X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=bam.c;h=8b517565026f276d860f91634789ec8fa2f36195;hb=d0e30eec1158752010659982342a611fc91ae8e3;hp=2edb2a8b1e339fb99ee3a9c085d1d61bd1881db2;hpb=6f6733c7551a2f4697c46acb4a516fa04bba3e40;p=samtools.git diff --git a/bam.c b/bam.c index 2edb2a8..8b51756 100644 --- a/bam.c +++ b/bam.c @@ -1,9 +1,11 @@ #include #include +#include #include #include "bam.h" #include "bam_endian.h" #include "kstring.h" +#include "sam_header.h" int bam_is_be = 0; char *bam_flag2char_table = "pPuUrR12sfd\0\0\0\0\0"; @@ -12,28 +14,6 @@ char *bam_flag2char_table = "pPuUrR12sfd\0\0\0\0\0"; * CIGAR related routines * **************************/ -int bam_segreg(int32_t pos, const bam1_core_t *c, const uint32_t *cigar, bam_segreg_t *reg) -{ - unsigned k; - int32_t x = c->pos, y = 0; - int state = 0; - for (k = 0; k < c->n_cigar; ++k) { - int op = cigar[k] & BAM_CIGAR_MASK; // operation - int l = cigar[k] >> BAM_CIGAR_SHIFT; // length - if (state == 0 && (op == BAM_CMATCH || op == BAM_CDEL || op == BAM_CINS) && x + l > pos) { - reg->tbeg = x; reg->qbeg = y; reg->cbeg = k; - state = 1; - } - if (op == BAM_CMATCH) { x += l; y += l; } - else if (op == BAM_CDEL || op == BAM_CREF_SKIP) x += l; - else if (op == BAM_CINS || op == BAM_CSOFT_CLIP) y += l; - if (state == 1 && (op == BAM_CSOFT_CLIP || op == BAM_CHARD_CLIP || op == BAM_CREF_SKIP || k == c->n_cigar - 1)) { - reg->tend = x; reg->qend = y; reg->cend = k; - } - } - return state? 0 : -1; -} - uint32_t bam_calend(const bam1_core_t *c, const uint32_t *cigar) { uint32_t k, end; @@ -80,10 +60,9 @@ void bam_header_destroy(bam_header_t *header) free(header->target_len); } free(header->text); -#ifndef BAM_NO_HASH - if (header->rg2lib) bam_strmap_destroy(header->rg2lib); + if (header->dict) sam_header_free(header->dict); + if (header->rg2lib) sam_tbl_destroy(header->rg2lib); bam_destroy_header_hash(header); -#endif free(header); } @@ -91,7 +70,15 @@ bam_header_t *bam_header_read(bamFile fp) { bam_header_t *header; char buf[4]; - int32_t i, name_len; + int32_t i = 1, name_len; + // check EOF + i = bgzf_check_EOF(fp); + if (i < 0) { + // If the file is a pipe, checking the EOF marker will *always* fail + // with ESPIPE. Suppress the error message in this case. + if (errno != ESPIPE) perror("[bam_header_read] bgzf_check_EOF"); + } + else if (i == 0) fprintf(stderr, "[bam_header_read] EOF marker is absent.\n"); // read "BAM1" if (bam_read(fp, buf, 4) != 4) return 0; if (strncmp(buf, "BAM\001", 4)) { @@ -267,10 +254,12 @@ char *bam_format1_core(const bam_header_t *header, const bam1_t *b, int of) else if (c->mtid == c->tid) kputs("=\t", &str); else ksprintf(&str, "%s\t", header->target_name[c->mtid]); ksprintf(&str, "%d\t%d\t", c->mpos + 1, c->isize); - for (i = 0; i < c->l_qseq; ++i) kputc(bam_nt16_rev_table[bam1_seqi(s, i)], &str); - kputc('\t', &str); - if (t[0] == 0xff) kputc('*', &str); - else for (i = 0; i < c->l_qseq; ++i) kputc(t[i] + 33, &str); + if (c->l_qseq) { + for (i = 0; i < c->l_qseq; ++i) kputc(bam_nt16_rev_table[bam1_seqi(s, i)], &str); + kputc('\t', &str); + if (t[0] == 0xff) kputc('*', &str); + else for (i = 0; i < c->l_qseq; ++i) kputc(t[i] + 33, &str); + } else ksprintf(&str, "*\t*"); s = bam1_aux(b); while (s < b->data + b->data_len) { uint8_t type, key[2]; @@ -302,3 +291,10 @@ void bam_view1(const bam_header_t *header, const bam1_t *b) printf("%s\n", s); free(s); } + +const char *bam_get_library(const bam_header_t *header, const bam1_t *b) +{ + const uint8_t *rg; + rg = bam_aux_get(b, "RG"); + return (rg == 0)? 0 : sam_tbl_get(header->rg2lib, (const char*)(rg + 1)); +}