assert_raise(SeqError) { @entry.subseq(-1, 1) }
end
- def test_Seq_subseq_with_length_lt_1_raises
- @entry.seq = "ATCG"
- assert_raise(SeqError) { @entry.subseq(0, 0) }
- end
-
def test_Seq_subseq_with_start_plus_length_gt_seq_raises
@entry.seq = "ATCG"
assert_raise(SeqError) { @entry.subseq(0, 5) }
assert_raise(SeqError) { @entry.subseq!(-1, 1) }
end
- def test_Seq_subseq_bang_with_length_lt_1_raises
- @entry.seq = "ATCG"
- assert_raise(SeqError) { @entry.subseq!(0, 0) }
- end
-
def test_Seq_subseq_bang_with_start_plus_length_gt_seq_raises
@entry.seq = "ATCG"
assert_raise(SeqError) { @entry.subseq!(0, 5) }
assert_equal("ATCG", @entry.subseq_rand(4).seq)
end
- def test_Seq_quality_trim_right_with_missing_seq_raises
- @entry.qual = "hhhh"
- assert_raise(SeqError) { @entry.quality_trim_right(20) }
- end
-
- def test_Seq_quality_trim_right_with_missing_qual_raises
- @entry.seq = "ATCG"
- assert_raise(SeqError) { @entry.quality_trim_right(20) }
- end
-
- def test_Seq_quality_trim_right_with_bad_min_raises
- @entry.seq = "ATCG"
- @entry.qual = "hhhh"
-
- [-1, 41].each do |min|
- assert_raise(SeqError) { @entry.quality_trim_right(min) }
- end
- end
-
- def test_Seq_quality_trim_right_with_ok_min_dont_raise
- @entry.seq = "ATCG"
- @entry.qual = "hhhh"
-
- [0, 40].each do |min|
- assert_nothing_raised { @entry.quality_trim_right(min) }
- end
- end
-
- def test_Seq_quality_trim_right_returns_correctly
- @entry.seq = "AAAAATCG"
- @entry.qual = "hhhhhgfe"
- @entry.quality_trim_right(38)
- assert_equal("AAAAAT", @entry.seq)
- assert_equal("hhhhhg", @entry.qual)
- end
-
- def test_Seq_quality_trim_left_with_missing_seq_raises
- @entry.qual = "hhhh"
- assert_raise(SeqError) { @entry.quality_trim_left(20) }
- end
-
- def test_Seq_quality_trim_left_with_missing_qual_raises
- @entry.seq = "ATCG"
- assert_raise(SeqError) { @entry.quality_trim_left(20) }
- end
-
- def test_Seq_quality_trim_left_with_bad_min_raises
- @entry.seq = "ATCG"
- @entry.qual = "hhhh"
-
- [-1, 41].each do |min|
- assert_raise(SeqError) { @entry.quality_trim_left(min) }
- end
- end
-
- def test_Seq_quality_trim_left_with_ok_min_dont_raise
- @entry.seq = "ATCG"
- @entry.qual = "hhhh"
-
- [0, 40].each do |min|
- assert_nothing_raised { @entry.quality_trim_left(min) }
- end
- end
-
- def test_Seq_quality_trim_left_returns_correctly
- @entry.seq = "GCTAAAAA"
- @entry.qual = "efghhhhh"
- @entry.quality_trim_left(38)
- assert_equal("TAAAAA", @entry.seq)
- assert_equal("ghhhhh", @entry.qual)
- end
-
- def test_Seq_quality_trim_returns_correctly
- @entry.seq = "GCTAAAAAGTG"
- @entry.qual = "efghhhhhgfe"
- @entry.quality_trim(38)
- assert_equal("TAAAAAG", @entry.seq)
- assert_equal("ghhhhhg", @entry.qual)
- end
-
def test_Seq_indels_remove_without_qual_returns_correctly
@entry.seq = "A-T.CG~CG"
@entry.qual = nil
end
def test_Seq_mask_seq_hard_bang_returns_correctly
- @entry.seq = "ATCG"
- @entry.qual = "RSTU"
+ @entry.seq = "-ATCG"
+ @entry.qual = "RRSTU"
- assert_equal("NNCG", @entry.mask_seq_hard!(20).seq)
+ assert_equal("-NNCG", @entry.mask_seq_hard!(20).seq)
end
def test_Seq_mask_seq_soft_bang_with_nil_seq_raises
assert_raise(SeqError) { @entry.mask_seq_soft!(20) }
end
- def test_Seq_mask_seq_soft_bang_returns_correctly
- @entry.seq = "ATCG"
- @entry.qual = "RSTU"
-
- assert_equal("atCG", @entry.mask_seq_soft!(20).seq)
- end
-
def test_Seq_mask_seq_soft_bang_with_bad_cutoff_raises
assert_raise(SeqError) { @entry.mask_seq_soft!(-1) }
assert_raise(SeqError) { @entry.mask_seq_soft!(41) }
assert_nothing_raised { @entry.mask_seq_soft!(0) }
assert_nothing_raised { @entry.mask_seq_soft!(40) }
end
-end
+ def test_Seq_mask_seq_soft_bang_returns_correctly
+ @entry.seq = "-ATCG"
+ @entry.qual = "RRSTU"
+
+ assert_equal("-atCG", @entry.mask_seq_soft!(20).seq)
+ end
+
+ # qual score detection
+
+ def test_Seq_qual_base33_returns_correctly
+ # self.qual.match(/[!-:]/)
+ @entry.qual = '!"#$%&\'()*+,-./0123456789:'
+ assert_equal(true, @entry.qual_base33? )
+ @entry.qual = 32.chr
+ assert_equal(false, @entry.qual_base33? )
+ @entry.qual = 59.chr
+ assert_equal(false, @entry.qual_base33? )
+ end
+
+ def test_Seq_qual_base64_returns_correctly
+ # self.qual.match(/[K-h]/)
+ @entry.qual = 'KLMNOPQRSTUVWXYZ[\]^_`abcdefgh'
+ assert_equal(true, @entry.qual_base64? )
+ @entry.qual = 74.chr
+ assert_equal(false, @entry.qual_base64? )
+ @entry.qual = 105.chr
+ assert_equal(false, @entry.qual_base64? )
+ end
+
+ def test_Seq_qual_valid_with_nil_qual_raises
+ assert_raise(SeqError) { @entry.qual_valid?("illumina1.8") }
+ end
+
+ def test_Seq_qual_valid_with_bad_encoding_raises
+ @entry.qual = "abc"
+ assert_raise(SeqError) { @entry.qual_valid?("foobar") }
+ end
+
+ def test_Seq_qual_valid_returns_correctly
+ tests = [["sanger", 0, 40, 33],
+ ["454", 0, 40, 64],
+ ["solexa", -5, 40, 64],
+ ["illumina13", 0, 40, 64],
+ ["illumina15", 0, 40, 64],
+ ["illumina18", 0, 41, 33]]
+
+ tests.each do |test|
+ @entry.qual = (test[1] + test[-1]).chr + (test[2] + test[-1]).chr
