X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bam.h;h=11f8028cf6d96ece1390074c6fb155437ce5f766;hb=2594fb7e8055fefc08b37a14ad437aa9bafbe837;hp=246b020d679a07f073cfbae8dc40a8d57b501d25;hpb=3384f6c6c1642ada4edae9204ca1202672de7d5a;p=samtools.git diff --git a/bam.h b/bam.h index 246b020..11f8028 100644 --- a/bam.h +++ b/bam.h @@ -40,7 +40,7 @@ @copyright Genome Research Ltd. */ -#define BAM_VERSION "0.1.17 (r973:277)" +#define BAM_VERSION "0.1.18-dev (r982:313)" #include #include @@ -89,7 +89,7 @@ typedef struct { char **target_name; uint32_t *target_len; void *dict, *hash, *rg2lib; - size_t l_text, n_text; + uint32_t l_text, n_text; char *text; } bam_header_t; @@ -134,20 +134,35 @@ typedef struct { /* CIGAR operations. */ -/*! @abstract CIGAR: match */ +/*! @abstract CIGAR: M = match or mismatch*/ #define BAM_CMATCH 0 -/*! @abstract CIGAR: insertion to the reference */ +/*! @abstract CIGAR: I = insertion to the reference */ #define BAM_CINS 1 -/*! @abstract CIGAR: deletion from the reference */ +/*! @abstract CIGAR: D = deletion from the reference */ #define BAM_CDEL 2 -/*! @abstract CIGAR: skip on the reference (e.g. spliced alignment) */ +/*! @abstract CIGAR: N = skip on the reference (e.g. spliced alignment) */ #define BAM_CREF_SKIP 3 -/*! @abstract CIGAR: clip on the read with clipped sequence present in qseq */ +/*! @abstract CIGAR: S = clip on the read with clipped sequence + present in qseq */ #define BAM_CSOFT_CLIP 4 -/*! @abstract CIGAR: clip on the read with clipped sequence trimmed off */ +/*! @abstract CIGAR: H = clip on the read with clipped sequence trimmed off */ #define BAM_CHARD_CLIP 5 -/*! @abstract CIGAR: padding */ +/*! @abstract CIGAR: P = padding */ #define BAM_CPAD 6 +/*! @abstract CIGAR: equals = match */ +#define BAM_CEQUAL 7 +/*! @abstract CIGAR: X = mismatch */ +#define BAM_CDIFF 8 +#define BAM_CBACK 9 + +#define BAM_CIGAR_STR "MIDNSHP=XB" +#define BAM_CIGAR_TYPE 0x3C1A7 + +#define bam_cigar_op(c) ((c)&BAM_CIGAR_MASK) +#define bam_cigar_oplen(c) ((c)>>BAM_CIGAR_SHIFT) +#define bam_cigar_opchr(c) (BAM_CIGAR_STR[bam_cigar_op(c)]) +#define bam_cigar_gen(l, o) ((l)<>((o)<<1)&3) // bit 1: consume query; bit 2: consume reference /*! @typedef @abstract Structure for core alignment information. @@ -240,7 +255,10 @@ typedef struct __bam_iter_t *bam_iter_t; @param i The i-th position, 0-based @return 4-bit integer representing the base. */ -#define bam1_seqi(s, i) ((s)[(i)/2] >> 4*(1-(i)%2) & 0xf) +//#define bam1_seqi(s, i) ((s)[(i)/2] >> 4*(1-(i)%2) & 0xf) +#define bam1_seqi(s, i) ((s)[(i)>>1] >> ((~(i)&1)<<2) & 0xf) + +#define bam1_seq_seti(s, i, c) ( (s)[(i)>>1] = ((s)[(i)>>1] & 0xf<<(((i)&1)<<2)) | (c)<<((~(i)&1)<<2) ) /*! @function @abstract Get query sequence and quality @@ -270,6 +288,8 @@ extern int bam_is_be; */ extern int bam_verbose; +extern int bam_no_B; + /*! @abstract Table for converting a nucleotide character to the 4-bit encoding. */ extern unsigned char bam_nt16_table[256]; @@ -415,6 +435,8 @@ extern "C" { */ int bam_read1(bamFile fp, bam1_t *b); + int bam_remove_B(bam1_t *b); + /*! @abstract Write an alignment to BAM. @param fp BAM file handler