tags in the
.B @RG
header lines. Individuals can be pooled in one alignment file; one
-individual can also be separated into multiple files. Similarly, one may
-consider to apply
+individual can also be separated into multiple files. In addition, one
+may consider to apply
.B -C50
to
.BR mpileup .
+SNP calling with mpileup also works for single sample and has the
+advantage of enabling more powerful filtering. The drawback is the lack
+of short indel calling, which may be implemented in future.
+
.IP o 2
Derive the allele frequency spectrum (AFS) on a list of sites from multiple individuals:
.B calmd
command also comes with the
.B -C
-option, the same as the on in
+option, the same as the one in
.B pileup
and
.BR mpileup .
.PP
Heng Li from the Sanger Institute wrote the C version of samtools. Bob
Handsaker from the Broad Institute implemented the BGZF library and Jue
-Ruan from Beijing Genomics Institute wrote the RAZF library. Various
-people in the 1000 Genomes Project contributed to the SAM format
+Ruan from Beijing Genomics Institute wrote the RAZF library. John
+Marshall and Petr Danecek contribute to the source code and various
+people from the 1000 Genomes Project have contributed to the SAM format
specification.
.SH SEE ALSO