+ assert_equal(true, @entry.qual_valid?(test[0]))
+ @entry.qual = (test[1] + test[-1] - 1).chr
+ assert_equal(false, @entry.qual_valid?(test[0]))
+ @entry.qual = (test[2] + test[-1] + 1).chr
+ assert_equal(false, @entry.qual_valid?(test[0]))
+ end
+ end
+
+ # convert sanger to ...
+
+ def test_Seq_convert_scores_bang_from_sanger_to_sanger_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('sanger', 'sanger').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_sanger_to_solexa_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('abcdefgh', @entry.convert_scores!('sanger', 'solexa').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_sanger_to_illumina13_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('abcdefgh', @entry.convert_scores!('sanger', 'illumina13').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_sanger_to_illumina15_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('abcdefgh', @entry.convert_scores!('sanger', 'illumina15').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_sanger_to_illumina18_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('sanger', 'illumina18').qual)
+ end
+
+ # convert solexa to ...
+
+ def test_Seq_convert_scores_bang_from_solexa_to_sanger_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal(%q[#$%&'()*], @entry.convert_scores!('solexa', 'sanger').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_solexa_to_solexa_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('solexa', 'solexa').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_solexa_to_illumina13_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('solexa', 'illumina13').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_solexa_to_illumina15_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('solexa', 'illumina15').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_solexa_to_illumina18_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal(%q[#$%&'()*], @entry.convert_scores!('solexa', 'illumina18').qual)
+ end
+
+ # convert illumina13 to ...
+
+ def test_Seq_convert_scores_bang_from_illumina13_to_sanger_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal(%q[#$%&'()*], @entry.convert_scores!('illumina13', 'sanger').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina13_to_solexa_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('illumina13', 'solexa').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina13_to_illumina13_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('illumina13', 'illumina13').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina13_to_illumina15_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('illumina13', 'illumina15').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina13_to_illumina18_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal(%q[#$%&'()*], @entry.convert_scores!('illumina13', 'illumina18').qual)
+ end
+
+ # convert illumina15 to ...
+
+ def test_Seq_convert_scores_bang_from_illumina15_to_sanger_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal(%q[#$%&'()*], @entry.convert_scores!('illumina15', 'sanger').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina15_to_solexa_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('illumina15', 'solexa').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina15_to_illumina13_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('illumina15', 'illumina13').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina15_to_illumina15_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('illumina15', 'illumina15').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina15_to_illumina18_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal(%q[#$%&'()*], @entry.convert_scores!('illumina15', 'illumina18').qual)
+ end
+
+ # convert illumina18 to ...
+
+ def test_Seq_convert_scores_bang_from_illumina18_to_sanger_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('illumina18', 'sanger').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina18_to_solexa_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('abcdefgh', @entry.convert_scores!('illumina18', 'solexa').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina18_to_illumina13_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('abcdefgh', @entry.convert_scores!('illumina18', 'illumina13').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina18_to_illumina15_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('abcdefgh', @entry.convert_scores!('illumina18', 'illumina15').qual)
+ end
+
+ def test_Seq_convert_scores_bang_from_illumina18_to_illumina18_returns_OK
+ @entry.qual = 'BCDEFGHI'
+ assert_equal('BCDEFGHI', @entry.convert_scores!('illumina18', 'illumina18').qual)
+ end
+
+ def test_Seq_scores_mean_without_qual_raises
+ @entry.qual = nil
+ assert_raise(SeqError) { @entry.scores_mean }
+ end
+
+ def test_Seq_scores_mean_returns_correctly
+ @entry.qual = '@@hh'
+ assert_equal(20.0, @entry.scores_mean)
+ end
+end
__END__
projects / biopieces.git / blobdiff