X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=PairedEndQModel.h;h=2c638abed103bcf4f7167b70b60221aa9eafed88;hb=cb94fd597b180aa7cb01ae84c9d1025201b98d8e;hp=2b899c57fa6061e0974e56083da03f580766351d;hpb=3b9adf8b8f6287a38d0aac8487cefb1b2cba6aa0;p=rsem.git

diff --git a/PairedEndQModel.h b/PairedEndQModel.h
index 2b899c5..2c638ab 100644
--- a/PairedEndQModel.h
+++ b/PairedEndQModel.h
@@ -199,8 +199,6 @@ public:
 	}
 
 	int getModelType() const { return model_type; }
-
-	bool simulate(int, PairedEndReadQ&, SingleReadQ&, int&);
  
 private:
 	static const int model_type = 3;
@@ -238,22 +236,27 @@ void PairedEndQModel::estimateFromReads(const char* readFN) {
     for (int i = 0; i < 3; i++)
     	if (N[i] > 0) {
     		genReadFileNames(readFN, i, read_type, s, readFs);
-    		ReadReader<PairedEndReadQ> reader(s, readFs);
+    		ReadReader<PairedEndReadQ> reader(s, readFs, refs->hasPolyA(), seedLen); // allow calculation of calc_lq() function
 
     		int cnt = 0;
     		while (reader.next(read)) {
     			SingleReadQ mate1 = read.getMate1();
     			SingleReadQ mate2 = read.getMate2();
 
-    			mld->update(mate1.getReadLength(), 1.0);
-    			mld->update(mate2.getReadLength(), 1.0);
-
-    			qd->update(mate1.getQScore());
-    			qd->update(mate2.getQScore());
-
-    			if (i == 0) {
-    				nqpro->updateC(mate1.getReadSeq(), mate1.getQScore());
-    				nqpro->updateC(mate2.getReadSeq(), mate2.getQScore());
+    			if (!read.isLowQuality()) {
+    				mld->update(mate1.getReadLength(), 1.0);
+    				mld->update(mate2.getReadLength(), 1.0);
+
+    				qd->update(mate1.getQScore());
+    				qd->update(mate2.getQScore());
+			  
+    				if (i == 0) {
+    					nqpro->updateC(mate1.getReadSeq(), mate1.getQScore());
+    					nqpro->updateC(mate2.getReadSeq(), mate2.getQScore());
+    				}
+    			}
+    			else if (verbose && (mate1.getReadLength() < seedLen || mate2.getReadLength() < seedLen)) {
+    				printf("Warning: Read %s is ignored due to at least one of the mates' length < seed length %d!\n", read.getName().c_str(), seedLen);
     			}
 
     			++cnt;
@@ -300,7 +303,7 @@ void PairedEndQModel::read(const char* inpF) {
 	FILE *fi = fopen(inpF, "r");
 	if (fi == NULL) { fprintf(stderr, "Cannot open %s! It may not exist.\n", inpF); exit(-1); }
 
-	fscanf(fi, "%d", &val);
+	assert(fscanf(fi, "%d", &val) == 1);
 	assert(val == model_type);
 
 	ori->read(fi);
@@ -315,7 +318,7 @@ void PairedEndQModel::read(const char* inpF) {
 		if (M == 0) M = val;
 		if (M == val) {
 			mw = new double[M + 1];
-			for (int i = 0; i <= M; i++) fscanf(fi, "%lf", &mw[i]);
+			for (int i = 0; i <= M; i++) assert(fscanf(fi, "%lf", &mw[i]) == 1);
 		}
 	}
 
@@ -405,9 +408,8 @@ bool PairedEndQModel::simulate(int rid, PairedEndReadQ& read, int& sid) {
 		readseq2 = qpro->simulate(sampler, mateL2, m2pos, m2dir, qual2, ref);
 	}
 
-	std::ostringstream stdout;
-	stdout<<rid<<"_"<<dir<<"_"<<sid<<"_"<<pos<<"_"<<insertL;
-	name = stdout.str();
+	strout<<rid<<"_"<<dir<<"_"<<sid<<"_"<<pos<<"_"<<insertL;
+	name = strout.str();
 
 	read = PairedEndReadQ(SingleReadQ(name + "/1", readseq1, qual1), SingleReadQ(name + "/2", readseq2, qual2));
 
@@ -425,39 +427,39 @@ void PairedEndQModel::finishSimulation() {
 
 
 void PairedEndQModel::calcMW() {
-  assert(seedLen >= OLEN && mld->getMinL() >= seedLen);
-
-  memset(mw, 0, sizeof(double) * (M + 1));
-  mw[0] = 1.0;
-
-  for (int i = 1; i <= M; i++) { 
-    RefSeq& ref = refs->getRef(i);
-    int totLen = ref.getTotLen();
-    int fullLen = ref.getFullLen();
-    int end = std::min(fullLen, totLen - gld->getMinL() + 1);
-    double value = 0.0;
-    int minL, maxL;
-    int effL, pfpos;
-
-    //seedPos is fpos here
-    for (int seedPos = 0; seedPos < end; seedPos++) 
-      if (ref.getMask(seedPos)) {
-	minL = gld->getMinL();
-	maxL = std::min(gld->getMaxL(), totLen - seedPos);
-	pfpos = seedPos;
-	for (int fragLen = minL; fragLen <= maxL; fragLen++) {
-	  effL = std::min(fullLen, totLen - fragLen + 1); 
-	  value += gld->getAdjustedProb(fragLen, totLen) * rspd->getAdjustedProb(pfpos, effL, fullLen); 
-	}
-      }
+	assert(mld->getMinL() >= seedLen);
+
+	memset(mw, 0, sizeof(double) * (M + 1));
+	mw[0] = 1.0;
+
+	for (int i = 1; i <= M; i++) {
+		RefSeq& ref = refs->getRef(i);
+		int totLen = ref.getTotLen();
+		int fullLen = ref.getFullLen();
+		int end = std::min(fullLen, totLen - gld->getMinL() + 1);
+		double value = 0.0;
+		int minL, maxL;
+		int effL, pfpos;
+
+		//seedPos is fpos here
+		for (int seedPos = 0; seedPos < end; seedPos++)
+			if (ref.getMask(seedPos)) {
+				minL = gld->getMinL();
+				maxL = std::min(gld->getMaxL(), totLen - seedPos);
+				pfpos = seedPos;
+				for (int fragLen = minL; fragLen <= maxL; fragLen++) {
+					effL = std::min(fullLen, totLen - fragLen + 1);
+					value += gld->getAdjustedProb(fragLen, totLen) * rspd->getAdjustedProb(pfpos, effL, fullLen);
+				}
+			}
     
-    mw[i] = 1.0 - value;
+		mw[i] = 1.0 - value;
 
-    if (mw[i] < 1e-8) { 
-      //      fprintf(stderr, "Warning: %dth reference sequence is masked for almost all positions!\n", i);
-      mw[i] = 0.0;
-    }
-  }
+		if (mw[i] < 1e-8) {
+			//      fprintf(stderr, "Warning: %dth reference sequence is masked for almost all positions!\n", i);
+			mw[i] = 0.0;
+		}
+	}
 }
 
 #endif /* PAIREDENDQMODEL_H_ */