}
}
//**********************************************************************************************************************
+string CooccurrenceCommand::getOutputFileNameTag(string type, string inputName=""){
+ try {
+ string outputFileName = "";
+ map<string, vector<string> >::iterator it;
+
+ //is this a type this command creates
+ it = outputTypes.find(type);
+ if (it == outputTypes.end()) { m->mothurOut("[ERROR]: this command doesn't create a " + type + " output file.\n"); }
+ else {
+ if (type == "summary") { outputFileName = "cooccurence.summary"; }
+ else { m->mothurOut("[ERROR]: No definition for type " + type + " output file tag.\n"); m->control_pressed = true; }
+ }
+ return outputFileName;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "CooccurrenceCommand", "getOutputFileNameTag");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
CooccurrenceCommand::CooccurrenceCommand(){
try {
abort = true; calledHelp = true;
set<string> userLabels = labels;
ofstream out;
- string outputFileName = outputDir + m->getRootName(m->getSimpleName(sharedfile)) + "cooccurence.summary";
+ string outputFileName = outputDir + m->getRootName(m->getSimpleName(sharedfile)) + getOutputFileNameTag("summary");
m->openOutputFile(outputFileName, out);
outputNames.push_back(outputFileName); outputTypes["summary"].push_back(outputFileName);
out.setf(ios::fixed, ios::floatfield); out.setf(ios::showpoint);
- out << "metric\tlabel\tScore\tpValue\n";
+ out << "metric\tlabel\tScore\tzScore\tstandardDeviation\n";
//as long as you are not at the end of the file or done wih the lines you want
while((lookup[0] != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
int CooccurrenceCommand::getCooccurrence(vector<SharedRAbundVector*>& thisLookUp, ofstream& out){
try {
int numOTUS = thisLookUp[0]->getNumBins();
+
+ if(numOTUS < 2) {
+ m->mothurOut("Not enough OTUs for co-occurrence analysis, skipping"); m->mothurOutEndLine();
+ return 0;
+ }
+
vector< vector<int> > co_matrix; co_matrix.resize(thisLookUp[0]->getNumBins());
for (int i = 0; i < thisLookUp[0]->getNumBins(); i++) { co_matrix[i].resize((thisLookUp.size()), 0); }
vector<int> columntotal; columntotal.resize(thisLookUp.size(), 0);
}
//populate null matrix from probability matrix, do this a lot.
- for(int h=0;h<runs;h++){
+ for(int k=0;k<runs;k++){
nullmatrix.clear();
//zero-fill the null matrix
nullmatrix.assign(nrows, vector<int>(ncols, 0));
randnum = rand() / double(RAND_MAX);
for(int j=0;j<ncols;j++) {
current = probabilityMatrix[ncols * i + j];
- if((randnum <= current && randnum > previous && nullmatrix[i][j] != 1) || (previous==current)){
+ if(randnum <= current && randnum > previous && nullmatrix[i][j] != 1) {
nullmatrix[i][j] = 1;
count++;
previous = 0.0;
//swap_checkerboards takes the original matrix and swaps checkerboards
else if(matrix == "sim9") {
trial.swap_checkerboards (co_matrix, ncols, nrows);
+ nullmatrix = co_matrix;
}
else {
m->mothurOut("[ERROR]: No null model selected!\n\n"); m->control_pressed = true;
m->mothurOutEndLine(); m->mothurOut("average metric score: " + toString(nullMean)); m->mothurOutEndLine();
+ //calc_p_value is not a statistical p-value, it's just the average that are either > or < the initscore.
+ //All it does is show what is expected in a competitively structured community
+ //zscore is output so p-value can be looked up in a ztable
double pvalue = 0.0;
if (metric == "cscore" || metric == "checker") { pvalue = trial.calc_pvalue_greaterthan (stats, initscore); }
else{ pvalue = trial.calc_pvalue_lessthan (stats, initscore); }
+
+ double sd = trial.getSD(runs, stats, nullMean);
+
+ double zscore = trial.get_zscore(sd, nullMean, initscore);
- m->mothurOut("pvalue: " + toString(pvalue)); m->mothurOutEndLine();
- out << metric << '\t' << thisLookUp[0]->getLabel() << '\t' << nullMean << '\t' << pvalue << endl;
+ m->mothurOut("zscore: " + toString(zscore)); m->mothurOutEndLine();
+ m->mothurOut("standard deviation: " + toString(sd)); m->mothurOutEndLine();
+ out << metric << '\t' << thisLookUp[0]->getLabel() << '\t' << nullMean << '\t' << zscore << '\t' << sd << endl;
return 0;
}