X-Git-Url: https://git.donarmstrong.com/?a=blobdiff_plain;ds=sidebyside;f=SingleQModel.h;h=ffc0639909b2f49a5d501c7ecb85be20f2f19f4f;hb=1f6726865dc80d8dd36462c559da46c9e4a8de8a;hp=786d6476383ec41fb06606f74c49099786f693e7;hpb=4c51136f0b5ae7758258642e1183de5bfb4624af;p=rsem.git diff --git a/SingleQModel.h b/SingleQModel.h index 786d647..ffc0639 100644 --- a/SingleQModel.h +++ b/SingleQModel.h @@ -10,6 +10,7 @@ #include #include "utils.h" +#include "my_assert.h" #include "Orientation.h" #include "LenDist.h" #include "RSPD.h" @@ -108,7 +109,14 @@ public: int readLen = read.getReadLength(); int fpos = (dir == 0 ? pos : totLen - pos - readLen); // the aligned position reported in SAM file, should be a coordinate in forward strand - assert(fpos >= 0 && fpos + readLen <= totLen && readLen <= totLen); + general_assert(fpos >= 0, "The alignment of read " + read.getName() + " to transcript " + itos(sid) + " starts at " + itos(fpos) + \ + " from the forward direction, which should be a non-negative number! " + \ + "It is possible that the aligner you use gave different read lengths for a same read in SAM file."); + general_assert(fpos + readLen <= totLen,"Read " + read.getName() + " is hung over the end of transcript " + itos(sid) + "! " \ + + "It is possible that the aligner you use gave different read lengths for a same read in SAM file."); + general_assert(readLen <= totLen, "Read " + read.getName() + " has length " + itos(readLen) + ", but it is aligned to transcript " \ + + itos(sid) + ", whose length (" + itos(totLen) + ") is shorter than the read's length!"); + int seedPos = (dir == 0 ? pos : totLen - pos - seedLen); // the aligned position of the seed in forward strand coordinates if (seedPos >= fullLen || ref.getMask(seedPos)) return 0.0;