X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=README.md;h=7b9a4b83ca83a7ec87926af31cab8bf292a29e8c;hb=1d786dca8b4c9c1e2176a28a669d2d16cd93e395;hp=e59fb357ed34abab97a94ea5aaa1efe8e7bd25e8;hpb=e3d5553e8d1f798a6a36a348ddba475b0f4f1528;p=rsem.git diff --git a/README.md b/README.md index e59fb35..7b9a4b8 100644 --- a/README.md +++ b/README.md @@ -375,13 +375,13 @@ you. Usage: - rsem-find-DE data_matrix_file [--ngvector ngvector_file] number_sample_condition1 FDR_rate output_file + rsem-find-DE data_matrix_file [--ngvector ngvector_file] number_of_samples_in_condition_1 FDR_rate output_file This script calls EBSeq to find differentially expressed genes/transcripts in two conditions. data_matrix_file: m by n matrix containing expected counts, m is the number of transcripts/genes, n is the number of total samples. [--ngvector ngvector_file]: optional field. 'ngvector_file' is calculated by 'rsem-generate-ngvector'. Having this field is recommended for transcript data. -number_sample_condition1: the number of samples in condition 1. A condition's samples must be adjacent. The left group of samples are defined as condition 1. +number_of_samples_in_condition_1: the number of samples in condition 1. A condition's samples must be adjacent. The left group of samples are defined as condition 1. FDR_rate: false discovery rate. output_file: the output file. Three files will be generated: 'output_file', 'output_file.hard_threshold' and 'output_file.all'. The first file reports all DE genes/transcripts using a soft threshold (calculated by crit_func in EBSeq). The second file reports all DE genes/transcripts using a hard threshold (only report if PPEE <= fdr). The third file reports all genes/transcripts. The first file is recommended to be used as DE results because it generally contains more called genes/transcripts. @@ -415,6 +415,8 @@ differential expression analysis. We thank earonesty for contributing patches. +We thank Han Lin for suggesting possible fixes. + ## License RSEM is licensed under the [GNU General Public License