ':' || str->s[4] != ':')
 				parse_error(fp->n_lines, "missing colon in auxiliary data");
 			key[0] = str->s[0]; key[1] = str->s[1];
@@ -285,6 +397,11 @@ int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
 				*s++ = 'f';
 				*(float*)s = (float)atof(str->s + 5);
 				s += 4; doff += 5;
+			} else if (type == 'd') {
+				s = alloc_data(b, doff + 9) + doff;
+				*s++ = 'd';
+				*(float*)s = (float)atof(str->s + 9);
+				s += 8; doff += 9;
 			} else if (type == 'Z' || type == 'H') {
 				int size = 1 + (str->l - 5) + 1;
 				if (type == 'H') { // check whether the hex string is valid
@@ -307,17 +424,18 @@ int sam_read1(tamFile fp, bam_header_t *header, bam1_t *b)
 	}
 	b->l_aux = doff - doff0;
 	b->data_len = doff;
-	return 0;
+	return z;
 }
 
 tamFile sam_open(const char *fn)
 {
 	tamFile fp;
+	gzFile gzfp = (strcmp(fn, "-") == 0)? gzdopen(fileno(stdin), "rb") : gzopen(fn, "rb");
+	if (gzfp == 0) return 0;
 	fp = (tamFile)calloc(1, sizeof(struct __tamFile_t));
 	fp->str = (kstring_t*)calloc(1, sizeof(kstring_t));
-	fp->fp = (strcmp(fn, "-") == 0)? gzdopen(fileno(stdin), "r") : gzopen(fn, "r");
+	fp->fp = gzfp;
 	fp->ks = ks_init(fp->fp);
-	fp->n_lines = 0;
 	return fp;
 }
 
@@ -330,41 +448,3 @@ void sam_close(tamFile fp)
 		free(fp);
 	}
 }
-
-static void taf2baf_core(const char *fntaf, const char *fnbaf, bam_header_t *header)
-{
-	bamFile fpbaf;
-	bam1_t *b;
-	tamFile fp;
-	int ret;
-
-	b = (bam1_t*)calloc(1, sizeof(bam1_t));
-	fpbaf = (strcmp(fnbaf, "-") == 0)? bam_dopen(fileno(stdout), "w") : bam_open(fnbaf, "w");
-	fp = sam_open(fntaf);
-	ret = sam_read1(fp, header, b);
-	bam_header_write(fpbaf, header);
-	if (ret >= 0) {
-		bam_write1(fpbaf, b);
-		while (sam_read1(fp, header, b) >= 0) bam_write1(fpbaf, b);
-	}
-	bam_close(fpbaf);
-	free(b->data); free(b);
-	sam_close(fp);
-}
-
-int bam_taf2baf(int argc, char *argv[])
-{
-	int c;
-	bam_header_t *header;
-
-	while ((c = getopt(argc, argv, "")) >= 0) {
-	}
-	if (optind + 3 > argc) {
-		fprintf(stderr, "Usage: bamtk import <in.ref_list> <in.sam> <out.bam>\n");
-		return 1;
-	}
-	header = sam_header_read2(argv[optind]);
-	taf2baf_core(argv[optind+1], argv[optind+2], header);
-	bam_header_destroy(header);
-	return 0;
-}