X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;f=PairedEndModel.h;h=575344c348c6aa1783b66e98a3d760484ec27e22;hb=fb2aa1ca9d00710943155ef3abbbdd87df116e4a;hp=70d14c9de9a97680eb9a683715f887a1d2a1e16f;hpb=fc69cf6af24c0550e55447fc82f01cb6f90c1c42;p=rsem.git diff --git a/PairedEndModel.h b/PairedEndModel.h index 70d14c9..575344c 100644 --- a/PairedEndModel.h +++ b/PairedEndModel.h @@ -10,6 +10,7 @@ #include #include "utils.h" +#include "my_assert.h" #include "Orientation.h" #include "LenDist.h" #include "RSPD.h" @@ -99,7 +100,15 @@ public: int fpos = (dir == 0 ? pos : totLen - pos - insertLen); // the aligned position reported in SAM file, should be a coordinate in forward strand int effL = std::min(fullLen, totLen - insertLen + 1); - assert(fpos >= 0 && fpos + insertLen <= totLen && insertLen <= totLen); + general_assert(fpos >= 0, "The alignment of fragment " + read.getName() + " to transcript " + itos(sid) + " starts at " + itos(fpos) + \ + " from the forward direction, which should be a non-negative number! " + \ + "It is possible that the aligner you use gave different read lengths for a same read in SAM file."); + general_assert(fpos + insertLen <= totLen,"Fragment " + read.getName() + " is hung over the end of transcript " + itos(sid) + "! " \ + + "It is possible that the aligner you use gave different read lengths for a same read in SAM file."); + general_assert(insertLen <= totLen, "Fragment " + read.getName() + " has length " + itos(insertLen) + ", but it is aligned to transcript " \ + + itos(sid) + ", whose length (" + itos(totLen) + ") is shorter than the fragment's length!"); + + if (fpos >= fullLen || ref.getMask(fpos)) return 0.0; // For paired-end model, fpos is the seedPos prob = ori->getProb(dir) * gld->getAdjustedProb(insertLen, totLen) *